WO2003008639A3 - Procede de detection d'un acide nucleique - Google Patents

Procede de detection d'un acide nucleique Download PDF

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Publication number
WO2003008639A3
WO2003008639A3 PCT/EP2002/007801 EP0207801W WO03008639A3 WO 2003008639 A3 WO2003008639 A3 WO 2003008639A3 EP 0207801 W EP0207801 W EP 0207801W WO 03008639 A3 WO03008639 A3 WO 03008639A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detecting
fluorophore
acceptor
probe
Prior art date
Application number
PCT/EP2002/007801
Other languages
German (de)
English (en)
Other versions
WO2003008639A2 (fr
Inventor
Thomas Schmitt-John
Regina Plessow
Ralf Palmisano
Andreas Brockhinke
Juergen Weidner
Original Assignee
Praenadia Gmbh
Thomas Schmitt-John
Regina Plessow
Ralf Palmisano
Andreas Brockhinke
Juergen Weidner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Praenadia Gmbh, Thomas Schmitt-John, Regina Plessow, Ralf Palmisano, Andreas Brockhinke, Juergen Weidner filed Critical Praenadia Gmbh
Publication of WO2003008639A2 publication Critical patent/WO2003008639A2/fr
Publication of WO2003008639A3 publication Critical patent/WO2003008639A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé de détection d'un acide nucléique. Selon ce procédé, l'acide nucléique est hybridé avec au moins une première sonde comprenant un fluorogène donneur et une deuxième sonde comprenant un fluorogène accepteur, le spectre d'émission du fluorogène donneur chevauchant au moins le spectre d'excitation du fluorogène accepteur. Le fluorogène donneur est ensuite excité sélectivement et l'émission du fluorogène accepteur est détectée.
PCT/EP2002/007801 2001-07-12 2002-07-12 Procede de detection d'un acide nucleique WO2003008639A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10133308.0 2001-07-12
DE10133308A DE10133308A1 (de) 2001-07-12 2001-07-12 Verfahren zum Nachweis einer Nukleinsäure

Publications (2)

Publication Number Publication Date
WO2003008639A2 WO2003008639A2 (fr) 2003-01-30
WO2003008639A3 true WO2003008639A3 (fr) 2003-09-18

Family

ID=7691175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007801 WO2003008639A2 (fr) 2001-07-12 2002-07-12 Procede de detection d'un acide nucleique

Country Status (2)

Country Link
DE (1) DE10133308A1 (fr)
WO (1) WO2003008639A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101809171A (zh) * 2007-08-03 2010-08-18 生物概念股份有限公司 原位杂交以检测rna和dna标记

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003431A1 (fr) * 1993-07-19 1995-02-02 Aprogenex, Inc. Enrichissement et identification de cellules f×tales dans du sang maternel destinees a une hybridation in situ
EP0745690A2 (fr) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Trousse de réactives, procédé et sondes oligonucléotides marquées de conformation dual
US5728527A (en) * 1993-07-20 1998-03-17 University Of Massachusetts Medical Center Detection of hybridized oligonocleotide probes in living cells
US5804386A (en) * 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
EP1067134A2 (fr) * 1991-11-07 2001-01-10 Nanotronics, Inc. Hybridation de polynucleotides conjugués à des chromophores et àdes fluorophores afin de générer des systèmes de transfert d'énergie de donneur à donneur
US6228592B1 (en) * 1999-05-12 2001-05-08 Laboratory Of Molecular Biophotonics Nucleic acid detection in cytoplasm
EP1172445A1 (fr) * 2000-07-14 2002-01-16 Praenadia GmbH Procede d'analyse genetique directe de cellules cible utilisant sondes fluorescentes

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1067134A2 (fr) * 1991-11-07 2001-01-10 Nanotronics, Inc. Hybridation de polynucleotides conjugués à des chromophores et àdes fluorophores afin de générer des systèmes de transfert d'énergie de donneur à donneur
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
WO1995003431A1 (fr) * 1993-07-19 1995-02-02 Aprogenex, Inc. Enrichissement et identification de cellules f×tales dans du sang maternel destinees a une hybridation in situ
US5728527A (en) * 1993-07-20 1998-03-17 University Of Massachusetts Medical Center Detection of hybridized oligonocleotide probes in living cells
EP0745690A2 (fr) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Trousse de réactives, procédé et sondes oligonucléotides marquées de conformation dual
US5804386A (en) * 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US6228592B1 (en) * 1999-05-12 2001-05-08 Laboratory Of Molecular Biophotonics Nucleic acid detection in cytoplasm
EP1172445A1 (fr) * 2000-07-14 2002-01-16 Praenadia GmbH Procede d'analyse genetique directe de cellules cible utilisant sondes fluorescentes

Also Published As

Publication number Publication date
WO2003008639A2 (fr) 2003-01-30
DE10133308A1 (de) 2003-01-23

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