WO2002092612A3 - Enterotoxin b bindende nukleinsäure - Google Patents

Enterotoxin b bindende nukleinsäure Download PDF

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Publication number
WO2002092612A3
WO2002092612A3 PCT/EP2002/005245 EP0205245W WO02092612A3 WO 2002092612 A3 WO2002092612 A3 WO 2002092612A3 EP 0205245 W EP0205245 W EP 0205245W WO 02092612 A3 WO02092612 A3 WO 02092612A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acids
target molecule
bind
interact
enterotoxin
Prior art date
Application number
PCT/EP2002/005245
Other languages
English (en)
French (fr)
Other versions
WO2002092612A2 (de
Inventor
Susanne Leva
Sven Klussmann
Werner Purschke
Jens Wientges
Original Assignee
Noxxon Pharma Ag
Susanne Leva
Sven Klussmann
Werner Purschke
Jens Wientges
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Noxxon Pharma Ag, Susanne Leva, Sven Klussmann, Werner Purschke, Jens Wientges filed Critical Noxxon Pharma Ag
Priority to AU2002342306A priority Critical patent/AU2002342306A1/en
Publication of WO2002092612A2 publication Critical patent/WO2002092612A2/de
Publication of WO2002092612A3 publication Critical patent/WO2002092612A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

Die vorliegende Erfindung betrifft eine Nukleinsäure bindend an Enterotoxin B von Staphylococcus, insbesondere von Staphylococcus aureus, sowie ein Verfahren zur Herstellung und/oder Identifizierung von Nukleinsäuren, die an ein Zielmolekül binden, insbesondere von einer erfindungsgemässen Nukleinsäure, wobei a) eine heterogene Population von Nukleinsäuren erzeugt wird; b) die Population von Schritt a) in-Kontakt-gebracht wird; c) die Nukleinsäuren, die nicht mit dem Zielmolekül in Wechselwirkung treten abgetrennt werden; d) die Nukleinsäuren, die mit dem Zielmolekül in Wechselwirkung treten optional abgetrennt werden; und e) die Nukleinsäuren, die mit dem Zielmolekül in Wechselwirkung getreten sind optional sequenziert werden. Dadurch gekennzeichnet, dass das Zielmolekül eine Aminosäuresequenz des Enterotoxin B von Staphylococcus, bevorzugterweise von Staphylococcus aureus, umfasst.
PCT/EP2002/005245 2001-05-11 2002-05-13 Enterotoxin b bindende nukleinsäure WO2002092612A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002342306A AU2002342306A1 (en) 2001-05-11 2002-05-13 Nucleic acids that bind to enterotoxin b

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10122847A DE10122847A1 (de) 2001-05-11 2001-05-11 Enterotoxin B bindende Nukleinsäure
DE10122847.3 2001-05-11

Publications (2)

Publication Number Publication Date
WO2002092612A2 WO2002092612A2 (de) 2002-11-21
WO2002092612A3 true WO2002092612A3 (de) 2003-07-10

Family

ID=7684367

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/005245 WO2002092612A2 (de) 2001-05-11 2002-05-13 Enterotoxin b bindende nukleinsäure

Country Status (3)

Country Link
AU (1) AU2002342306A1 (de)
DE (1) DE10122847A1 (de)
WO (1) WO2002092612A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103243101B (zh) * 2013-05-16 2015-01-14 江南大学 一组特异性识别金黄色葡萄球菌肠毒素c1的核酸适配体
CN113667766A (zh) * 2021-07-23 2021-11-19 华南理工大学 产肠毒素b金黄色葡萄球菌的cpa检测引物及其检测试剂盒和方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5475096A (en) * 1990-06-11 1995-12-12 University Research Corporation Nucleic acid ligands
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
WO1998020165A2 (en) * 1996-11-06 1998-05-14 Whitehead Institute For Biomedical Research Biallelic markers
DE19848403A1 (de) * 1998-10-21 2000-04-27 Guenter Von Kiedrowski Verfahren zur exponentiellen Amplifikation molekularer Matrizen
WO2001006249A2 (en) * 1999-07-02 2001-01-25 Conceptual Mindworks, Inc. Organic semiconductor recognition complex and system
DE19952568A1 (de) * 1999-11-02 2001-05-17 Noxxon Pharma Ag Fluoreszenzmarkierte Nukleinsäure-Liganden

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5475096A (en) * 1990-06-11 1995-12-12 University Research Corporation Nucleic acid ligands
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
WO1998020165A2 (en) * 1996-11-06 1998-05-14 Whitehead Institute For Biomedical Research Biallelic markers
DE19848403A1 (de) * 1998-10-21 2000-04-27 Guenter Von Kiedrowski Verfahren zur exponentiellen Amplifikation molekularer Matrizen
WO2001006249A2 (en) * 1999-07-02 2001-01-25 Conceptual Mindworks, Inc. Organic semiconductor recognition complex and system
DE19952568A1 (de) * 1999-11-02 2001-05-17 Noxxon Pharma Ag Fluoreszenzmarkierte Nukleinsäure-Liganden

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BRUNO JOHN G ET AL: "Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods.", BIOTECHNIQUES, vol. 32, no. 1, January 2002 (2002-01-01), pages 178 - 183, XP001119158, ISSN: 0736-6205 *
DATABASE EMBL [online] 1 November 1997 (1997-11-01), XP002222508, Database accession no. AB000712 *
DILLON C A ETAL: "Aptamers as potential bioanalytical recognition elements", SECOND KU UNDERGRADUATE RESEARCH SYMPOSIUM, 1999, XP002221136, Retrieved from the Internet <URL:www.ku.edu/~ugsympos/abstracts1999.html> [retrieved on 20021113] *
KATAHIRA JUN ET AL: "Molecular cloning and functional characterization of the receptor for Clostridium perfringens enterotoxin.", JOURNAL OF CELL BIOLOGY, vol. 136, no. 6, 1997, pages 1239 - 1247, XP002221137, ISSN: 0021-9525 *
LEVA SUSANNE ET AL: "GnRH binding RNA and DNA Spiegelmers: A novel approach toward GnRH antagonism.", CHEMISTRY & BIOLOGY, vol. 9, no. 3, March 2002 (2002-03-01), pages 351 - 359, XP002221140, ISSN: 1074-5521 *
PATEL R M ET AL: "In vitro evolution of RNA-based aptamers to probe toxin active sites.", IJMM INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, vol. 290, no. 4-5, Supplement 30, October 2000 (2000-10-01), 9th European Workshop on Bacterial Protein Toxins;Saint Maxime, France; June 27-July 02, 1999, pages A62, XP001119889, ISSN: 1438-4221 *
TOULMÉ J J: "Aptamers: selected oligonucleotides for therapy.", CURRENT OPINION IN MOLECULAR THERAPEUTICS, vol. 2, no. 3, June 2000 (2000-06-01), pages 318 - 324, XP001119703, ISSN: 1464-8431 *
TRENKLE T ET AL: "Non-stoichiometric reduced complexity probes for cDNA arrays", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 17, September 1998 (1998-09-01), pages 3883 - 3891, XP002122209, ISSN: 0305-1048 *
ZHU C ET AL: "Complete nucleotide sequence and analysis of the locus of enterocyte effacement from rabbit diarrheagenic Escherichia coli RDEC-1.", INFECTION AND IMMUNITY, vol. 69, no. 4, April 2001 (2001-04-01), pages 2107 - 2115, XP002221138, ISSN: 0019-9567 *

Also Published As

Publication number Publication date
DE10122847A1 (de) 2002-11-21
WO2002092612A2 (de) 2002-11-21
AU2002342306A1 (en) 2002-11-25

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