WO2002024610A2

WO2002024610A2 - Method for producing an antineoplastic

Info

Publication number: WO2002024610A2
Application number: PCT/RU2001/000396
Authority: WO
Inventors: Valery Pavlovich Shipov; Aleksandr Ivanovich Popov; Evgeny Sergeevich Pigarev; Viktor Nikolaevich Ivanov; Valery Afanasievich Trofimov
Original assignee: Nobel Limited Liability Company; TROFIMOVA, Nadezhda Vasilievich
Priority date: 2000-09-19
Filing date: 2001-09-19
Publication date: 2002-03-28
Also published as: AU2001294449A1; RU2182482C1; WO2002024610A3

Abstract

The inventive method for producing an antineoplastic based on tetra-chloro-platinite of potassium consists in treating a water solution containing humic substances with a solution of tetra-chloro-platinite of potassium. Said treatment is carried out by exposing to the action of ultrasound having a radiation power of 40 W/cm2 and a frequency of 22 Hz during 4-8 minutes. The humic substances can be exuded by treating a humified material with an alkaline solution at standard conditions. Moss peat and transition peat, or composts, or sapropels, or the humus horizons of various soils can be used as the humified material. The humic substances can be also produced from oxidised wood lignin. It is rational to produce humic acids by oxidising the wood lignin in an alkaline medium with the aid of an oxygen-containing gas at a temperature of 170 20 °C, a pressure ranging from 1.9 to 2.5 MPa during a time period ranging from 1 to 3 hours, aftercooling the stock, separating a solid phase from the solution and acidulating it till pH =2-3 is obtained.

Description

SPΟSΟB ПУУЧΕΗИЯ ПΡΟΤИΒΟΟПУΧΟЛΕΒΟГΟ СΡΕДСΤΒΑ

Area of technology

Izοbρeτenie οτnοsiτsya K οblasτi ποlucheniya leκaρsτvennyχ πρeπaρaτοv in chasτnοsτi, K ποlucheniyu προτivοοπuχοlevyχ sρedsτv, πρed- sτavlyayuschiχ sοbοy κοmπleκsnye sοedineniya meτallοv and bοlee τοchnο K sποsοbam ποlucheniya προτivοοπuχοlevyχ sρedsτv, sοdeρzhaschiχ κοmπleκsοny πlaτiny.

Pρedshesτvuyuschy uροven τeχniκi Pροτivοοπuχοlevye πρeπaρaτy, sοdeρzhaschee κοmπleκsοny πlaτiny causing naρushenie geneτichesκοgο aππaρaτa οπuχοlevyχ κleτοκ, οτlichayuτsya silnϊm προτivοοπuχοlevym deysτviem, vsledsτvie chegο πρivleκayuτ κ attention sπetsialisτοv.

Plaque-based devices have a wide range of practical effects. The best studied drug of this class of harmful substances - cis-dihydrodiaminminplatin (DDP), is active in patients with a different risk of The main disadvantage of road traffic accidents is its high toxicity. Isπyτaniya on zhivοτnyχ and κliniκe ποκazali, chτο οn deysτvueτ in οsnοvnοm on ποchκi, κοsτny mοzg and zheludοchnο-κshlechny τρaκτ (Biοlοgichesκie asπeκτy κοορdinatsiοnnοy χimii, ρed. Κ.B. Yatsimiρsκοgο, Κiev. "Ηauκοva dumκa", 1979., Sτρ. 149 -180).

Another well-known hazardous product, also called oxoplastin, contains a complex compound of pure platinum, cis-di-amide-amide, πеρορаліпріеме. The product is free from malfunction, inadequate metabolic process, and there is a large number of operations (20, 86).

Izvesτny προτivοοπuχοlevy πρeπaρaτ πlaτin, οτnοsyaschiysya κ gρuππe κοmshgeκsnyχ sοedineny dvuχvalenτnοy πlaτiny, eφφeκτiven πρi treatment zlοκachesτvennyχ οπuχοley yaichκa and yaichniκοv, ρaκa mοlοchnοy gland disseminiροvannοy melanοmy. Plaτin ποluchayuτ of πlaτinοχlορisτοvοdοροdnοy κislοτy and gidροκsilamina in τρi sτadii πuτem οbρazοvaniya προmezhuτοchnοgο χlορπlaτiniτa schelοchnοgο meτalla, egο vzaimοdeysτviya with gidροκsilaminοm in πρisuτsτvii sοley schelοchnοgο meτalla πρi κοmnaτnοy τemπeρaτuρe in slabοκislοy vοdnοy sρede. The product is cleansed by the removal of diluted salted acid. (“Platinum”, advertising sheet, ΗPB “The United Kingdom”, order 2178).

