WO2002026964A1 - Procede de construction d'une banque d'adn mutants et utilisation de celle-ci - Google Patents

Procede de construction d'une banque d'adn mutants et utilisation de celle-ci Download PDF

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Publication number
WO2002026964A1
WO2002026964A1 PCT/JP2001/008387 JP0108387W WO0226964A1 WO 2002026964 A1 WO2002026964 A1 WO 2002026964A1 JP 0108387 W JP0108387 W JP 0108387W WO 0226964 A1 WO0226964 A1 WO 0226964A1
Authority
WO
WIPO (PCT)
Prior art keywords
ligated
unit domain
dna
dna library
constructing
Prior art date
Application number
PCT/JP2001/008387
Other languages
English (en)
Japanese (ja)
Inventor
Toru Tsuji
Hiroshi Yanagawa
Original Assignee
Mitsubishi Chemical Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Chemical Corporation filed Critical Mitsubishi Chemical Corporation
Priority to AU2001292254A priority Critical patent/AU2001292254A1/en
Priority to JP2002530727A priority patent/JPWO2002026964A1/ja
Publication of WO2002026964A1 publication Critical patent/WO2002026964A1/fr
Priority to US10/637,009 priority patent/US20040091920A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de construction d'une banque d'ADN consistant: (a) à ligaturer des ADN à domaine unitaire en combinaisons arbitraires; (b) à mélanger les ADN ainsi obtenus de sorte que la séquence terminale de chacun de ces ADN utilisés se chevauche avec la séquence terminale d'au moins un autre type d'ADN à domaine unitaire; puis, (c) à réaliser une amplification en chaîne de la polymérase en utilisant le mélange d'ADN à domaine unitaire ligaturés comme modèle, de manière à obtenir une banque d'ADN comprenant au moins deux clones. La présente invention concerne également un procédé permettant de construire une masse de gènes, consistant à ligaturer ensemble dans des ordres différents et à des longueurs variables une pluralité de fragments d'ADN codant pour des séquences d'acides aminés arbitraires.
PCT/JP2001/008387 2000-09-27 2001-09-26 Procede de construction d'une banque d'adn mutants et utilisation de celle-ci WO2002026964A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001292254A AU2001292254A1 (en) 2000-09-27 2001-09-26 Method of constructing mutant dna library and utilization thereof
JP2002530727A JPWO2002026964A1 (ja) 2000-09-27 2001-09-26 変異ｄｎａライブラリーの構築方法およびその利用
US10/637,009 US20040091920A1 (en) 2000-09-27 2003-08-08 Method of constructing a mutant DNA library and use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2000293692 2000-09-27
JP2000-293692 2000-09-27
JP2001-29138 2001-02-06
JP2001029138 2001-02-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US39633403A Continuation 2000-09-27 2003-03-26

Publications (1)

Publication Number Publication Date
WO2002026964A1 true WO2002026964A1 (fr) 2002-04-04

Family

ID=26600800

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/008387 WO2002026964A1 (fr) 2000-09-27 2001-09-26 Procede de construction d'une banque d'adn mutants et utilisation de celle-ci

Country Status (3)

Country Link
JP (1) JPWO2002026964A1 (fr)
AU (1) AU2001292254A1 (fr)
WO (1) WO2002026964A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022625A1 (fr) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Mutagenese d'adn par fragmentation aleatoire et reassemblage
JPH1014578A (ja) * 1996-07-01 1998-01-20 Mitsubishi Chem Corp 入れ換え変異体遺伝子ｄｎａの構築

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022625A1 (fr) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Mutagenese d'adn par fragmentation aleatoire et reassemblage
JPH1014578A (ja) * 1996-07-01 1998-01-20 Mitsubishi Chem Corp 入れ換え変異体遺伝子ｄｎａの構築

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LUTZ RIECHMANN ET AL.: "Novel-folded protein domains generated by combinatorial shuffling of polypeptide segments", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 18, August 2000 (2000-08-01), pages 10068 - 100073, XP002178866 *
MARC OSTERMEIER ET AL.: "A combinatorial approach to hybrid enzymes independent of DNA homology", NATURE BIOTECHNOLOGY, vol. 17, no. 12, 1999, pages 1205 - 1209, XP001013770 *
TORU TSUJI: "Tanpaku -shitsu no shinka bunshi kougaku", KAGAKU KOGYO (CHEMICAL INDUSTRY), vol. 51, no. 5, May 2000 (2000-05-01), pages 329 - 336, XP002907457 *

Also Published As

Publication number Publication date
AU2001292254A1 (en) 2002-04-08
JPWO2002026964A1 (ja) 2004-02-05

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