WO2002097040A3 - Methods for the identification of inhibitors of pectin esterase expression or activity in plants - Google Patents

Methods for the identification of inhibitors of pectin esterase expression or activity in plants Download PDF

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Publication number
WO2002097040A3
WO2002097040A3 PCT/US2002/016648 US0216648W WO02097040A3 WO 2002097040 A3 WO2002097040 A3 WO 2002097040A3 US 0216648 W US0216648 W US 0216648W WO 02097040 A3 WO02097040 A3 WO 02097040A3
Authority
WO
WIPO (PCT)
Prior art keywords
identification
pectin esterase
activity
pectin
methods
Prior art date
Application number
PCT/US2002/016648
Other languages
French (fr)
Other versions
WO2002097040A2 (en
Inventor
Adel Zayed
Robert Ascenzi
Douglas Boyes
Rao Mulpuri
Neil Hoffman
Keith Davis
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Kenneth Phillips
Original Assignee
Paradigm Genetics Inc
Adel Zayed
Robert Ascenzi
Douglas Boyes
Rao Mulpuri
Neil Hoffman
Keith Davis
Jorn Gorlach
Jeffrey Woessner
Carol Hamilton
Kenneth Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc, Adel Zayed, Robert Ascenzi, Douglas Boyes, Rao Mulpuri, Neil Hoffman, Keith Davis, Jorn Gorlach, Jeffrey Woessner, Carol Hamilton, Kenneth Phillips filed Critical Paradigm Genetics Inc
Priority to EP02749538A priority Critical patent/EP1390484A4/en
Priority to AU2002320039A priority patent/AU2002320039A1/en
Publication of WO2002097040A2 publication Critical patent/WO2002097040A2/en
Publication of WO2002097040A3 publication Critical patent/WO2002097040A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present inventors have discovered that pectin esterase is essential for plant growth. Specifically, the inhibition of pectin esterase gene expression resulted in seedlings that were damaged, short, and stunted at a very early growth stage. Thus, pectin esterase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit pectin esterase expression or activity, comprising: contacting a compound with a pectin esterase and detecting the presence and/or absence of binding between said compound and said pectin esterase, or detecting a decrease in pectin .esterase expression or activity. The methods of the invention are useful for the identification of herbicides.
PCT/US2002/016648 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants WO2002097040A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02749538A EP1390484A4 (en) 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants
AU2002320039A AU2002320039A1 (en) 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29448701P 2001-05-30 2001-05-30
US60/294,487 2001-05-30

Publications (2)

Publication Number Publication Date
WO2002097040A2 WO2002097040A2 (en) 2002-12-05
WO2002097040A3 true WO2002097040A3 (en) 2003-10-02

Family

ID=23133648

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016648 WO2002097040A2 (en) 2001-05-30 2002-05-29 Methods for the identification of inhibitors of pectin esterase expression or activity in plants

Country Status (3)

Country Link
EP (1) EP1390484A4 (en)
AU (1) AU2002320039A1 (en)
WO (1) WO2002097040A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5298397A (en) * 1989-10-19 1994-03-29 Yamasa Shoyu Kabushiki Kaisha Method of assaying d-vanillylmandelic acid
US5659121A (en) * 1991-12-20 1997-08-19 Zeneca Limited DNA, DNA constructs, cells and plants derived therefrom
US5910632A (en) * 1994-02-23 1999-06-08 Zeneca Limited DNA encoding a pectin esterase, cells and plants derived therefrom
US6069000A (en) * 1996-02-21 2000-05-30 Novo Nordisk A/S Enzyme with pectin esterase activity
US6271033B1 (en) * 1986-11-11 2001-08-07 Zeneca Limited Method for modifying production of fruit ripening enzyme

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5296376A (en) * 1986-11-04 1994-03-22 Imperial Chemical Industries Plc DNA, constructs, cells and plants derived therefrom
EP0580252A2 (en) * 1992-07-20 1994-01-26 Quest International B.V. Improvements in or relating to pectin methyl esterase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271033B1 (en) * 1986-11-11 2001-08-07 Zeneca Limited Method for modifying production of fruit ripening enzyme
US5298397A (en) * 1989-10-19 1994-03-29 Yamasa Shoyu Kabushiki Kaisha Method of assaying d-vanillylmandelic acid
US5659121A (en) * 1991-12-20 1997-08-19 Zeneca Limited DNA, DNA constructs, cells and plants derived therefrom
US5910632A (en) * 1994-02-23 1999-06-08 Zeneca Limited DNA encoding a pectin esterase, cells and plants derived therefrom
US6069000A (en) * 1996-02-21 2000-05-30 Novo Nordisk A/S Enzyme with pectin esterase activity

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GOUBET F. ET AL.: "Identification and partial characterization of the pectin methyltransferase "homogalactouronan-methyltransferase" from membranes of Tobacco cell suspensions", PLANT PHYSIOLOGY, vol. 116, January 1998 (1998-01-01), pages 337 - 347, XP002960317 *
See also references of EP1390484A4 *
SHINMYO A. ET AL.: "Metabolic engineering of cultured Tobacco cells", BIOTECHNOLOGY AND BIOENGINEERING, vol. 58, no. 2 AND 3, May 1998 (1998-05-01), pages 329 - 332, XP002963002 *

Also Published As

Publication number Publication date
WO2002097040A2 (en) 2002-12-05
AU2002320039A1 (en) 2002-12-09
EP1390484A2 (en) 2004-02-25
EP1390484A4 (en) 2005-01-26

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