IN2013MU02416A - - Google Patents

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Publication number
IN2013MU02416A
IN2013MU02416A IN2416MU2013A IN2013MU02416A IN 2013MU02416 A IN2013MU02416 A IN 2013MU02416A IN 2416MU2013 A IN2416MU2013 A IN 2416MU2013A IN 2013MU02416 A IN2013MU02416 A IN 2013MU02416A
Authority
IN
India
Prior art keywords
refolding
tank
solubilisation
vessels
feed
Prior art date
Application number
Inventor
Sanjay Sonar
Archana Krishnan
Nikhil Ghade
Faiza Shaikh
Original Assignee
Biogenomics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogenomics Ltd filed Critical Biogenomics Ltd
Priority to US14/905,438 priority Critical patent/US20160159853A1/en
Priority to EP14789629.4A priority patent/EP3022218A1/en
Priority to SG11201600380VA priority patent/SG11201600380VA/en
Priority to PCT/IN2014/000476 priority patent/WO2015008302A1/en
Priority to IN2416MU2013 priority patent/IN2013MU02416A/en
Publication of IN2013MU02416A publication Critical patent/IN2013MU02416A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/24—Stationary reactors without moving elements inside
    • B01J19/245—Stationary reactors without moving elements inside placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/24—Stationary reactors without moving elements inside

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A machine or apparatus for refolding a protein of interest produced recombinantly in a host cell in form of inclusion bodies is provided. The apparatus includes an inclusion bodies (IB) solubilisation tank to solubilise the inclusion bodies; a plurality of refolding vessels or reactors arranged in series to receive a refolding feed; a first tank connected to the IB solubilisation tank to hold diafiltration buffer or agent, at least one diafiltration cartridge, having at least one permeate end and one retentate end, connected to the IB solubilisation tank, through a first retentate end, to feed the refolding feed to the IB solubilisation tank; a second tank, connected to the first permeate end of the diafiltration cartridge to receive and recycle the refolding buffer and each of the refolding vessels, for supplying refolding buffer or agent to each of the refolding vessels.
IN2416MU2013 2013-07-19 2014-07-18 IN2013MU02416A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US14/905,438 US20160159853A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
EP14789629.4A EP3022218A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
SG11201600380VA SG11201600380VA (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
PCT/IN2014/000476 WO2015008302A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18

Publications (1)

Publication Number Publication Date
IN2013MU02416A true IN2013MU02416A (en) 2015-06-19

Family

ID=51795686

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18

Country Status (5)

Country Link
US (1) US20160159853A1 (en)
EP (1) EP3022218A1 (en)
IN (1) IN2013MU02416A (en)
SG (1) SG11201600380VA (en)
WO (1) WO2015008302A1 (en)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4999422A (en) 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
EP1204855A1 (en) * 1999-07-16 2002-05-15 Human Genome Sciences, Inc. Real-time, in situ biomanufacturing process monitoring and control in response to ir spectroscopy
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
DE10022258A1 (en) * 2000-05-08 2001-11-15 Bayer Ag Purification and/or concentration of protein inclusion bodies in solution, by passing the solution tangentially across semipermeable membrane formed from intersecting longitudinal and transverse fibers
GB0116038D0 (en) 2001-06-29 2001-08-22 Middelberg Anton A protein folding reactor
JP2003102494A (en) 2001-07-26 2003-04-08 Sangaku Renkei Kiko Kyushu:Kk Method of refolding protein, continuous protein refolding process, and apparatus therefor
US7060460B2 (en) * 2001-10-03 2006-06-13 Boehringer Ingelheim Austria Gmbh Method for reconstituting a recombinant protein to its biologically active form
JP2004345958A (en) 2003-05-13 2004-12-09 Universal Bio Research Co Ltd Protein refolding device and method of using protein refolding device
WO2007041713A1 (en) * 2005-10-04 2007-04-12 Zymogenetics, Inc. Production and purification of il-29
EP1845103B1 (en) * 2006-04-10 2015-02-25 Boehringer Ingelheim RCV GmbH & Co KG Method for refolding a protein
JP2012520326A (en) * 2009-03-13 2012-09-06 アエロヴァンス インコーポレイティッド Recombinant protein regeneration method
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
WO2014128726A2 (en) * 2013-02-22 2014-08-28 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2014167574A1 (en) * 2013-03-22 2014-10-16 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins

Also Published As

Publication number Publication date
US20160159853A1 (en) 2016-06-09
WO2015008302A1 (en) 2015-01-22
EP3022218A1 (en) 2016-05-25
SG11201600380VA (en) 2016-02-26

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