WO2002064036A1

WO2002064036A1 - Apparatus and method for collecting residual blood from placenta

Info

Publication number: WO2002064036A1
Application number: PCT/CN2001/000124
Authority: WO
Inventors: Dao-Pei Lu; Yi-Lan Wang; Kai Sun
Original assignee: Dao-Pei Lu; Yi-Lan Wang; Kai Sun
Priority date: 2001-02-13
Filing date: 2001-02-13
Publication date: 2002-08-22
Also published as: CN1489448A

Abstract

A apparatus and method for collecting residual blood from placenta, comprising the following steps: a just expulsed placenta is inserted in a uterus simulative bag having a soft wall, the temperature in the bag is 30 °C - 45 °C; One end of a catheter is inserted in umbilical section veins of the placenta, the other end is connected to a pumpdrainage means, by which the placenta residual blood of is drawn out from the umbilical veins; at the same time, the placenta is intermittently and locally compressed through the uterus simulative bag.

Description

Method and device for collecting placenta residual blood

Field

The invention belongs to the field of clinical umbilical-placental blood hematopoietic stem cell collection, and more particularly, is a method and a collecting device for collecting placental residual blood. Background technique

Hematopoietic stem cell transplantation of bone marrow and peripheral blood has become one of the major advances in the field of hematopoietic stem cell transplantation in the past 10 years. Especially in China, with the increase of single-child families, the proportion of bone marrow donors will decrease. Hematopoietic stem cell transplantation has its unique practical value and shows broad prospects for clinical application. The number of hematopoietic cells in a single cord blood can basically meet the needs of children who undergo stem cell transplantation. However, for adolescents and adult patients who are heavier, the number of hematopoietic cells in a single cord blood has become a major limiting factor. The umbilical cord blood transplantation that has been carried out basically remains in childhood. A certain amount of fetal blood remaining in the placenta is an important supplement to umbilical cord blood, but there is still no satisfactory method for collecting placental residual blood. At present, the two-stage method of cord blood-placental blood collection proposed by Broxmeyer is generally used (see Shen Baijun Et al. "Basics of Human Cord Blood. Clinical", Tianjin Science and Technology Press, 1995, pp. 104-105), the first stage is to collect cord blood from the umbilical cord during the placenta delivery, and the second stage is to puncture multiple times with a syringe needle Placental surface veins are used to obtain the remaining blood in the placenta. However, this method not only collects less placenta remaining blood and contains hematopoietic cells, but also easily causes bacterial and fungal infections. Turner has previously reported the use of catheter collection to collect residual placental blood (see Basics of Human Umbilical Cord Blood. Clinical Science, Tianjin Science and Technology Press, 1995, pp. 105-106). Place the placenta and umbilical cord immediately after delivery by 10%. In the povidone iodine solution, put a sterile umbilical cord cap on the branch of the umbilical blood flow, and immediately cut off the umbilical cord from the end of the umbilical cord cap, and insert the 8-French Feeding Tube into the umbilical cord, In the vein, a closed system is formed. After the umbilical vein blood flow stops, the umbilical artery is injected with saline through the catheter to flush the umbilical blood vessels and placenta, and the umbilical blood is collected from the umbilical vein. However, when the method is repeated according to the literature, it is found that although theoretically speaking This method maximizes Hematopoietic cells are collected, but the umbilical artery is thin and curved, making the intubation operation extremely time-consuming. After the placenta is delivered, the remaining blood in the placenta usually physiologically coagulates within five to ten minutes, especially at low temperatures. The success rate of placenta remaining blood is low; no clinical application has been reported.

Summary of the Invention

The purpose of the present invention is to provide a method for collecting placental residual blood, which can be used to collect more placental residual blood to meet the needs of adolescents and adult patients who are heavier in weight.

Another object of the present invention is to provide a device for collecting placental residual blood, which can implement the above method and collect more placental residual blood to meet the needs of adolescents and adult patients who are heavier in weight.

The invention considers the physiological characteristics of the placenta in the uterus, and realizes a method capable of collecting more placenta remaining blood by simulating the environmental conditions of the placenta in the uterus.

The placenta needs to meet three key environmental conditions in the womb:

(1) Temperature conditions: The placenta is kept in the uterus at the same temperature as the body temperature, and blood flow in the blood vessels in the placenta is smooth. If the temperature is too low, blood may coagulate.

(2) Contraction tension of the uterus: The compression of the placenta by the uterine contraction helps the blood flow in the placenta.

(3) Sterility: When collecting placental residual blood, the collection of placental residual blood should be performed in a sterile environment, which is more complete in terms of aseptic conditions than other placental residual blood collection methods.

The present invention provides a method for collecting placenta residual blood, which includes the following steps: a) Place the placenta that has just been delivered into a uterine simulation bag, the uterine simulation bag has a flexible bag body, and the temperature of the bag body is maintained at 30 ° C--45 ° C;

b) Insert one end of the catheter into the umbilical vein of the umbilical cord section of the placenta. The device is connected, and the placenta remaining blood is extracted from the umbilical vein by using an extraction device; C) while the placental remaining blood is extracted, the placenta is continuously and unevenly squeezed through the bag body of the uterine simulation bag;

This collection method can simulate the environmental conditions of the placenta in the uterus, ensure blood circulation in the placenta, and collect more residual blood from the placenta.

1. Meet the temperature conditions: The temperature of the bag body is maintained at 30. C—45. Between C, the placenta can be kept in the uterus at the same temperature as the body temperature, so that the blood in the placenta will not coagulate.

