WO2002045495A3 - Souris transgeniques contenant disruptions geniques ciblees - Google Patents

Souris transgeniques contenant disruptions geniques ciblees Download PDF

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Publication number
WO2002045495A3
WO2002045495A3 PCT/US2001/046864 US0146864W WO0245495A3 WO 2002045495 A3 WO2002045495 A3 WO 2002045495A3 US 0146864 W US0146864 W US 0146864W WO 0245495 A3 WO0245495 A3 WO 0245495A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
transgenic mice
targeted gene
mice containing
gene disruptions
Prior art date
Application number
PCT/US2001/046864
Other languages
English (en)
Other versions
WO2002045495A2 (fr
Inventor
Keith D Allen
Michael W Leviten
Robert Klein
Original Assignee
Deltagen Inc
Keith D Allen
Michael W Leviten
Robert Klein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen Inc, Keith D Allen, Michael W Leviten, Robert Klein filed Critical Deltagen Inc
Priority to AU2002239539A priority Critical patent/AU2002239539A1/en
Publication of WO2002045495A2 publication Critical patent/WO2002045495A2/fr
Publication of WO2002045495A3 publication Critical patent/WO2002045495A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0356—Animal model for processes and diseases of the central nervous system, e.g. stress, learning, schizophrenia, pain, epilepsy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des animaux transgéniques, ainsi que des compositions et des méthodes afférentes à la caractérisation de la fonction génique. Notamment, cette invention a trait à des souris transgéniques comprenant des mutations au niveau d'un gène similaire à CHD2, d'un gène TDCDF1, d'un gène TR2, d'un gène similaire à E3, d'un gène CSPI et d'un gène Abcd2. On utilise de telles souris transgéniques en tant que modèles de maladies et d'agents d'identification qui modulent l'expression génique et la fonction génique, et en tant que traitements potentiels de maladies et de troubles.
PCT/US2001/046864 2000-12-06 2001-12-05 Souris transgeniques contenant disruptions geniques ciblees WO2002045495A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002239539A AU2002239539A1 (en) 2000-12-06 2001-12-05 Transgenic mice containing targeted gene disruptions

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US25181900P 2000-12-06 2000-12-06
US60/251,819 2000-12-06
US25431400P 2000-12-08 2000-12-08
US60/254,314 2000-12-08
US25597200P 2000-12-13 2000-12-13
US25598300P 2000-12-13 2000-12-13
US25623200P 2000-12-13 2000-12-13
US25596800P 2000-12-13 2000-12-13
US60/256,232 2000-12-13
US60/255,983 2000-12-13
US60/255,972 2000-12-13
US60/255,968 2000-12-13

Publications (2)

Publication Number Publication Date
WO2002045495A2 WO2002045495A2 (fr) 2002-06-13
WO2002045495A3 true WO2002045495A3 (fr) 2004-02-12

Family

ID=27559388

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046864 WO2002045495A2 (fr) 2000-12-06 2001-12-05 Souris transgeniques contenant disruptions geniques ciblees

Country Status (2)

Country Link
AU (1) AU2002239539A1 (fr)
WO (1) WO2002045495A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002310256A1 (en) * 2001-06-05 2002-12-16 Exelixis Inc. Ppp2cs as modifiers of the p53 pathway and methods of use
US20100031378A1 (en) 2008-08-04 2010-02-04 Edwards Joel A Novel gene disruptions, compositions and methods relating thereto
ZA200606461B (en) * 2004-02-12 2008-02-27 Lexicon Pharmaceuticals Inc Gene disruptions, compositions and methods relating thereto
EP1863340A2 (fr) * 2005-03-11 2007-12-12 Genentech, Inc. Disruptions geniques, compositions et methodes afferentes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043783A2 (fr) * 1998-02-25 1999-09-02 The Scripps Research Institute SOURIS TRANSGENIQUE A GENE MARQUEUR LacZ REGULE PAR LE PROMOTEUR N-CAM SERVANT A L'IDENTIFICATION DE MODULATEURS N-CAM, METHODES ET COMPOSITIONS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043783A2 (fr) * 1998-02-25 1999-09-02 The Scripps Research Institute SOURIS TRANSGENIQUE A GENE MARQUEUR LacZ REGULE PAR LE PROMOTEUR N-CAM SERVANT A L'IDENTIFICATION DE MODULATEURS N-CAM, METHODES ET COMPOSITIONS

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AGARWALA KISHAN LAL; NAKAMURA SAWAKO; TSUTSUMI YUKIE; YAMAKAWA KAZUHIRO: "Down syndrome cell adhesion molecule DSCAM mediates homophilic intercellular adhesion.", MOLECULAR BRAIN RESEARCH, vol. 79, no. 1-2, 23 June 2000 (2000-06-23), pages 118 - 126, XP002251256 *
CAPECCHI M R: "TARGETED GENE REPLACEMENT", SCIENTIFIC AMERICAN, SCIENTIFIC AMERICAN INC. NEW YORK, US, March 1994 (1994-03-01), pages 52 - 59, XP002940139, ISSN: 0036-8733 *
MANSOUR S L ET AL: "DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM CELLS: A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 336, 24 November 1988 (1988-11-24), pages 348 - 352, XP002052220, ISSN: 0028-0836 *
YAMAKAWA K ET AL: "DSCAM: a novel member of the immunoglobulin superfamily maps in a Down syndrome region and is involved in the development of the nervous system", HUMAN MOLECULAR GENETICS, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 7, no. 2, 1998, pages 227 - 237, XP002959189, ISSN: 0964-6906 *

Also Published As

Publication number Publication date
AU2002239539A1 (en) 2002-06-18
WO2002045495A2 (fr) 2002-06-13

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