WO2002061041A3 - Solubility reporter gene constructs - Google Patents

Solubility reporter gene constructs Download PDF

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Publication number
WO2002061041A3
WO2002061041A3 PCT/US2001/051426 US0151426W WO02061041A3 WO 2002061041 A3 WO2002061041 A3 WO 2002061041A3 US 0151426 W US0151426 W US 0151426W WO 02061041 A3 WO02061041 A3 WO 02061041A3
Authority
WO
WIPO (PCT)
Prior art keywords
solubility
target protein
protein
polynucleotides
identify
Prior art date
Application number
PCT/US2001/051426
Other languages
French (fr)
Other versions
WO2002061041A2 (en
WO2002061041A9 (en
Inventor
Scott Lesley
Mark Knuth
Original Assignee
Irm Llc
Scott Lesley
Mark Knuth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Irm Llc, Scott Lesley, Mark Knuth filed Critical Irm Llc
Priority to EP01998134A priority Critical patent/EP1364055A4/en
Priority to CA002428248A priority patent/CA2428248A1/en
Priority to JP2002561598A priority patent/JP2004535772A/en
Publication of WO2002061041A2 publication Critical patent/WO2002061041A2/en
Publication of WO2002061041A3 publication Critical patent/WO2002061041A3/en
Publication of WO2002061041A9 publication Critical patent/WO2002061041A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides polynucleotides that a protein solubility response promoter operatively liked to a reporter gene and a genetic reporter system comprising these polynucleotides together with an expression construct for a target protein. The invention also provides cells comprising polynucleotides of the invention and the genetic reporter system. These compositions are useful to monitor the solubility of a target protein in a cell and to identify mutations to the cell or mutations to a polynucleotide encoding the target protein that alters the solubility of the target protein. The invention further provides method to identify variations in a protein biosynthetic process that alter the solubility of a target protein and methods to screen an expression library of recombinant clones to identify clones that express soluble proteins. Finally, the invention discloses a novel method of identifying an antibiotic agent.
PCT/US2001/051426 2000-11-21 2001-11-21 Solubility reporter gene constructs WO2002061041A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01998134A EP1364055A4 (en) 2000-11-21 2001-11-21 Solubility reporter gene constructs
CA002428248A CA2428248A1 (en) 2000-11-21 2001-11-21 Solubility reporter gene constructs
JP2002561598A JP2004535772A (en) 2000-11-21 2001-11-21 Soluble reporter gene structure

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US72134000A 2000-11-21 2000-11-21
US09/721,340 2000-11-21
US32483301P 2001-09-24 2001-09-24
US60/324,833 2001-09-24

Publications (3)

Publication Number Publication Date
WO2002061041A2 WO2002061041A2 (en) 2002-08-08
WO2002061041A3 true WO2002061041A3 (en) 2003-09-04
WO2002061041A9 WO2002061041A9 (en) 2004-05-13

Family

ID=26984650

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/051426 WO2002061041A2 (en) 2000-11-21 2001-11-21 Solubility reporter gene constructs

Country Status (4)

Country Link
EP (1) EP1364055A4 (en)
JP (1) JP2004535772A (en)
CA (1) CA2428248A1 (en)
WO (1) WO2002061041A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016062819A1 (en) * 2014-10-22 2016-04-28 Danmarks Tekniske Universitet A two-cassette reporter system for assessing target gene translation and target gene product inclusion body formation
CN109055417B (en) * 2018-08-28 2020-07-07 浙江新和成股份有限公司 Recombinant microorganism, preparation method thereof and application thereof in production of coenzyme Q10

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589337A (en) * 1992-07-06 1996-12-31 The President And Fellows Of Harvard College Methods and diagnostic kits for determining toxicity utilizing bacterial stress promoters fused to reporter genes
US5731163A (en) * 1994-11-23 1998-03-24 E. I. Du Pont De Nemours And Company Lyophilized bioluminescent bacterial reagent for the detection of toxicants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589337A (en) * 1992-07-06 1996-12-31 The President And Fellows Of Harvard College Methods and diagnostic kits for determining toxicity utilizing bacterial stress promoters fused to reporter genes
US5731163A (en) * 1994-11-23 1998-03-24 E. I. Du Pont De Nemours And Company Lyophilized bioluminescent bacterial reagent for the detection of toxicants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LESLEY ET AL.: "Gene expression response to misfolded protein as a screen for soluble recombinant protein", PROTEIN ENG., vol. 15, no. 2, 2002, pages 153 - 160, XP002962868 *
PARSELL ET AL.: "Induction of a heat shock-like response by unfolded protein in escherichia coli: dependence on protein level not protein degradation", GENES DEV., vol. 3, August 1989 (1989-08-01), pages 1226 - 1232, XP002962866 *
See also references of EP1364055A4 *
TROTTER ET AL.: "Misfolded proteins are competent to mediate a subset of the responses to heat shock in saccharomyces cerevisiae", J. BIOL. CHEM., vol. 277, no. 47, 22 November 2002 (2002-11-22), pages 44817 - 44825, XP002962867 *
WIGLEY ET AL.: "Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein", NAT. BIOTECHNOL., vol. 19, 19 February 2001 (2001-02-19), pages 131 - 136, XP002962869 *

Also Published As

Publication number Publication date
WO2002061041A2 (en) 2002-08-08
CA2428248A1 (en) 2002-08-08
EP1364055A2 (en) 2003-11-26
WO2002061041A9 (en) 2004-05-13
JP2004535772A (en) 2004-12-02
EP1364055A4 (en) 2004-08-25

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