2. Simulate the tension during uterine contraction: While extracting the remaining blood from the placenta, the bag body through the uterine simulation bag is continuously and non-uniformly squeezed or called intermittent partial compression of the placenta, which can simulate the uterine contraction. tension. If uniform pressure, such as air pressure, is used to press the placenta, the uniform hook tension acting on the placenta is not conducive to the collection of remaining blood in the placenta, because the uniform pressure causes the umbilical vein and the circulation path to be squeezed and blocked simultaneously, and the placenta cannot be left. Blood pressure is out. The use of uterine contraction to squeeze the placenta during delivery can increase the collection yield. Therefore, this method, while extracting the remaining blood from the placenta, squeezes the placenta through the bag of the uterine simulation bag continuously and non-uniformly.

3. Aseptic collection: The placenta is enclosed in the bag with the help of a uterine simulation bag, which is more exposed to the air than other placenta collection methods, and its aseptic conditions are more complete. Moreover, with the protection of the bag body, it is avoided to directly squeeze the placenta, which is also beneficial for aseptic collection.

The method for collecting placental residual blood of the present invention can be further developed:

The uterine simulation bag can be set as follows: the uterine simulation bag is a silent layer bag made of a flexible material; the flexible material is rubber, plastic or plastic. Further, an opening is formed on the outer layer of the double-layer bag, and the opening is reversibly sealed, such as plugging or clamping with a clip; or the opening edge of the uterine simulation bag is set as a closing structure. The uterine simulation bag is also set up as follows: the opening side of the uterine simulation bag is provided with an extended closed edge or an openable structure, and the bag body is provided with an opening to facilitate the umbilical cord on the placenta when the placenta is inserted into the bag body. Stick out from it.

In the collecting method of the present invention, the temperature of the uterine simulation bag is filled with 30 in the interlayer of the double-layer bag. C—45. C's liquid hold. The liquid is physiological saline or sterilized purified water. The liquid can be injected in a variety of ways. This is known to those skilled in the art. The present invention does not limit the method of liquid injection.

In the collecting method of the present invention, the extraction device connected to the catheter is a syringe. Further, the catheter connected between the syringe and the umbilical vein is provided with a three-way joint, the third port of the three-way joint is connected with a blood bag, and the three-way joint is provided with a transfer switch; A changeover switch will drain the placental blood drawn from the umbilical vein into the syringe into the iliac blood bag. The transfer switch can be implemented in various ways. For example, a section of the transfer switch is a round pipe, and a plane formed by three ports of the vertical tee joint can be rotatably extended into the tee joint, and the wall of the round pipe There are at least two notches or holes. The other section of the rotary switch is a handle. Turn the handle to change the alignment direction of the notches or holes on the wall of the circular tube, and connect two ports of the tee joint. In the collecting method of the present invention, the extraction device connected to the duct may also be an air pump provided with an air inlet and an air outlet. The air inlet of the air exhaust pump is provided with an air inlet check valve. The air port is provided with an air outlet check valve, the air inlet is connected to a catheter connected to the umbilical vein, and a blood collection bag is connected to the air outlet. Because placenta usually solidifies in about 5-10 minutes after delivery, especially at low temperatures. In order to improve the speed of anticoagulation and blood collection, an extraction device was used to connect the iliac vein and iliac blood bag on the placenta through a catheter during the collection of placental blood. Therefore, it is ensured that the blood is aseptically collected into the blood collection bag containing anticoagulant within 5 minutes after the placenta is delivered.

In the collecting method of the present invention, the temperature of the bag body is preferably maintained at 36 °. C—42. Between C. The temperature of the bag body is preferably 40 ° C.

In the collecting method of the present invention, the catheter inserted into the umbilical vein of the umbilical section of the placenta The catheter wall of the port is provided with at least one gap to form a split structure to prevent the port of the catheter from being blocked by the umbilical vein wall. It can also be set as follows: at least one opening is provided in the catheter wall near the port of the catheter inserted in the umbilical vein of the umbilical section of the placenta to prevent the port of the catheter from being blocked by the umbilical vein wall.

In the collecting method of the present invention, the catheter inserted into the umbilical vein of the umbilical section of the placenta is double-layered at the portion near the port or a section of the catheter from the portion near the port, and the outer layer of the catheter near the port is partially provided It is a thin wall, and an air inlet is provided on the outer layer of the catheter. When the gas is filled into the interlayer of the catheter wall from the air inlet, the local thin wall swells to form a gas sacral, which tightly combines the outer layer of the catheter with the umbilical vein. At the same time, the inner layer of the catheter is not excessively compressed. When collecting placental blood, the catheter is very easy to slide in the umbilical vein at the base of the placenta. If it is pressurized and fixed from the outside, the catheter will be deformed and blood will be blocked, which will affect blood collection. Therefore, a double-layered catheter is inserted into the confluence of the umbilical vein at the base of the placenta, and then the gas is filled with gas. The purpose of fixing the catheter and blocking the umbilical artery blood flow is achieved, and the placental base The umbilical vein confluence often collapses due to negative pressure suction blood collection, blocking the venous blood return, and setting the catheter before the urinary catheter is stent-like or openings in the front section of the catheter, which significantly improves the phenomenon of venous blood return obstruction. .

According to the method for collecting the remaining blood of the placenta,

The invention also provides a collection device for placenta residual blood, which is provided with: a uterine simulation bag, the uterine simulation bag is a flexible bag body, and the temperature of the bag body is maintained at 30. C- -45. Between C

The extraction device is connected with a catheter which can be inserted into the umbilical vein of the umbilical cord section of the placenta.

The collecting device of the present invention can be further developed:

The uterine simulation bag in the acquisition device of the present invention may be set as follows: The uterine simulation bag is a double-layer bag made of a flexible material. Further, the flexible material is rubber, plastic or plastic. The double-layer bag has an opening on the outer layer, and is reversibly sealed on the opening, such as a plug, Or clamp it with a clip. The interlayer of the double-layer bag body is filled with 30 ° C- -45. C of liquid. The liquid is physiological saline or purified water after disinfection.

