WO2003004636A3 - Expression vectors encoding bacteriophage signal peptides - Google Patents

Expression vectors encoding bacteriophage signal peptides Download PDF

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Publication number
WO2003004636A3
WO2003004636A3 PCT/GB2002/003129 GB0203129W WO03004636A3 WO 2003004636 A3 WO2003004636 A3 WO 2003004636A3 GB 0203129 W GB0203129 W GB 0203129W WO 03004636 A3 WO03004636 A3 WO 03004636A3
Authority
WO
WIPO (PCT)
Prior art keywords
signal peptides
expression vectors
vectors encoding
encoding bacteriophage
bacteriophage signal
Prior art date
Application number
PCT/GB2002/003129
Other languages
French (fr)
Other versions
WO2003004636A2 (en
Inventor
David Paul Humphreys
Original Assignee
Celltech R&D Ltd
David Paul Humphreys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech R&D Ltd, David Paul Humphreys filed Critical Celltech R&D Ltd
Priority to EP02743424A priority Critical patent/EP1402036B1/en
Priority to AU2002345213A priority patent/AU2002345213A1/en
Priority to DE60224930T priority patent/DE60224930T2/en
Priority to JP2003510795A priority patent/JP2004533264A/en
Priority to CA002452359A priority patent/CA2452359A1/en
Priority to US10/483,008 priority patent/US7244587B2/en
Publication of WO2003004636A2 publication Critical patent/WO2003004636A2/en
Publication of WO2003004636A3 publication Critical patent/WO2003004636A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

Expression vectors encoding bacteriophage signal peptides are described. The vectors may be used for the heterologous expression and secretion of polypeptides such as antibodies in bacterial host cells.
PCT/GB2002/003129 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides WO2003004636A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP02743424A EP1402036B1 (en) 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides
AU2002345213A AU2002345213A1 (en) 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides
DE60224930T DE60224930T2 (en) 2001-07-05 2002-07-05 EXPRESSION VECTORS ENCODING THE SIGNAL PEPTIDES FROM BACTERIOPHAGES
JP2003510795A JP2004533264A (en) 2001-07-05 2002-07-05 Expression vector encoding bacteriophage signal peptide
CA002452359A CA2452359A1 (en) 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides
US10/483,008 US7244587B2 (en) 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0116460.7 2001-07-05
GBGB0116460.7A GB0116460D0 (en) 2001-07-05 2001-07-05 Biological products

Publications (2)

Publication Number Publication Date
WO2003004636A2 WO2003004636A2 (en) 2003-01-16
WO2003004636A3 true WO2003004636A3 (en) 2003-06-26

Family

ID=9917985

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/003129 WO2003004636A2 (en) 2001-07-05 2002-07-05 Expression vectors encoding bacteriophage signal peptides

Country Status (10)

Country Link
US (1) US7244587B2 (en)
EP (1) EP1402036B1 (en)
JP (1) JP2004533264A (en)
AT (1) ATE385516T1 (en)
AU (1) AU2002345213A1 (en)
CA (1) CA2452359A1 (en)
DE (1) DE60224930T2 (en)
ES (1) ES2299582T3 (en)
GB (1) GB0116460D0 (en)
WO (1) WO2003004636A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7527947B2 (en) 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
WO2005121333A1 (en) * 2004-06-14 2005-12-22 Novozymes A/S Signal peptide for producing a polypeptide
CN103451164A (en) * 2006-07-14 2013-12-18 诺维信股份有限公司 Methods for producing secreted polypeptides having biological activity
KR101698040B1 (en) * 2013-06-27 2017-01-24 주식회사 중겸 Single domain antibody for M13 bacteriophage and immunosensor fusion protein comprising the same
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
WO2018174817A1 (en) * 2017-03-22 2018-09-27 Agency For Science, Technology And Research Protein expression construct and methods thereof
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997001580A1 (en) * 1995-06-27 1997-01-16 The Scripps Research Institute Multispecific and multivalent antigen-binding polypeptide molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997001580A1 (en) * 1995-06-27 1997-01-16 The Scripps Research Institute Multispecific and multivalent antigen-binding polypeptide molecules

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HUMPHREYS DP AND GLOVER DJ: "Therapeutic antibody production technologies: molecules, applications, expression and purification.", CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT. ENGLAND MAR 2001, vol. 4, no. 2, March 2001 (2001-03-01), pages 172 - 185, XP008016086, ISSN: 1367-6733 *
HUMPHREYS DP ET AL: "A plasmid system for optimization of Fab' production in Escherichia coli: Importance of balance of heavy chain and light chain synthesis.", PROTEIN EXPRESSION AND PURIFICATION, vol. 26, no. 2, 20 November 2002 (2002-11-20), pages 309 - 320, XP002238216, ISSN: 1046-5928 *
HUMPHREYS DP ET AL: "High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence.", PROTEIN EXPRESSION AND PURIFICATION. UNITED STATES NOV 2000, vol. 20, no. 2, November 2000 (2000-11-01), pages 252 - 264, XP002238215, ISSN: 1046-5928 *
MARKLAND W ET AL: "DESIGN CONSTRUCTION AND FUNCTION OF A MULTICOPY DISPLAY VECTOR USING FUSIONS TO THE MAJOR COAT PROTEIN OF BACTERIOPHAGE M13", GENE (AMSTERDAM), vol. 109, no. 1, 1991, pages 13 - 20, XP000575848, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
EP1402036A2 (en) 2004-03-31
ATE385516T1 (en) 2008-02-15
ES2299582T3 (en) 2008-06-01
US20040259210A1 (en) 2004-12-23
EP1402036B1 (en) 2008-02-06
US7244587B2 (en) 2007-07-17
CA2452359A1 (en) 2003-01-16
DE60224930T2 (en) 2009-02-05
GB0116460D0 (en) 2001-08-29
DE60224930D1 (en) 2008-03-20
WO2003004636A2 (en) 2003-01-16
JP2004533264A (en) 2004-11-04
AU2002345213A1 (en) 2003-01-21

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