WO2002042498A3 - Analyses a l'aide de complexe a deux billes, biodisques optiques, et procedes d'analyse - Google Patents

Analyses a l'aide de complexe a deux billes, biodisques optiques, et procedes d'analyse Download PDF

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Publication number
WO2002042498A3
WO2002042498A3 PCT/US2001/044679 US0144679W WO0242498A3 WO 2002042498 A3 WO2002042498 A3 WO 2002042498A3 US 0144679 W US0144679 W US 0144679W WO 0242498 A3 WO0242498 A3 WO 0242498A3
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Prior art keywords
dual bead
capture
bead complex
dual
target agent
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PCT/US2001/044679
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English (en)
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WO2002042498A2 (fr
Inventor
Brigitte C Phan
Jorma A Virtanen
Amethyst H Lam
Shih-Li Lo
James H Coombs
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Burstein Technologies Inc
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Application filed by Burstein Technologies Inc filed Critical Burstein Technologies Inc
Priority to AU2002216743A priority Critical patent/AU2002216743A1/en
Publication of WO2002042498A2 publication Critical patent/WO2002042498A2/fr
Publication of WO2002042498A3 publication Critical patent/WO2002042498A3/fr

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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
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  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne une méthode permettant de déterminer si un agent cible est présent dans un échantillon biologique. Ladite méthode consiste à mélanger des billes de capture magnétique, une sonde de transport étant fixée sur chacune d'elles, des billes reporter, une sonde de signal étant également fixée sur chacune d'elle, et un échantillon biologique dans des conditions de liaison permettant la formation d'un complexe à deux billes si ledit agent cible est présent dans ledit échantillon, ledit complexe à deux billes comprenant la bille reporter liée à l'agent cible qui à son tour est lié à la bille de capture magnétique; à isoler le complexe à deux billes du mélange par soumission de ce mélange à un champ magnétique; à charger l'isolat obtenu sur un disque optique doté d'une couche de capture dans un champ de capture, un agent de capture se liant spécifiquement au complexe à deux billes; et à détecter la présence du complexe à deux billes sur le disque optique, ce qui indique que l'agent cible est présent dans l'échantillon.
PCT/US2001/044679 2000-11-27 2001-11-27 Analyses a l'aide de complexe a deux billes, biodisques optiques, et procedes d'analyse WO2002042498A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002216743A AU2002216743A1 (en) 2000-11-27 2001-11-27 Dual bead assays including optical biodiscs and methods relating thereto

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US25328300P 2000-11-27 2000-11-27
US60/253,283 2000-11-27
US25395800P 2000-11-28 2000-11-28
US60/253,958 2000-11-28
US27252501P 2001-03-01 2001-03-01
US60/272,525 2001-03-01

Publications (2)

Publication Number Publication Date
WO2002042498A2 WO2002042498A2 (fr) 2002-05-30
WO2002042498A3 true WO2002042498A3 (fr) 2003-07-31

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PCT/US2001/044679 WO2002042498A2 (fr) 2000-11-27 2001-11-27 Analyses a l'aide de complexe a deux billes, biodisques optiques, et procedes d'analyse

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AU (1) AU2002216743A1 (fr)
WO (1) WO2002042498A2 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9418981D0 (en) 1994-09-21 1994-11-09 Univ Glasgow Apparatus and method for carrying out analysis of samples
US6327031B1 (en) 1998-09-18 2001-12-04 Burstein Technologies, Inc. Apparatus and semi-reflective optical system for carrying out analysis of samples
US7026131B2 (en) 2000-11-17 2006-04-11 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-discs
US7087203B2 (en) 2000-11-17 2006-08-08 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-disc
US7079468B2 (en) 2000-12-08 2006-07-18 Burstein Technologies, Inc. Optical discs for measuring analytes
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
US7091034B2 (en) 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
WO2002068696A2 (fr) * 2001-02-27 2002-09-06 Burstein Technologies, Inc. Procedes de conjugaison d'adn sur une phase solide y compris biodisques optiques et systemes d'entrainement de disque connexes
WO2002068697A2 (fr) * 2001-02-28 2002-09-06 Burstein Technologies, Inc. Procedes visant a reduire les liaisons non specifiques de billes dans des techniques a deux billes incluant des biodisques optiques et des systemes d'entrainement de disques associes
WO2002073605A2 (fr) * 2001-03-14 2002-09-19 Burstein Technologies, Inc. Essai biologique a deux perles utilisant des espaceurs clivables et/ou la ligature pour ameliorer la specificite et la sensibilite, procedes et dispositifs associes
US7141416B2 (en) 2001-07-12 2006-11-28 Burstein Technologies, Inc. Multi-purpose optical analysis optical bio-disc for conducting assays and various reporting agents for use therewith
EP1470424A2 (fr) 2002-01-31 2004-10-27 Burstein Technologies, Inc. Procede de declenchement par le biais de rainures de disque, et systeme et disques d'analyse optique associes
AUPS159702A0 (en) 2002-04-09 2002-05-16 Tong, Sun Wing Molecular detection and assay by magneto-thermal biochip micro-assay
US20040018495A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
US20070231796A1 (en) 2003-09-17 2007-10-04 The Regents Of The University Of California Sensor and method for detection of a target substance
US20060040273A1 (en) * 2004-08-17 2006-02-23 Alison Chaiken Method and apparatus for magnetic sensing and control of reagents
WO2006035392A1 (fr) * 2004-09-27 2006-04-06 Koninklijke Philips Electronics N. V. Biocapteurs utilises pour analyser des echantillons
US8296088B2 (en) 2006-06-02 2012-10-23 Luminex Corporation Systems and methods for performing measurements of one or more materials
EP2765423B1 (fr) * 2006-06-02 2016-11-02 Luminex Corporation Systèmes et procédé pour réaliser des mesures sur un ou plusieurs matériaux
KR101343034B1 (ko) 2006-09-05 2013-12-18 삼성전자 주식회사 원심력 기반의 단백질 검출용 미세유동 장치 및 이를포함하는 미세유동 시스템
US7951333B2 (en) 2006-09-05 2011-05-31 Samsung Electronics Co., Ltd. Centrifugal force-based microfluidic device for protein detection and microfluidic system including the same
ES2304093A1 (es) * 2006-12-11 2008-09-01 Universidad Politecnica De Valencia Sistema integrado, procedimiento y disco de almacenamiento de datos para analisis quimico de muestras y para detectar y almacenar los resultados analiticos.
CN102007416B (zh) * 2008-02-14 2013-10-16 三星电子株式会社 生物盘片、生物盘片读取器及使用生物盘片的分析方法
TW201113523A (en) * 2009-08-31 2011-04-16 Mbio Diagnostics Inc Integrated sample preparation and analyte detection
CN102253193A (zh) * 2010-05-20 2011-11-23 上海医脉赛科技有限公司 一种快速检测微生物的磁性荧光试剂盒及其制备方法和使用方法
US9512468B2 (en) * 2012-11-06 2016-12-06 Industrial Technology Research Institute Detection method uses magnetic and detectable nanoparticles with oligonucleotides attached thereto
CN105087353A (zh) * 2015-08-07 2015-11-25 绍兴普施康生物科技有限公司 一种离心式核酸提取纯化装置及其制作方法

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EP0504432A1 (fr) * 1990-10-09 1992-09-23 Idemitsu Petrochemical Co. Ltd. Procede d'analyse immunologique quantitative
WO1997021090A1 (fr) * 1995-12-05 1997-06-12 Gamera Bioscience Dispositifs et procedes d'utilisation de l'acceleration centripete pour commander le deplacement de liquides dans le traitement de laboratoire automatise
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WO2000005582A2 (fr) * 1998-07-21 2000-02-03 Burstein Laboratories, Inc. Dispositifs de dosage utilisant des disques optiques et methodes correspondantes
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WO1999035499A1 (fr) * 1997-12-30 1999-07-15 Remacle Jose Procede mettant en oeuvre une molecule de capture fixee sur la surface d'un disque
WO2000005582A2 (fr) * 1998-07-21 2000-02-03 Burstein Laboratories, Inc. Dispositifs de dosage utilisant des disques optiques et methodes correspondantes
WO2000026677A1 (fr) * 1998-10-30 2000-05-11 Burstein Laboratories, Inc. Disques optiques lisibles portant un materiau analyte pouvant etre lu simultanement
WO2001015154A1 (fr) * 1999-08-23 2001-03-01 Burstein Technologies, Inc. Procedes et appareil permettant de former physiquement des structures non operationnelles sur un disque optique
WO2002073605A2 (fr) * 2001-03-14 2002-09-19 Burstein Technologies, Inc. Essai biologique a deux perles utilisant des espaceurs clivables et/ou la ligature pour ameliorer la specificite et la sensibilite, procedes et dispositifs associes

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WO2002042498A2 (fr) 2002-05-30

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