WO2002052035A3 - Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants - Google Patents

Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants Download PDF

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Publication number
WO2002052035A3
WO2002052035A3 PCT/US2001/046134 US0146134W WO02052035A3 WO 2002052035 A3 WO2002052035 A3 WO 2002052035A3 US 0146134 W US0146134 W US 0146134W WO 02052035 A3 WO02052035 A3 WO 02052035A3
Authority
WO
WIPO (PCT)
Prior art keywords
cad
identification
methods
alcohol dehydrogenase
cinnamyl alcohol
Prior art date
Application number
PCT/US2001/046134
Other languages
French (fr)
Other versions
WO2002052035A2 (en
Inventor
Carol M Hamilton
Robert A Ascenzi
Neil E Hoffman
Keith R Davis
Adel Zayed
Jorn Gorlach
Douglas C Boyes
Jeffrey P Woessner
Andreas S Kloti
Original Assignee
Paradigm Genetics Inc
Carol M Hamilton
Robert A Ascenzi
Neil E Hoffman
Keith R Davis
Adel Zayed
Jorn Gorlach
Douglas C Boyes
Jeffrey P Woessner
Andreas S Kloti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc, Carol M Hamilton, Robert A Ascenzi, Neil E Hoffman, Keith R Davis, Adel Zayed, Jorn Gorlach, Douglas C Boyes, Jeffrey P Woessner, Andreas S Kloti filed Critical Paradigm Genetics Inc
Priority to AU2002241575A priority Critical patent/AU2002241575A1/en
Publication of WO2002052035A2 publication Critical patent/WO2002052035A2/en
Publication of WO2002052035A3 publication Critical patent/WO2002052035A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present inventors have discovered that cinnamyl alcohol dehydrogenase (CAD) is essential for plant growth. Specifically, the inhibition CAD gene expression in plant seedlings results in severe chlorosis and reduced growth. Thus, CAD can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit CAD expression or activity, comprising: contacting a compound with a CAD and detecting the presence and/or absence of binding between said compound and said a CAD, or detecting a decrease in CAD expression or activity. The methods of the invention are useful for the identification of herbicides.
PCT/US2001/046134 2000-10-30 2001-10-30 Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants WO2002052035A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002241575A AU2002241575A1 (en) 2000-10-30 2001-10-30 Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69983400A 2000-10-30 2000-10-30
US09/699,834 2000-10-30

Publications (2)

Publication Number Publication Date
WO2002052035A2 WO2002052035A2 (en) 2002-07-04
WO2002052035A3 true WO2002052035A3 (en) 2003-11-06

Family

ID=24811115

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046134 WO2002052035A2 (en) 2000-10-30 2001-10-30 Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants

Country Status (2)

Country Link
AU (1) AU2002241575A1 (en)
WO (1) WO2002052035A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2392444A (en) * 2002-08-29 2004-03-03 Syngenta Participations Ag Nucleic acid molecules encoding proteins essential for plant growth and development and uses thereof
US6921643B2 (en) 2003-02-05 2005-07-26 The Regents Of The University Of California Compositions and methods for detecting a sequence mutation in the cinnamyl alcohol dehydrogenase gene associated with altered lignification in loblolly pine
CA2768794C (en) 2009-07-22 2018-09-18 E. I. Du Pont De Nemours And Company Sequences and their use for detection and characterization of e. coli o157:h7

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US53782A (en) * 1866-04-10 Improvement in nut-machines
US4978686A (en) * 1987-04-13 1990-12-18 Kiyoshi Sotome Method of protecting crops by a non-toxic composition
FR2697133A1 (en) * 1992-10-28 1994-04-29 Transbiotech Synergistic biocidal and biostatic compsns, active against eg bacteria, fungi and viruses - contg a sesquiterpene, eg pinene, and an aromatic cpd, eg carvacrol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US53782A (en) * 1866-04-10 Improvement in nut-machines
US4978686A (en) * 1987-04-13 1990-12-18 Kiyoshi Sotome Method of protecting crops by a non-toxic composition
FR2697133A1 (en) * 1992-10-28 1994-04-29 Transbiotech Synergistic biocidal and biostatic compsns, active against eg bacteria, fungi and viruses - contg a sesquiterpene, eg pinene, and an aromatic cpd, eg carvacrol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RALPH, J. ET AL: "Abnormal lignin in a loblolly pine mutant", SCIENCE, vol. 277, 11 July 1997 (1997-07-11), pages 235 - 239, XP002245403 *

Also Published As

Publication number Publication date
AU2002241575A1 (en) 2002-07-08
WO2002052035A2 (en) 2002-07-04

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