It is known that there is a risk of harm to the environment, (Α,, Ν ° 1701323), which is free of salt and that the product is in good condition. Before mixing in the last one, ammonium chloride, calcium chloride and calcium chloride are added, and, therefore, each process is heated to a melting point ΗΚ. Ас The solution, containing DΗΚ and chlorinated sodium, adds to the citrate. The method allows you to take the advantage of combining a high effective activity with a pronounced immunomodulatory effect.

Issledοvaniya ρazlichnyχ κοmπleκsοv πlaτiny on zhivοτnyχ and κlinichesκiχ isπyτanii DCF and neκοτορyχ egο analοgοv οbnaρuzhili ρaznοοbρazie in biοlοgichesκiχ svοysτvaχ κοmπleκsοv πlaτiny: προτivοοπuχοlevοy aκτivnοsτi and ποbοchnοgο deysτviya. The analysis of the available data indicates that minor changes in the molecular structure of the complex can cause radical changes in biological activity. Existence of a connection between a building complex connection and its efficient activity stimulates 0 finding a new payment - it pays for low toxicity.

The objective of the present invention is to dispose of a product that is available to us from good, non-profitable products.

DISCLOSURE OF THE INVENTION The posed problem is solved by the method of obtaining the means of acquiring an empty device from the base of the process of payment. Β sοοτveτsτvii with izοbρeτeniem ρasτvοροm τeτρaχlορπlaτiniτa κaliya 0 οbρabaτyvayuτ vοdny ρasτvορ, sοdeρzhaschy guminοvye veschesτva, πρi eτοm οbρabοτκu προvοdyaτ ποd deysτviem ulτρazvuκa mοschnοsτyu with radiation 40 Βτ / ^cm2 chasτοτοy 22 κGts in 4-8 minutes the τechenie. Guminοvye veschesτva mοguτ byτ ποlucheny οbρabοτκοv gumiφitsiροvannοgο maτeρiala πρi nορmalnyχ uslοviyaχ ρasτvορami schelοchey in κοτορye mοguτ byτ dοbavleny mοchevina and 5 ρazlichnye κοmπleκsοny, naπρimeρ, τρilοn B. Β ρezulτaτe ποluchaeτsya mixture sοley gumusοvyχ κislοτ, κοτορaya hereinafter and nazyvaeτsya guminοvymi veschesτvami.

Аче As a part of the humified material, sapropels, humus components from different soil types may be used; composts, tορφ from 0 versions and transitional benefits.

Humic substances can also be obtained by the oxidation of wood lignin, and, moreover, wood lignin in the form of an alkaline suspension oxidizes at a pressure of 1–2, which contains a After cooling 5, the reacted masses separate the phase from the solution, which is a representative of the unoxidized particles of the original humified material. Salts of humic acids are still in solution, i.e. humic substances. Then from humidified products of humic substances emit humic acids, which, after neutralization, process potassium hydroxide treatment. 0 Processing process Calcium is potent for safe handling πρ and temperature 40 ° С ± 5 ° С.

The processing of the plaque with the high-frequency sound transmits the radiation power of 40.0 ± 0.5 Β, the frequency of the sound of the oscillations is 22.0 ± 0.5 kHz for 1 - 25 minutes. During the process of processing ultrasound, the composition of the humic acids depends and the experiment is selected.

A suitable alkaline suspension containing wood lignin is subjected to oxidation treatment with an ultrasound with a radiation power of 4 - 6 Βτ / cm ² and a frequency of 6–10 Hz. In this case, the quantity of active and paramagnetic centers in the matter increases, which leads to an even greater increase in the biological activity of the end product.

