WO2003002590A3 - A protein folding reactor - Google Patents

A protein folding reactor Download PDF

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Publication number
WO2003002590A3
WO2003002590A3 PCT/GB2002/003017 GB0203017W WO03002590A3 WO 2003002590 A3 WO2003002590 A3 WO 2003002590A3 GB 0203017 W GB0203017 W GB 0203017W WO 03002590 A3 WO03002590 A3 WO 03002590A3
Authority
WO
WIPO (PCT)
Prior art keywords
mixing
protein
feed point
protein folding
mixing chamber
Prior art date
Application number
PCT/GB2002/003017
Other languages
French (fr)
Other versions
WO2003002590A2 (en
Inventor
Anton Middelberg
Chew Tin Lee
Malcolm MACKLEY
Mark Buswell
Original Assignee
Anton Middelberg
Chew Tin Lee
Malcolm MACKLEY
Mark Buswell
Univ Cambridge Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anton Middelberg, Chew Tin Lee, Malcolm MACKLEY, Mark Buswell, Univ Cambridge Tech filed Critical Anton Middelberg
Priority to AU2002314356A priority Critical patent/AU2002314356A1/en
Publication of WO2003002590A2 publication Critical patent/WO2003002590A2/en
Publication of WO2003002590A3 publication Critical patent/WO2003002590A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/18—Stationary reactors having moving elements inside
    • B01J19/1868—Stationary reactors having moving elements inside resulting in a loop-type movement
    • B01J19/1881—Stationary reactors having moving elements inside resulting in a loop-type movement externally, i.e. the mixture leaving the vessel and subsequently re-entering it
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/82—Combinations of dissimilar mixers
    • B01F33/821—Combinations of dissimilar mixers with consecutive receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/712—Feed mechanisms for feeding fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/18—Stationary reactors having moving elements inside
    • B01J19/1893—Membrane reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/28—Moving reactors, e.g. rotary drums
    • B01J19/285—Shaking or vibrating reactors; reactions under the influence of low-frequency vibrations or pulsations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00002—Chemical plants
    • B01J2219/00027—Process aspects
    • B01J2219/00033—Continuous processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00761—Details of the reactor
    • B01J2219/00763—Baffles
    • B01J2219/00765—Baffles attached to the reactor wall
    • B01J2219/00777—Baffles attached to the reactor wall horizontal

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A protein folding reactor comprising: a first mixing chamber for holding a folding buffer; one or more of feeders for feeding denatured protein into the first mixing chamber, each feeder feeding at a respective feed point; and a first mixing mechanism capable of effecting high intensity mixing at each feed point in the first mixing chamber. The intensity of mixing adjacent each feed point is such that there is an increased yield of active protein when denatured protein is fed into the folding buffer.
PCT/GB2002/003017 2001-06-29 2002-06-28 A protein folding reactor WO2003002590A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002314356A AU2002314356A1 (en) 2001-06-29 2002-06-28 A protein folding reactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0116038.1 2001-06-29
GBGB0116038.1A GB0116038D0 (en) 2001-06-29 2001-06-29 A protein folding reactor

Publications (2)

Publication Number Publication Date
WO2003002590A2 WO2003002590A2 (en) 2003-01-09
WO2003002590A3 true WO2003002590A3 (en) 2003-05-01

Family

ID=9917692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/003017 WO2003002590A2 (en) 2001-06-29 2002-06-28 A protein folding reactor

Country Status (3)

Country Link
AU (1) AU2002314356A1 (en)
GB (1) GB0116038D0 (en)
WO (1) WO2003002590A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005009322A1 (en) * 2005-03-01 2006-09-14 Degussa Ag Mixing reactor
US7810674B2 (en) 2005-07-26 2010-10-12 Millipore Corporation Liquid dispensing system with enhanced mixing
US7950547B2 (en) 2006-01-12 2011-05-31 Millipore Corporation Reservoir for liquid dispensing system with enhanced mixing
IN2013MU02416A (en) 2013-07-19 2015-06-19 Biogenomics Ltd
FR3068361B1 (en) * 2017-06-30 2021-10-15 Univ Paris Diderot Paris 7 FLUIDIC SYSTEM FOR THE PRODUCTION OF EXTRACELLULAR VESICLES AND ASSOCIATED PROCESS
CN114307705A (en) * 2021-12-09 2022-04-12 大连理工大学 Bionic flexible reactor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2101979A5 (en) * 1970-08-05 1972-03-31 Otto & Co Gmbh Dr C Pulsation column - with external double acting pulsation piston connected above and below exchange section
GB2283209A (en) * 1993-08-12 1995-05-03 Robert Dennis Dickinson Security strap
FR2741345A1 (en) * 1995-11-20 1997-05-23 Agrichimie Sa Selective condensation of aromatic and carbonyl compounds in multi-contact counterflow reactor
JP2000229995A (en) * 1999-02-12 2000-08-22 Ngk Insulators Ltd Method and apparatus for regenerating protein

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2101979A5 (en) * 1970-08-05 1972-03-31 Otto & Co Gmbh Dr C Pulsation column - with external double acting pulsation piston connected above and below exchange section
GB2283209A (en) * 1993-08-12 1995-05-03 Robert Dennis Dickinson Security strap
FR2741345A1 (en) * 1995-11-20 1997-05-23 Agrichimie Sa Selective condensation of aromatic and carbonyl compounds in multi-contact counterflow reactor
JP2000229995A (en) * 1999-02-12 2000-08-22 Ngk Insulators Ltd Method and apparatus for regenerating protein

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
C. T. LEE ET AL.: "Protein refolding in an oscillatory flow reactor", BIOTECHNOLOGY LETTERS, vol. 23, 2001, KEW, SURREY, GB, pages 1899 - 1901, XP008012600 *
CHEW T. LEE ET AL.: "The influence of mixing on lysozyme renaturation during refolding in an oscillatory flow and a stirred-tank reactor", CHEMICAL ENGINEERING SCIENCE, vol. 57, no. 10, May 2002 (2002-05-01), , OXFORD, GB, pages 1679 - 1684, XP004354498, ISSN: 0009-2509 *
DATABASE WPI Section Ch Week 200058, Derwent World Patents Index; Class B04, AN 2000-605238, XP002228259 *
JE-NIE PHUE ET AL.: "Improved Refolding of Recombinant Human Proinsulin from Escherichia coli in a Two-Stage Reactor", JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, vol. 10, no. 1, 2000, SOUTH KOREA, pages 75 - 80, XP008012601 *
NICHOLAS KOTLARSKI ET AL.: "Design Analysis for Refolding Monomeric Protein", AICHE JOURNAL, vol. 43, no. 8, August 1997 (1997-08-01), New York, New York, USA, pages 2123 - 2132, XP008012599 *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 11 3 January 2001 (2001-01-03) *

Also Published As

Publication number Publication date
GB0116038D0 (en) 2001-08-22
AU2002314356A1 (en) 2003-03-03
WO2003002590A2 (en) 2003-01-09

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