The uterine simulation bag in the acquisition device of the present invention may also be set as follows: The opening edge of the uterine simulation bag is set as a closing structure. Alternatively, the opening edge of the uterine simulation bag is provided with an extended closing edge or an openable structure, and the bag body is further provided with an opening from which the umbilical cord on the placenta protrudes when the placenta is inserted into the bag body.

In the collecting device of the present invention, the extraction device may be a syringe, and a tee joint is provided on a catheter connected between the syringe and the umbilical vein, and a blood collection bag is connected to a third port of the tee joint, The three-way joint is provided with a changeover switch for controlling the conduction of the port; a section of the changeover switch is set as a round pipe, and a plane formed by three ports of the vertical three-way joint rotatably extends into the three-way joint, and the wall of the round pipe There are at least two notches or holes on the top, and the other section of the rotary switch is a handle. Turning the handle changes the alignment direction of the notches or holes on the wall of the circular tube, and connects two ends α of the three-way joint.

In the collecting device of the present invention, the extraction device may also be an air pump provided with an air inlet and an air outlet. The air inlet of the air exhaust pump is provided with an air inlet check valve, and the air outlet is provided with an air outlet. The inlet valve is connected to a catheter connected to the umbilical vein, and a blood collection bag is connected to the outlet.

In the acquisition device of the present invention, at least one notch is provided in the catheter wall of the port of the umbilical vein inserted into the umbilical vein section of the placenta to form a split structure to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

In the acquisition device of the present invention, at least one opening is provided on a catheter wall of the umbilical vein of the umbilical vein inserted into the umbilical cross section of the placenta to prevent the port of the catheter from being blocked by the umbilical vein wall.

In the collecting device of the present invention, the catheter inserted into the umbilical vein of the umbilical cord section of the placenta is double-layered at the portion near the port or a section of the catheter from the portion near the port, and the outer layer of the catheter is provided with an inlet. The air outlet, the outer layer of the duct close to the port is partially thin-walled and guided from the air inlet When the interlayer of the tube wall is filled with gas, the local thin wall bulges to tightly bond the outer layer of the catheter with the umbilical vein while not compressing the inner layer of the catheter excessively.

Brief description of the drawings

1 is a flowchart of a method for collecting placental residual blood according to the present invention;

Figure 2 is a schematic diagram of a section of the umbilical cord;

3 is a first schematic structural diagram of a placenta residual blood collection device according to the present invention;

4 is a schematic diagram of a second structure of a placenta residual blood collection device according to the present invention;

5 is a schematic diagram of a first structure of a uterine simulation bag of a placenta residual blood collection device according to the present invention;

6 is a schematic diagram of a second structure of a uterine simulation bag of the placenta residual blood collection device of the present invention;

7 is a sectional view of a catheter of a placenta residual blood collection device of the present invention;

8 is an inhalation state of a first structure of a tee joint of a placenta residual blood collection device of the present invention;

9 is a state of discharge of a first structure of a tee joint of a placenta residual blood collection device of the present invention;

10 is a sealed state of a tee joint of the placenta residual blood collection device of the present invention;

FIG. 11 is a suction state of the two structures of the tee joint of the placenta residual blood collection device of the present invention; FIG.

12 is a discharge state of the second structure of the tee joint of the placenta residual blood collection device of the present invention;

13 is a closed state of the second structure of the tee joint of the placenta residual blood collection device of the present invention; FIG. 14 is a schematic structural diagram of an air pump as an extraction device of the placenta residual blood collection device of the present invention. Embodiment 1 of Method for Collecting Remaining Blood of Placenta

The method for collecting placenta remaining blood according to the present invention is a method for further collecting blood remaining inside the placenta after the collection of conventional cord blood. In the collecting method of the present invention, the operation should be performed under sterile conditions, and during the operation, it can be sterilized by conventional disinfection methods, such as ethylene oxide sterilization and disinfection. At the time of delivery, after the collection of umbilical cord blood, the distal end of the umbilical cord is clamped with sterile hemostatic forceps. After the placenta is delivered, the remaining blood of the placenta is collected. The method includes the following steps:

a) Place the placenta that has just been delivered into the uterine simulation bag 1. The uterine simulation bag 1 has a flexible bag body, and the temperature of the bag body is controlled at 30. C—45. C; wherein, the uterine simulation bag 1 may be a double-layer bag made of a flexible material. The flexible material may be a material such as rubber, plastic, or plastic. For example, a polyvinyl chloride plastic used for preparing a blood collection bag may be selected. The temperature of the uterine simulation bag is filled with 30 in the interlayer of the double bag. C-45. C's liquid remains. The outer layer 12 of the double-layer bag has an opening, and a plug 16 is plugged into the opening. A liquid 14 of a certain temperature is injected from the opening 16 of the outer layer 12 to keep the uterine simulation bag 1 at a certain temperature. The liquid may be physiological saline or pure water after disinfection, as shown in FIG. 5.

b) Insert one end 26 of the catheter 2 into the umbilical vein V of the umbilical cord section of the placenta. As shown in the schematic diagram of the end face of the umbilical cord, a is the umbilical artery. The other end 25 of the catheter 1 is connected to an extraction device 3, and the placental blood is withdrawn from the umbilical vein V by the extraction device 3;

c) while the remaining blood of the placenta is drawn out, the bag body through the uterine simulation bag is continuously and non-uniformly squeezed (or called intermittent partial squeezed placenta);

Further, the opening edge of the uterine simulation bag is set as a closing structure. The closing structure preferably has elasticity. When the placenta is placed in the uterine simulation bag, only the umbilical cord protrudes from the opening. The closing structure closes the placenta in the bag body and isolates it from the outside world to make the aseptic condition more perfect. This can also be done: As shown in FIG. 5, the opening 13 of the uterine simulation bag is provided with an extended closed edge 131 or an openable structure. The openable structure is provided with a convex edge 132 on the inner wall of one side of the opening, corresponding to one side. A groove 133 is provided on the inner wall, and the convex edge 132 can be snapped into or pulled out from the groove 133 so as to be opened. The bag body is also provided with an opening 15 so that when the placenta is inserted into the bag body, the umbilical cord on the placenta protrudes therefrom. With this structure, the placenta is enclosed in the bag body and isolated from the outside world, so that the aseptic conditions are more perfect.

Because the remaining blood in the placenta is limited, when the volume of the extraction device is large, most of the placenta can be left with a single inhalation of the extraction device, and then the blood in the extraction device is injected into the blood collection bag. While injecting blood into the blood collection bag, use another extraction device to continue to extract the remaining blood from the placenta's umbilical vein. Alternatively, the extraction device may be connected to the blood collection bag through a catheter, and the blood is repeatedly extracted from the placenta through a one-way switching channel, and discharged into the blood collection bag.

During the collection process, the extraction device 3 connected to the catheter may be a syringe, as shown in FIG. 3. The catheter 2 connected between the syringe and the umbilical vein V is provided with a three-way joint 5, the third port 55 of the three-way joint 5 is connected to a blood collection bag 4, and the three-way joint 5 is provided with a transfer switch; The change-over switch 52 on the connector 5 discharges the placental blood drawn from the umbilical vein V into the syringe into the blood collection bag 4. The 52-section of the transfer switch is a round pipe, and a plane formed by three ports of the vertical tee joint rotatably extends into the tee joint 5. The wall of the round pipe is provided with at least two notches or holes to rotate. The other part of the switch is the handle 56. Turn the handle to change the alignment of the notches or holes on the wall of the tube, and connect two ports of the tee joint. There are various implementations of the three-way connector. As long as a transfer port can be connected to only one of the other two ports in different states through a changeover switch. The present invention provides two embodiments for illustration. 8 to FIG. 13, wherein FIG. 8 to FIG. 10 are the first structure. 8 is an inhalation state of a first structure of a tee joint of a placental residual blood collection device of the present invention; FIG. 9 is an exhaust state of a first structure of a tee joint of a placental residual blood collection device of the present invention; FIG. 10 is a closed state of a first structure of a tee joint of a placenta residual blood collection device of the present invention. The three The through joint is provided with a housing 51, and three ports 511, 512, and 513 are connected to the housing. A changeover switch 52 is provided in the housing, and the changeover switch is provided with three openings 521, 522, and 523. The changeover switch 52 can be rotated in the housing by turning the handle 56 connected to the changeover switch to change the alignment relationship between the opening on the changeover switch and the port on the housing to achieve the placenta drawn from the umbilical vein V into the syringe Blood is drained into the blood collection bag 4. Example setting: Port 511 of the three-way connector is connected to a syringe, port 512 is connected to a blood collection bag, and port 513 is connected to a catheter for extracting placental blood from the umbilical vein. As shown in FIG. 8, the opening 521 is aligned with the port 511, and the opening 523 is aligned with the port 513, so that the port 511 of the tee communicates with the port 513, the port 512 and the ports 511 and 513 are cut off, and placental blood can flow in from the port 513 511, Placental blood is drawn into the syringe. As shown in FIG. 9, the opening 522 is aligned with the port 511, and the opening 521 is aligned with the port 512, so that the port 511 of the tee is in communication with the port 512, the port 513 and ports 511 and 512 are cut off, and placental blood can flow from the port 511 512, Placental blood is drained from the syringe. As shown in FIG. 10, each opening of the transfer switch is not aligned with any port on the housing, and the three-way joint is in a cut-off state. 11 to 13 show a second structure of the three-way joint. 11 is an inhalation state of the second structure of the tee joint of the placental residual blood collection device of the present invention; FIG. 12 is an exhaust state of the second structure of the tee joint of the placental residual blood collection device of the present invention; FIG. 13 is a closed state of the second structure of the tee joint of the placenta residual blood collection device of the present invention. The tee joint is provided with a housing 51, and three ports 511, 512, and 513 are connected to the housing. A changeover switch 52 is provided in the casing, and the changeover switch is provided with two openings 521 and 522. The switch 56 can be rotated in the housing by turning the handle 56 connected to the switch to change the alignment relationship between the opening on the switch and the port on the housing, so as to achieve the Placental blood is drained into the blood collection bag 4. Example setting: Port 512 of the three-way connector is connected to a syringe, port 511 is connected to a blood collection bag, and port 513 is connected to a catheter for extracting placental blood from the umbilical vein. As shown in FIG. 11, the opening 521 is aligned with the port 512, and the opening 522 is aligned with the port 513, so that the port 512 of the tee is in communication with the port 513, the port 511 and ports 512 and 513 are cut off, and placental blood can flow in from the port 513 512, inhaled Placental blood to the syringe. As shown in FIG. 12, the opening 522 is aligned with the port 512, and the opening 521 is aligned with the port 511, so that the port 511 of the tee is in communication with the port 512, the port 513 and ports 511 and 512 are cut off, and placental blood can flow from the port 512 511. The placental blood is discharged from the syringe to the blood collection bag. As shown in FIG. 13, each opening of the transfer switch is not aligned with any port on the housing, and the three-way joint is in a cut-off state.

During the collection process, the temperature of the uterine simulation bag is controlled between 30 ° C and 45 ° C, and the temperature of the bag body is preferably maintained at 36 ° C. C-42. Between C, the optimal bag temperature is 40 ° C. C, can be controlled by the temperature of the liquid filled in the bag sandwich.

The bag body intermittently extruding the placenta through the uterine simulation bag is an extrusion that simulates the uterine contraction tension, which is helpful for the collection of placental blood.

As shown in FIG. Ί, the catheter wall of the port 26 of the umbilical vein inserted into the umbilical vein section of the placenta is provided with at least one gap 23 to form a split structure to prevent the port of the catheter from being blocked by the umbilical vein wall.

As shown in FIG. 7, at least one opening 22 is provided on the catheter wall near the end of the catheter in the umbilical vein inserted into the umbilical section of the placenta to prevent the port of the catheter from being blocked by the umbilical vein wall.

As shown in FIG. 7, the portion of the catheter 1 inserted into the umbilical vein of the umbilical section of the placenta near the port 26 or a section of the catheter from the portion near the port 26 is set as a double layer 241, 242 near the port 26 The outer layer 241 of the duct 2 is partially set as a thin wall 243. The outer layer 241 of the duct 2 is provided with an air inlet 21, and an air inlet check valve is provided at the air inlet 21. As shown in the figure, it may be an elastomer. 212 structure, the center of the elastic body is provided with a vent hole 211, the vent hole is wedge-shaped, the outer end becomes larger, the inner end becomes smaller, and the inner end is closed and abutted; when inflated, the tip port of the air needle or the injection tip port of the syringe passes from the vent The air hole 211 is squeezed in, inflated into the interlayer, and the thin wall 243 is bulged. While maintaining the high pressure in the interlayer, the air needle or syringe is pulled out. At this time, the gas does not leak out, and the thin wall 243 remains bulged. status. When filling the interlayer of the duct wall from the air inlet 21, the thin wall 243 is partially Bulging, tightly bonding the outer layer 241 of the catheter with the umbilical vein v, while not compressing the inner layer 242 of the catheter excessively. Example 2 of a method for collecting placenta residual blood

Refer to FIG. 4, which is a schematic diagram of a second structure of the placenta residual blood collection device of the present invention. The steps of this embodiment and the collection method of the above embodiment are basically the same, except that the structure of the bag body of the uterine simulation bag and the structure of the extraction device are different. At the same time, the tee joint is eliminated, and the blood collection bag is directly connected. On the air pump as a suction device.

As shown in FIG. 6, the uterine simulation bag 1 has a flexible bag body, and the temperature of the bag body is controlled at 30. C—45. Between C; wherein, the uterine simulation bag 1 may be a Han-layer bag made of a flexible material, with an inner layer of 11 and an outer layer of 12. The flexible material may be a material such as rubber, plastic, or plastic. For example, a polyvinyl chloride plastic used for preparing a blood collection bag may be selected. The temperature of the uterine simulation bag is maintained by providing a heating device 16 or a heating device in the interlayer of the silent bag, such as an electric heating wire 16, or other methods known to those skilled in the art.

Please refer to FIG. 14, the extraction device 3 connected to the duct is an air pump provided with an air inlet 32 and an air outlet 33, and the air inlet 32 of the air exhaust pump is provided with an air inlet check valve 321 The air outlet 33 is provided with an air outlet check valve 331, the air inlet 32 is connected to one end 25 of the catheter 2 connected to the umbilical vein, and the air outlet 33 is connected to the blood collection bag 4. The air pump is provided with a pump body 31, and a piston 34 is provided in the pump body. A push rod 35 is connected to the piston 34, and the push rod 35 extends from an end of the pump body opposite to the air inlet and the air outlet. During operation, the push rod 35 is pulled to move the piston 34 outward, the check valve 331 in the air outlet 33 is closed, the check valve 321 in the air inlet 32 is opened, and placental blood flows from the air inlet 32 connected to the catheter 2 Then, push the push rod 35 to move the piston 34 inward, the one-way valve 331 in the air outlet 33 is opened, the one-way valve 321 in the air inlet 32 is closed, and the placental blood is connected to the blood collection bag 4 The air outlet 33 flows into the blood collection bag. Repeatedly, the blood in the placenta is continuously drawn into the blood collection bag. Example 1 of placenta residual blood collection device

A collection device for placenta residual blood, the collection device is provided with:

A uterine simulation bag, which is a flexible bag body, which is provided with a temperature control device; an extraction device, which is connected with a catheter that can be inserted into the umbilical vein of the umbilical cord section of the placenta.

The uterine simulation bag 1 may be a Han bag made of a flexible material. The flexible material may be a material such as rubber, plastic, or plastic, such as a polyvinyl chloride plastic used for preparing a blood collection bag. The temperature control device of the uterine simulation bag can be a liquid maintained at a certain temperature in the interlayer of the silent bag body and the double-layer bag body, that is, the temperature of the uterine simulation bag is filled with the interlayer of the double-layer bag.

30 ° C—45 ° C liquid hold. An opening is formed on the outer layer 12 of the double-layer bag, and a plug 16 is plugged on the opening. A liquid 14 of a certain temperature is injected from the opening 16 of the outer layer 12 to keep the uterine simulation bag 1 at a certain temperature. The liquid may be physiological saline or sterilized pure water, as shown in FIG. 5.

Insert one end 26 of the catheter 2 into the umbilical vein V of the umbilical cord section of the placenta, as shown in the schematic diagram of the end face of the umbilical cord, a is the iliac artery. The other end 25 of the catheter 1 is connected to an extraction device 3, and the placental blood is withdrawn from the umbilical vein V by the extraction device 3;

Further, the opening edge of the uterine simulation bag is set as a closing structure. The closing structure preferably has elasticity. When the placenta is placed in the uterine simulation bag, only the umbilical cord protrudes from the opening. The closing structure closes the placenta in the bag body and isolates it from the outside world to make the aseptic condition more perfect. Alternatively, as shown in FIG. 5, the opening 13 of the uterine simulation bag is provided with an extended closed edge 131 or an openable structure, and the openable structure is provided with a convex edge 132 on the inner wall of one side of the opening. A groove 133 is provided on the inner wall of the corresponding side, and the convex edge 132 can be snapped into or removed from the groove 133 to achieve unsealing. The bag body is also provided with an opening 15 so that when the placenta is inserted into the bag body, the umbilical cord on the placenta protrudes from it. With this structure, the placenta is enclosed in the bag body and isolated from the outside world. Make the aseptic conditions more perfect.

Because the remaining blood in the placenta is limited, when the volume of the extraction device is large, most of the placental blood can be left with a single inhalation of the extraction device, and then the blood in the extraction device is injected into the blood collection device. Keep in the bag; while injecting blood into the blood collection bag, use another extraction device to continue to draw the remaining blood from the placenta's umbilical vein. Alternatively, the extraction device may be connected to the blood collection bag through a catheter.

The extraction device 3 connected to the catheter may be a syringe, as shown in FIG. 3. The catheter 2 connected between the syringe and the umbilical vein V is provided with a three-way joint 5. The third port 55 of the three-way joint 5 is connected with a blood collection bag 4, and the three-way joint 5 is provided with a transfer switch. The changeover switch 52 on the through connector 5 discharges the placental blood drawn from the umbilical vein V into the syringe into the blood collection bag 4. The section of the changeover switch 52 is a round pipe, and a plane formed by three ports of the vertical tee joint rotatably extends into the tee joint 5. The wall of the round pipe is provided with at least two notches or holes to rotate. The other part of the switch is the handle 56. Turn the handle to change the alignment of the notches or holes on the wall of the tube, and connect two ports of the tee joint. There are various implementations of the three-way connector. As long as a transfer port can be connected to only one of the other two ports in different states through a changeover switch. The present invention provides two embodiments for illustration. 8 to FIG. 13, wherein FIG. 8 to FIG. 10 are the first structure. 8 is an inhalation state of a first structure of a tee joint of a placental residual blood collection device of the present invention; FIG. 9 is an exhaust state of a first structure of a tee joint of a placental residual blood collection device of the present invention; FIG. 10 is a closed state of a first structure of a tee joint of a placenta residual blood collection device of the present invention. The tee joint is provided with a housing 51, and three ports 511, 512, and 513 are connected to the housing. A changeover switch 52 is provided in the housing, and the changeover switch is provided with three openings 521, 522, and 523. The changeover switch 52 can be rotated in the housing by turning the handle 56 connected to the changeover switch to change the alignment relationship between the opening on the changeover switch and the port on the housing to achieve the placenta drawn from the umbilical vein V into the syringe. Blood is drained into the blood collection bag 4. Example setting: port 511 of the three-way connector is connected to the syringe, port 512 Connected to a blood collection bag, port 513 is connected to a catheter for extracting placental blood from the umbilical vein. As shown in FIG. 8, the opening 521 is aligned with the port 511, and the opening 523 is aligned with the port 513, so that the port 511 of the tee communicates with the port 513, the port 512 and the ports 511 and 513 are cut off, and placental blood can flow in from the port 513 511. Placental blood is drawn into the syringe. As shown in FIG. 9, the opening 522 is aligned with the port 511, and the opening 521 is aligned with the port 512, so that the port 511 of the tee is in communication with the port 512, the port 513 and ports 511 and 512 are both cut off, and placental blood can flow from the port 511 512, Placental blood is drained from the syringe. As shown in FIG. 10, each opening of the transfer switch is misaligned with any port on the housing, and the tee joint is in a cut-off state. 11 to 13 show a second structure of the three-way joint. 11 is an inhalation state of the second structure of the tee joint of the placental residual blood collection device of the present invention; FIG. 12 is an exhaust state of the second structure of the tee joint of the placental residual blood collection device of the present invention; FIG. 13 is a closed state of the second structure of the tee joint of the placenta residual blood collection device of the present invention. The three-way joint is provided with a housing 51, and three ports 511, 512, and 513 are connected to the housing. A changeover switch 52 is provided in the casing, and the changeover switch is provided with two openings 521 and 522. By turning the handle 56 connected to the transfer switch, the transfer switch 52 can be rotated in the housing, and the alignment relationship between the opening on the transfer switch and the port on the housing can be changed, so as to achieve the Placental blood is drained into the blood collection bag 4. Example setting: Port 512 of the three-way connector is connected to a syringe, port 511 is connected to a blood collection bag, and port 513 is connected to a catheter for extracting placental blood from the umbilical vein. As shown in FIG. 11, the opening 521 is aligned with the port 512, and the opening 522 is aligned with the port 513, so that the port 512 of the tee is in communication with the port 513, the ports 511 and 512 and 513 are cut off, and placental blood can flow in from the port 513 512. Inhale the placental blood into the syringe. As shown in FIG. 12, the opening 522 is aligned with the port 512, and the opening 521 is aligned with the port 511, so that the port 511 of the tee is in communication with the port 512, the ports 513 and 511 and 512 are cut off, and placental blood can flow from the port 512 511. The placental blood is drained from the syringe to the blood collection bag. As shown in FIG. 13, each opening of the transfer switch is not aligned with any port on the housing, and the three-way joint is in a cut-off state. As shown in Fig. ，, the catheter wall of the port 26 of the catheter 2 in the venous vein of the umbilical section of the placenta is provided with at least one gap 23 to form a split structure to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

As shown in FIG. 7, the portion of the catheter 2 inserted into the umbilical vein of the umbilical cord section of the placenta near the port 26 or a section of the catheter from the portion near the port 26 is set to the Chinese layer 241, 242, close to the port 26 The outer layer 241 of the duct 2 is partially set as a thin wall 243. The outer layer 241 of the duct 2 is provided with an air inlet 21, and an air inlet check valve is provided at the air inlet 21. As shown in the figure, it can be an elastomer. 212 structure, the center of the elastic body is provided with a vent hole 211, the vent hole is wedge-shaped, the outer end is gradually larger, the inner end is gradually smaller, and the innermost end is closed and abutted; The air hole 211 is squeezed in, inflated into the interlayer, and the thin wall 243 is bulged. While maintaining the high pressure in the interlayer, the air needle or syringe is pulled out. At this time, the gas does not leak out, and the thin wall 243 remains bulged. status. When the gas is filled from the air inlet 21 to the interlayer of the catheter wall, the local thin wall 243 swells, so that the outer layer 241 of the catheter is tightly combined with the umbilical vein V, and the inner layer 242 of the catheter is not excessively compressed, see FIG. 2 Show. Example 2 of placenta residual blood collection device

Refer to FIG. 4, which is a schematic diagram of a second structure of the placenta residual blood collection device of the present invention. The structure of this embodiment is basically the same as the collection device of the above embodiment, except that the structure of the bag body of the uterine simulation bag and the structure of the extraction device are different. At the same time, the tee joint is eliminated, and the blood collection bag is directly connected. On the air pump as a suction device.

As shown in FIG. 6, the uterine simulation bag 1 has a flexible bag body, and the temperature of the bag body is controlled at

30. C—45. C; wherein, the uterine simulation bag 1 may be a silent layer made of a flexible material The bag has an inner layer of 11 and an outer layer of 12. The flexible material may be a material such as rubber, plastic, or plastic. For example, a polyvinyl chloride plastic used for preparing a blood collection bag may be selected. The temperature of the uterine simulation bag is maintained by providing a heating device 17 or a heating device in the interlayer of the double-layer bag, such as a heating wire 17, or other methods known to those skilled in the art.

Please refer to FIG. 14, the extraction device 3 connected to the duct is an air pump provided with an air inlet 32 and an air outlet 33, and the air inlet 32 of the air exhaust pump is provided with an air inlet check valve 321 The air outlet 33 is provided with an air outlet check valve 331, the air inlet 32 is connected to one end 25 of the catheter 1 connected to the umbilical vein, and the air outlet 33 is connected to the blood collection bag 4. The air pump is provided with a pump body 31, a piston 34 is provided in the pump body, and a push rod 35 is connected to the piston 34, and the push rod 35 protrudes from an end of the pump body opposite to the air inlet and the air outlet. During operation, the push rod 35 is pulled to move the piston 34 outward, the check valve 331 in the air outlet 33 is closed, the check valve 321 in the air inlet 32 is opened, and placental blood flows from the air inlet 32 connected to the catheter 2 Then, push the push rod 35 to move the piston 34 inward, the one-way valve 331 in the air outlet 33 is opened, the one-way valve 321 in the air inlet 32 is closed, and the placental blood is connected to the blood collection bag 4 The air outlet 33 flows into the blood collection bag. Repeatedly, the blood in the placenta is continuously drawn into the blood collection bag.

Claims

Claim

1. A method for collecting placental residual blood, the method comprising the following steps:

a) Place the placenta that has just been delivered into a uterine simulation bag. The uterine simulation bag has a flexible bag body, and the temperature of the bag body is maintained at 30. C—45. Between C

b) Insert one end of the catheter into the umbilical vein of the umbilical cord section of the placenta, and connect the other end of the catheter to an extraction device, and use the extraction device to extract the remaining blood from the placenta from the umbilical vein;

c) While the remaining blood of the placenta is drawn out, the placenta is continuously and unevenly squeezed through the bag body of the uterine simulation bag.

2. The method for collecting residual blood of the placenta according to claim 1, wherein the uterine simulation bag is a silent layer bag made of a flexible material.

3. The method for collecting residual blood of the placenta according to claim 2, wherein the flexible material is rubber, plastic or plastic.

4. The method for collecting residual blood of the placenta according to claim 2, characterized in that: the outer layer of the double-layer bag has an opening, and the opening is reversibly sealed.

5. The method for collecting residual placental blood according to claim 2, characterized in that: the temperature of the uterine simulation bag is filled with 30 in the interlayer of the double-layer bag. C- -45. C's liquid remains.

6. The method for collecting blood remaining in the placenta according to claim 5, wherein the liquid is physiological saline or sterilized purified water.

7. The method for collecting residual blood of the placenta according to claim 1, wherein: the opening edge of the uterine simulation bag is set as a closing structure.

8. The method of collecting blood remaining in the placenta according to claim 1, characterized in that: the opening edge of the uterine simulation bag is provided with an extended closed edge or an openable structure, and the bag body is provided with another There is an opening for the umbilical cord on the placenta to protrude from the placenta when it is placed in the bag.

9. The method for collecting blood from the placenta as claimed in claim 1, wherein: The extraction device connected to the catheter is a syringe.

10. The method for collecting placenta residual blood according to claim 9, characterized in that: the catheter connected between the syringe and the umbilical vein is provided with a tee joint, and the third port of the tee joint A blood collection bag is connected, and a transfer switch is provided on the tee joint; the transfer switch on the transfer tee joint discharges the placental blood drawn from the umbilical vein into the syringe into the blood collection bag.

11. A method for collecting placental residual blood according to claim 10, characterized in that: one section of the changeover switch is a round tube, and a plane formed by three ports of a vertical tee joint is rotatably extended. Into the tee joint, at least two notches or holes are provided on the wall of the round pipe. The other part of the rotary switch is a handle. Turn the handle to change the alignment direction of the notches or holes on the round pipe wall. Two of them.

12. A method for collecting placenta residual blood according to claim 1, wherein: the extraction device connected to the catheter is an air pump provided with an air inlet and an air outlet, and the air exhaust pump The air inlet is provided with an air inlet check valve, and the air outlet is provided with a air outlet check valve. The air inlet is connected to a catheter connected to the umbilical vein, and the air outlet is connected with a blood collection bag.

13. The method for collecting residual blood of a placenta according to claim 1, wherein the temperature of the bag body is maintained between 36 ° C and 42 ° C.

14. The method for collecting blood remaining in the placenta according to claim 1, wherein the temperature of the bag body is preferably 40. C.

15. The method for collecting residual blood of the placenta according to claim 1, wherein the kneading of the placenta through the bag body of the uterine simulation bag is a squeeze that simulates the uterine contraction tension.

16. The method for collecting placenta residual blood according to claim 1, characterized in that: the catheter wall of the port of the catheter inserted into the umbilical vein of the umbilical section of the placenta is provided with at least one gap to form an opening Fork structure to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

17. The method for collecting placenta residual blood according to claim 1, characterized in that: the catheter wall of the umbilical vein inserted into the umbilical vein of the umbilical section of the placenta is near the port wall of the catheter. At least one opening is provided to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

18. The method for collecting residual blood of the placenta according to claim 1, characterized in that: the catheter inserted into the umbilical vein of the umbilical section of the placenta is near the port or from a position near the port A section of the duct is set to the Chinese layer, and the outer layer of the duct close to the port is partially thin-walled, and an air inlet is provided on the outer layer of the duct. When the gas is filled from the air inlet to the interlayer of the duct wall, the local thin wall swells. The outer layer of the catheter is tightly combined with the umbilical vein, while the inner layer of the catheter is not excessively compressed.

19. A method for collecting placental residual blood, the method comprising the following steps:

a) The placenta that has just been delivered is placed in a uterine simulation bag. The uterine simulation bag is a silent bag made of a flexible material, and 30 is filled in the interlayer of the double bag. C--45. C liquid; b) insert one end of the catheter into the umbilical vein of the umbilical section of the placenta, the other end of the catheter is connected to a syringe, and the remaining blood in the placenta is extracted from the umbilical vein by using the syringe; the connection is between the syringe and the umbilical vein The catheter is provided with a three-way joint, the third port of the three-way joint is connected with a blood collection bag, and the three-way joint is provided with a transfer switch; the transfer switch on the three-way joint is rotated to draw the placental blood from the umbilical vein into the syringe. Drain into a blood collection bag.

c) While removing the remaining blood from the placenta, rub the placenta through the bag body of the uterine simulation bag.

20. A collection device for placenta residual blood, characterized in that the collection device is provided with:

Uterine simulation bag, which is a flexible bag body, and the temperature of the bag body is maintained at

30. C- 45 ° C;

The extraction device is connected with a catheter that can be inserted into the umbilical vein of the umbilical cord section of the placenta.

21. The device for collecting placental residual blood according to claim 20, wherein the uterine simulation bag is a double-layer bag made of a flexible material.

22. The device for collecting residual blood of the placenta according to claim 21, wherein the flexible material is rubber, plastic or plastic.

23. The device for collecting residual blood of the placenta according to claim 21, wherein an outer layer of the double-layer bag has an opening, and a plug is plugged in the opening.

24. The device for collecting residual blood of the placenta according to claim 21, wherein: the interlayer of the double-layer bag body is filled with 30. C-45. C of liquid.

25. The device for collecting residual blood of the placenta according to claim 24, wherein the liquid is physiological saline or sterilized pure water.

26. The device for collecting residual blood of the placenta according to claim 20, characterized in that: the opening edge of the uterine simulation bag is set as a closing structure.

27. The device for collecting residual blood of the placenta according to claim 20, characterized in that: the opening edge of the uterine simulation bag is provided with an extended closed edge or an openable structure, and the bag body is further provided with An opening through which the umbilical cord on the placenta projects when the placenta is placed into the bag.

28. The device for collecting placenta residual blood according to claim 20, wherein the extraction device is a syringe.

29. The device for collecting residual blood of the placenta according to claim 28, characterized in that: the catheter connected between the syringe and the umbilical vein is provided with a tee joint, and the third port of the tee joint A blood collection bag is connected, and a transfer switch for controlling the conduction of the port is provided on the three-way connector.

30. The device for collecting residual blood of the placenta according to claim 29, wherein a section of the changeover switch is a round tube, and a plane formed by three ports of the vertical tee joint is rotatably extended. Into the tee joint, at least two notches or holes are provided on the wall of the round pipe. The other part of the rotary switch is a handle. Turn the handle to change the alignment direction of the notches or holes on the round pipe wall. Two of them.

31. The device for collecting placenta residual blood according to claim 20, wherein the extraction device connected to the catheter is an air pump provided with an air inlet and an air outlet, and the air exhaust pump The air inlet is provided with an air inlet check valve, and the air outlet is provided with a air outlet check valve. The air inlet is connected to a catheter connected to the umbilical vein, and the air outlet is connected with a blood collection bag.

32. The device for collecting residual blood of the placenta according to claim 20, characterized in that: the catheter wall of the port of the catheter inserted into the umbilical vein of the umbilical section of the placenta is provided with at least one gap to form an opening Fork structure to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

33. The device for collecting residual blood of the placenta according to claim 20, characterized in that: at least one opening is provided on the wall of the catheter near the port of the catheter inserted into the umbilical vein of the umbilical section of the placenta. Hole to prevent the port of the catheter from being blocked by the wall of the umbilical vein.

34. The device for collecting residual blood of the placenta according to claim 20, characterized in that: the catheter inserted into the umbilical vein of the umbilical section of the placenta is near the port or from a position near the port A section of the duct is double-layered, and the outer layer of the duct is provided with an air inlet. The outer layer of the duct close to the port is partially thin-walled. When the gas is filled from the air inlet to the interlayer of the duct wall, the thin-walled part bulges. In order not to compress the inner layer of the catheter excessively, the outer layer of the catheter is tightly combined with the umbilical vein.