IN2013MU02416A - - Google Patents

Download PDF

Info

Publication number
IN2013MU02416A
IN2013MU02416A IN2416MU2013A IN2013MU02416A IN 2013MU02416 A IN2013MU02416 A IN 2013MU02416A IN 2416MU2013 A IN2416MU2013 A IN 2416MU2013A IN 2013MU02416 A IN2013MU02416 A IN 2013MU02416A
Authority
IN
India
Prior art keywords
refolding
tank
solubilisation
vessels
feed
Prior art date
Application number
Inventor
Sanjay Sonar
Archana Krishnan
Nikhil Ghade
Faiza Shaikh
Original Assignee
Biogenomics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogenomics Ltd filed Critical Biogenomics Ltd
Priority to SG11201600380VA priority Critical patent/SG11201600380VA/en
Priority to IN2416MU2013 priority patent/IN2013MU02416A/en
Priority to US14/905,438 priority patent/US20160159853A1/en
Priority to PCT/IN2014/000476 priority patent/WO2015008302A1/en
Priority to EP14789629.4A priority patent/EP3022218A1/en
Publication of IN2013MU02416A publication Critical patent/IN2013MU02416A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/24—Stationary reactors without moving elements inside
    • B01J19/245—Stationary reactors without moving elements inside placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/24—Stationary reactors without moving elements inside

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A machine or apparatus for refolding a protein of interest produced recombinantly in a host cell in form of inclusion bodies is provided. The apparatus includes an inclusion bodies (IB) solubilisation tank to solubilise the inclusion bodies; a plurality of refolding vessels or reactors arranged in series to receive a refolding feed; a first tank connected to the IB solubilisation tank to hold diafiltration buffer or agent, at least one diafiltration cartridge, having at least one permeate end and one retentate end, connected to the IB solubilisation tank, through a first retentate end, to feed the refolding feed to the IB solubilisation tank; a second tank, connected to the first permeate end of the diafiltration cartridge to receive and recycle the refolding buffer and each of the refolding vessels, for supplying refolding buffer or agent to each of the refolding vessels.
IN2416MU2013 2013-07-19 2014-07-18 IN2013MU02416A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
SG11201600380VA SG11201600380VA (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18
US14/905,438 US20160159853A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
PCT/IN2014/000476 WO2015008302A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins
EP14789629.4A EP3022218A1 (en) 2013-07-19 2014-07-18 Apparatus for refolding of recombinant proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18

Publications (1)

Publication Number Publication Date
IN2013MU02416A true IN2013MU02416A (en) 2015-06-19

Family

ID=51795686

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2416MU2013 IN2013MU02416A (en) 2013-07-19 2014-07-18

Country Status (5)

Country Link
US (1) US20160159853A1 (en)
EP (1) EP3022218A1 (en)
IN (1) IN2013MU02416A (en)
SG (1) SG11201600380VA (en)
WO (1) WO2015008302A1 (en)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4999422A (en) 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
JP2003504638A (en) * 1999-07-16 2003-02-04 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド Monitoring and control of real-time in-situ biomanufacturing processes in response to infrared spectroscopy
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
DE10022258A1 (en) * 2000-05-08 2001-11-15 Bayer Ag Purification and/or concentration of protein inclusion bodies in solution, by passing the solution tangentially across semipermeable membrane formed from intersecting longitudinal and transverse fibers
GB0116038D0 (en) 2001-06-29 2001-08-22 Middelberg Anton A protein folding reactor
JP2003102494A (en) 2001-07-26 2003-04-08 Sangaku Renkei Kiko Kyushu:Kk Method of refolding protein, continuous protein refolding process, and apparatus therefor
US7060460B2 (en) * 2001-10-03 2006-06-13 Boehringer Ingelheim Austria Gmbh Method for reconstituting a recombinant protein to its biologically active form
JP2004345958A (en) 2003-05-13 2004-12-09 Universal Bio Research Co Ltd Protein refolding system and method for using the same
EP1931704B1 (en) * 2005-10-04 2010-12-15 ZymoGenetics, L.L.C. Production and purification of il-29
EP1845103B1 (en) * 2006-04-10 2015-02-25 Boehringer Ingelheim RCV GmbH & Co KG Method for refolding a protein
WO2010105227A1 (en) * 2009-03-13 2010-09-16 Aerovance, Inc. Methods of renaturation of recombinant proteins
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
US20150376228A1 (en) * 2013-02-22 2015-12-31 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2014167574A1 (en) * 2013-03-22 2014-10-16 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins

Also Published As

Publication number Publication date
WO2015008302A1 (en) 2015-01-22
SG11201600380VA (en) 2016-02-26
US20160159853A1 (en) 2016-06-09
EP3022218A1 (en) 2016-05-25

Similar Documents

Publication Publication Date Title
MY176332A (en) Multispecific antibody constructs
GB2532671A (en) Patient support
EP3274020A4 (en) Pressurized gas powered medicament transfer and re-suspension apparatus and method
WO2017115128A3 (en) Vector-free delivery of gene editing proteins and compositions to cells and tissues
GB2504226A (en) System, apparatus, and method for aligning registers
PL2577923T3 (en) Apparatus, method and computer program product for selecting beam group and subset of beams in communication system
CL2012001539A1 (en) 005-c04 antibody that antagonizes prolactin receptor-mediated signaling; nucleic acid sequence encoding said antibody; expression vector and host cell comprising said nucleic acid sequence; use of the antibody for combination hormonal therapy; pharmaceutical composition comprising the antibody.
MX359235B (en) Method for preparing a composition comprising highly concentrated antibodies by ultrafiltration.
DK2817382T4 (en) COMPOSITION INCLUDING AN ALCOXYLATED AMINE COMPOUND AND A CARBOXYLIC ACID COMPOUND, USING IT IN WATER-IN-OIL EMULSIONS AND PROCEDURE FOR USING THE COMPOSITION SOME COMPOSITION
EP3196289A4 (en) Liquid delivery method for cell culture system, and cell culture system
MX2018000221A (en) Acoustic mixing for auto granulation.
BR112018004474A2 (en) e911 positioning via background signal tracking
BR112016007251A2 (en) Cellulose-containing biomass processing method
BR112017017886A2 (en) generating arterial endothelial cell populations
EP3202885A4 (en) Novel biological activity testing structure for tracking single cell, using gelling agents
CO6551676A2 (en) MODIFIED SWINE SOMATROTROPINE POLYPEPTIDES AND ITS USES
CL2012001183A1 (en) System for loading and unloading in port operations and support of hatch covers of holds of maritime vessels, comprising a loading and unloading crane and a base located in the body of said crane to support and stack said hatch covers; process.
EP3438237A4 (en) Cell culture vessel, support jig for cell culture vessel and cell culture method
BR112017018149A2 (en) recombinant host cell, method for producing a recombinant protein of interest, expression system, and processes for making a stable protein formulation and for reducing esterase activity in a protein formulation.
MX356162B (en) Expression and secretion system.
MY198404A (en) Communication apparatus, control method, and computer readable storage medium
IL273548A (en) Perfusion apparatus for use in bioreactor systems
WO2017075237A3 (en) Chimeric post-transcriptional regulatory element
MY167985A (en) Egg tray advancement system for an in ovo injection apparatus, and associated method
MX2014002606A (en) Apparatus for the passage and conveyance of compressible material.