AU2002311620A1 - Methods of making and using expression constructs for the expression of recombinant proteins - Google Patents

Methods of making and using expression constructs for the expression of recombinant proteins

Info

Publication number
AU2002311620A1
AU2002311620A1 AU2002311620A AU2002311620A AU2002311620A1 AU 2002311620 A1 AU2002311620 A1 AU 2002311620A1 AU 2002311620 A AU2002311620 A AU 2002311620A AU 2002311620 A AU2002311620 A AU 2002311620A AU 2002311620 A1 AU2002311620 A1 AU 2002311620A1
Authority
AU
Australia
Prior art keywords
expression
making
methods
recombinant proteins
constructs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002311620A
Inventor
Zeev Pancer
Yoav Peleg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Insight Biopharmaceuticals Ltd
Original Assignee
Insight Biopharmaceuticals Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Insight Biopharmaceuticals Ltd filed Critical Insight Biopharmaceuticals Ltd
Publication of AU2002311620A1 publication Critical patent/AU2002311620A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AU2002311620A 2001-07-02 2002-07-02 Methods of making and using expression constructs for the expression of recombinant proteins Abandoned AU2002311620A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30180501P 2001-07-02 2001-07-02
US60/301,805 2001-07-02
PCT/IL2002/000540 WO2003004599A2 (en) 2001-07-02 2002-07-02 Methods of making and using expression constructs for the expression of recombinant proteins

Publications (1)

Publication Number Publication Date
AU2002311620A1 true AU2002311620A1 (en) 2003-01-21

Family

ID=23164962

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002311620A Abandoned AU2002311620A1 (en) 2001-07-02 2002-07-02 Methods of making and using expression constructs for the expression of recombinant proteins

Country Status (2)

Country Link
AU (1) AU2002311620A1 (en)
WO (1) WO2003004599A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2281822T3 (en) * 2003-08-13 2007-10-01 Sandoz Ag EXPRESSION VECTORS, TRANSFORMED GUEST CELLS AND FERMENTATION PROCEDURE FOR THE PRODUCTION OF RECOMBINATING POLYPEPTIDES.
ES2391457T3 (en) * 2003-08-13 2012-11-26 Sandoz Ag Procedure for purification of recombinant polypeptides
GB0319601D0 (en) * 2003-08-20 2003-09-24 Sandoz Ag Production process
CN102766645B (en) * 2012-06-08 2013-06-05 武汉凯肽来生物科技有限公司 Oral recombinant protein TAT-GH for promoting ricefield eel growth, and preparation method and application thereof
WO2014084432A1 (en) * 2012-11-30 2014-06-05 경상대학교 산학협력단 Helicobacter pylori expression vector
CN111705032B (en) * 2020-07-09 2022-04-05 科赫生物科技（北京）有限公司 Prokaryotic expression cell and preparation method and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030018001A1 (en) * 1996-11-21 2003-01-23 Heaney Anthony P. Methods of using pituitary tumor transforming gene (PTTG) carboxy-terminal peptides to inhibit neoplastic cellular proliferation and/or transformation of breast and ovarian cells
EP1037911A4 (en) * 1997-12-10 2003-07-23 Univ Washington Anti-pathogen system and methods of use thereof
AU7577600A (en) * 1999-09-13 2001-04-17 Cornell Research Foundation Inc. Delivering to eucaryotic cells bacterial proteins that are secreted via type iiisecretion systems

Also Published As

Publication number Publication date
WO2003004599A2 (en) 2003-01-16
WO2003004599A3 (en) 2004-03-18

Similar Documents

Publication Publication Date Title
AU2002362197A1 (en) Method of stabilizing protein
WO2003003984A9 (en) Novel proteins and nucleic acids encoding same
AU2002340167A1 (en) Anti-hla-dr antibodies and the methods of using thereof
AU2002236300A1 (en) Novel protein, gene encoding the same and method of using the same
AU2002232819A1 (en) Methods for the production of multimeric proteins, and related compositions
AU2002217505A1 (en) Process for producing recombinant protein and fused protein
EP1441030A4 (en) Method of purifying recombinant fused protein and method of producing protein using the same
AU2002251999A1 (en) Methods and compositions for the construction and use of fusion libraries using computational protein design methods
AU2002354560A1 (en) Targeted fusion proteins and methods for the characterization of cellular membrane domains
AU2002311620A1 (en) Methods of making and using expression constructs for the expression of recombinant proteins
AU2002258525A1 (en) Telomerase expression repressor proteins and methods of using the same
AU2003276844A1 (en) Formulations of modified antibodies and methods of making the same
AU2002345743A1 (en) Telomerase expression repressor proteins and methods of using the same
EP1572995A3 (en) Novel proteins and nucleic acids encoding same
AU2002341828A1 (en) Enhanced proteins and methods for their use
AU2002347761A1 (en) Novel polypeptide analogs and fusions and their methods of use
AU2002321440A1 (en) Expression of modified antibodies in avian cells
AU2002354363A1 (en) Method of activating protein
AU2001284651A1 (en) Secreted proteins and methods of using same
AU2002254595A1 (en) Method for enhancing the translation and expression of recombinant proteins
AU2002316348A1 (en) Chimeric capsid proteins and uses thereof
AU2003226907A1 (en) Method for the preparation of recombinant mammalian heparin-binding protein (hbp)
AU2002344252A1 (en) Mammalian diabetes-mediating proteins
AU2002213954A1 (en) Method for the selective modification of peptides and proteins
AU2002240921A1 (en) Medane genes and proteins

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase