WO2002061143A3 - Comparative analysis of nucleic acids using population tagging - Google Patents

Comparative analysis of nucleic acids using population tagging Download PDF

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Publication number
WO2002061143A3
WO2002061143A3 PCT/US2002/003097 US0203097W WO02061143A3 WO 2002061143 A3 WO2002061143 A3 WO 2002061143A3 US 0203097 W US0203097 W US 0203097W WO 02061143 A3 WO02061143 A3 WO 02061143A3
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WO
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Application
Patent type
Prior art keywords
nucleic acids
tagging
population
samples
nucleic acid
Prior art date
Application number
PCT/US2002/003097
Other languages
French (fr)
Other versions
WO2002061143A2 (en )
Inventor
David Brown
Matthew M Winkler
Original Assignee
Ambion Inc
David Brown
Matthew M Winkler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

Abstract

Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.
PCT/US2002/003097 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging WO2002061143A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US26569401 true 2001-01-31 2001-01-31
US60/265,694 2001-01-31

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20020720894 EP1360333A2 (en) 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging
US10632534 US20040058373A1 (en) 2001-01-31 2003-07-31 Competitive amplification of fractionated targets from multiple nucleic acid samples
US10632539 US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10632539 Continuation US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging

Publications (2)

Publication Number Publication Date
WO2002061143A2 true WO2002061143A2 (en) 2002-08-08
WO2002061143A3 true true WO2002061143A3 (en) 2003-03-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003097 WO2002061143A3 (en) 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging

Country Status (2)

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EP (1) EP1360333A2 (en)
WO (1) WO2002061143A3 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017186A3 (en) * 2003-08-08 2005-05-06 Univ Jefferson Method for rapid identification of alternative splicing
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
EP1791982B1 (en) 2004-09-21 2011-01-05 Life Technologies Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (MIRNAs)
WO2007117256A1 (en) * 2005-05-31 2007-10-18 Applera Corporation Multiplexed amplification of short nucleic acids
DK2463386T3 (en) 2005-06-15 2017-07-31 Complete Genomics Inc Nucleic acid analysis using the random mixtures of non-overlapping fragments
ES2490601T3 (en) * 2006-11-15 2014-09-04 Biospherex Llc multi-tag sequencing and analysis ecogenómico
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US20140243242A1 (en) * 2011-08-08 2014-08-28 The Broad Institute. Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (en) * 1989-09-06 1991-03-13 Zeneca Limited Amplification processes
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
EP0870842A2 (en) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation Adaptor-tagged competitive PCR

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (en) * 1989-09-06 1991-03-13 Zeneca Limited Amplification processes
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
EP0870842A2 (en) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation Adaptor-tagged competitive PCR

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US9441267B2 (en) 1999-04-20 2016-09-13 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Also Published As

Publication number Publication date Type
EP1360333A2 (en) 2003-11-12 application
WO2002061143A2 (en) 2002-08-08 application

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