WO2002068459A3 - Non aggregating fluorescent proteins and methods for using the same - Google Patents

Non aggregating fluorescent proteins and methods for using the same Download PDF

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Publication number
WO2002068459A3
WO2002068459A3 PCT/US2002/005749 US0205749W WO02068459A3 WO 2002068459 A3 WO2002068459 A3 WO 2002068459A3 US 0205749 W US0205749 W US 0205749W WO 02068459 A3 WO02068459 A3 WO 02068459A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
subject
protein
nucleic acid
aggregating
Prior art date
Application number
PCT/US2002/005749
Other languages
French (fr)
Other versions
WO2002068459A2 (en
Inventor
Sergey Lukyanov
Konstantin Lukyanov
Yuriy Yanushevich
Alexandr Savistky
Arcady Fradkov
Original Assignee
Clontech Lab Inc
Sergey Lukyanov
Konstantin Lukyanov
Yuriy Yanushevich
Alexandr Savistky
Arcady Fradkov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/006,922 external-priority patent/US7166444B2/en
Application filed by Clontech Lab Inc, Sergey Lukyanov, Konstantin Lukyanov, Yuriy Yanushevich, Alexandr Savistky, Arcady Fradkov filed Critical Clontech Lab Inc
Priority to EP02723238A priority Critical patent/EP1385967A2/en
Priority to JP2002567969A priority patent/JP2004536571A/en
Priority to AU2002254031A priority patent/AU2002254031A1/en
Priority to CA2434737A priority patent/CA2434737C/en
Publication of WO2002068459A2 publication Critical patent/WO2002068459A2/en
Publication of WO2002068459A3 publication Critical patent/WO2002068459A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Nucleic acid compositions encoding non-aggregating chromo/fluoroproteins and mutants thereof, as well as the proteins encoded by the same, are provided. The proteins of interest are polypeptides that are non-aggregating colored and/or fluorescent proteins, where the the non-aggregating feature arises from the modulation of residues in the N-terminus of the protein and the chromo and/or fluorescent feature arises from the interaction of two or more residues of the protein. Also provided are fragments of the subject nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
PCT/US2002/005749 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same WO2002068459A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02723238A EP1385967A2 (en) 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same
JP2002567969A JP2004536571A (en) 2001-02-21 2002-02-20 Non-aggregating fluorescent protein and method of using same
AU2002254031A AU2002254031A1 (en) 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same
CA2434737A CA2434737C (en) 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US27098301P 2001-02-21 2001-02-21
US60/270,983 2001-02-21
US10/006,922 US7166444B2 (en) 1999-10-14 2001-12-04 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US10/006,922 2001-12-04

Publications (2)

Publication Number Publication Date
WO2002068459A2 WO2002068459A2 (en) 2002-09-06
WO2002068459A3 true WO2002068459A3 (en) 2003-11-27

Family

ID=40863845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/005749 WO2002068459A2 (en) 2001-02-21 2002-02-20 Non aggregating fluorescent proteins and methods for using the same

Country Status (5)

Country Link
EP (1) EP1385967A2 (en)
JP (2) JP2004536571A (en)
AU (1) AU2002254031A1 (en)
CA (1) CA2434737C (en)
WO (1) WO2002068459A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1372418A4 (en) * 2001-02-26 2006-01-25 Univ Non-oligomerizing tandem fluorescent proteins
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
DE60233347D1 (en) 2001-12-19 2009-09-24 Univ Chicago FAST-TREATING FLUORESCENT PROTEINS AND METHOD FOR THEIR USE
RU2004118848A (en) * 2001-12-26 2005-04-20 Клонтек Лэборетериз, Инк. (Us) MUTANT CHROMOPHORES / FLUOROPHORES AND METHODS FOR THEIR PRODUCTION AND APPLICATION
WO2004060386A1 (en) * 2002-11-01 2004-07-22 Amgen, Inc. Modulators of receptors for parathyrois hormone and parathyroid hormone-related protein
EP1642969B1 (en) 2003-06-16 2009-09-16 Riken Fluorescent protein and pigment protein
US7250298B2 (en) 2004-04-07 2007-07-31 The University Of Chicago Monomeric red fluorescent proteins
ATE483018T1 (en) 2006-01-25 2010-10-15 Evrogen Ip NEW FLUORESCENT PROTEINS AND METHODS OF USE THEREOF
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
AU2007314614A1 (en) * 2006-04-14 2008-05-08 Celsense, Inc. Methods for assessing cell labeling
CN110226087A (en) * 2017-01-27 2019-09-10 国立大学法人大阪大学 Chemiluminescent indicator used in the detection method of organism substance, this method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027150A2 (en) * 1999-10-14 2001-04-19 Clontech Laboratories Inc. Anthozoa derived chromo/fluoroproteins and methods for using the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027150A2 (en) * 1999-10-14 2001-04-19 Clontech Laboratories Inc. Anthozoa derived chromo/fluoroproteins and methods for using the same

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BAIRD GEOFFREY S ET AL: "Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 97, no. 22, 24 October 2000 (2000-10-24), October 24, 2000, pages 11984 - 11989, XP002208898, ISSN: 0027-8424 *
FRADKOV ARKADY F ET AL: "Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.", FEBS LETTERS, vol. 479, no. 3, 2000, pages 127 - 130, XP002208900, ISSN: 0014-5793 *
MATZ MIKHAIL V ET AL: "Fluorescent proteins from nonbioluminescent Anthozoa species.", NATURE BIOTECHNOLOGY, vol. 17, no. 10, October 1999 (1999-10-01), pages 969 - 973, XP002208899, ISSN: 1087-0156 *
WALL MARK A ET AL: "The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.", NATURE STRUCTURAL BIOLOGY, vol. 7, no. 12, December 2000 (2000-12-01), pages 1133 - 1138, XP001095366, ISSN: 1072-8368 *
WETZEL RONALD: "Mutations and off-pathway aggregation of proteins.", TRENDS IN BIOTECHNOLOGY, vol. 12, no. 5, 1994, pages 193 - 198, XP001093961, ISSN: 0167-9430 *
WIEHLER JENS ET AL: "Mutants of Discosoma red fluorescent protein with a GFP-like chromophore.", FEBS LETTERS, vol. 487, no. 3, 2001, pages 384 - 389, XP002208901, ISSN: 0014-5793 *
YANUSHEVICH YURII G ET AL: "A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.", FEBS LETTERS, vol. 511, no. 1-3, 30 January 2002 (2002-01-30), 30 January, 2002, pages 11 - 14, XP002208902, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
CA2434737A1 (en) 2002-09-06
JP5221308B2 (en) 2013-06-26
CA2434737C (en) 2012-11-06
WO2002068459A2 (en) 2002-09-06
JP2009131260A (en) 2009-06-18
EP1385967A2 (en) 2004-02-04
AU2002254031A1 (en) 2002-09-12
JP2004536571A (en) 2004-12-09

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