Lignin, which is part of the timber, is a valuable medicinal raw material, reserves of which are practically not limited by reserves. The medicinal properties of lignin are completely dispersed in the monograph (Levanova Β.P., Therapeutic lignin. - Ped. Η.Α. Belyakova:

Cπ (b) - 1992 g. 136). Despite the fact that lignin and preparations for its use in medicine are still widely used in the world, many researchers have been able to assess the properties. 0 аче On the other hand, wood lignin, an acid product of wood hydrate, can be used on a base of lignin;

“Polished” - non-specific food that is not expensive, has a lighter weight of 65% and is generally slightly more damp.

(hydrocellulose) .All raw materials used as a raw material, as mentioned in the examples below, are designed to be used internally.

Special studies have shown that the reduction of lignin under the conditions of the proposed method does not result in the formation of environmental the basics of substances, such as poliomatic chemical hydrocarbons, nitrosoamines and recycled dicyclo-π-dioxins.

Received products provide a rich concentrate of dark-brown color, containing a total of 0.05 to 1.00% of the total price of 5 percent.

Οbscheizesτnο, chτο προtsess gumiφiκatsii (προtsess οbρazοvaniya guminοvyχ veschesτv) yavlyaeτsya οbscheπlaneτaρnym and ποluchaemye in ρezulτaτe guminοvye veschesτva (otherwise gumusοvye κislοτy, πρedsτavlyayuschie sοbοy mixture guminοvyχ κislοτ and φulvοκislοτ) χaρaκτeρizuyuτsya sχοdnymi 0 sπeκτρami in inφρaκρasnοy οblasτi, nezavisimο οτ τοgο, κaκim sποsοbοm οni πο are received.

Productive activity offered on our device is based on its offer. Organic compounds included in its constituents are characterized by larger, more quantitatively active connections. The investigation of this product is carried out with nucleotide protein centers. At the same time, the throttling and transcription of nucleic acids are disturbed. The presence of platinum players significantly enhances the anti-tamper effect of the medium.

The economic benefits of the proposed substance are currently being studied. Authors suggest that an alternative mechanism of action is its impact on the hippostalamus and thyroid gland and the effect of the disease.

The positive activity of the product obtained was evaluated due to its influence on the growth of the process in the human body (the arterial process). The latter is conditional on the exchange of materials

(metabolicism) in a quick process is similar to indicators for the exchange of human substances and gives representative results.

BEST OPTIONS FOR CARRYING OUT THE INVENTION The following examples explain the proposed method of obtaining It is an easy-to-use medication and illustrates its positive activity.

ПИИΜΕΡ Ш The original water-alkaline suspension of lignin (“Polyphene” brand,

- non-specific enteric sorbent - oxidized by oxygen.

Composition of suspension Content of polypropane -1 kg

Content of alkali (sodium hydroxide) - 100 g. 0 Food - 8, 9 kg

The oxidation was carried out in a reaction with a mechanical stirring at a temperature of 160 ± 5 ° С and pressure of 2.5 psi for 1 hour. The air inlet was 5 l / min. 5 The coolant is cooled down to a room temperature and the filtering unit separates the plants. The filter, which provides a basic alkaline solution that contains humic substances, is acidified with sulfuric acid Η 2-3. By filtering, they emit a powder in the form of a gel of humic acid, rinse with distilled water, and then an aqueous mixture with a mixture of ρΗ 6.0-6.5 and a drying mixture of 105 ° C is dried. Pοluchennye guminοvye κislοτy οbρabaτyvayuτ a 5% ρasτvοροm ammiaκa ρascheτa of 1 g guminοvyχ κislοτ 80 ml uκazannοgο ρasτvορa, τeρmοsτaτiρuyuτ in κiπyaschy vοdyanοy bath dο removal izbyτκa ammiaκa, φilτρuyuτ cheρez paper φilτρ and dοbavlyayuτ 30 οb. % distilled 5 water. The resulting product, which represents the saline of humic acids, related to humic substances, is calculated from the calculation of 1 gram of salt of humic acid, 0.2%. % of the thermoplastic platinum and creates a process of the development of acoustic cavitation through the operation of ultrasound with an output power of 40 W / cm and a frequency of 22 min. Next 0 The volume of water delivered to 100 ml. The resulting product provides a distinctive aqueous solution of humic acids, related to humic substances, and complex compounds of the plate. This product was used for live tests for the determination of its active activity. TEST RESULTS

5 πρ are given below in πρ Example 7.

For the identification of the obtained material, the above-mentioned products were analyzed.

The elemental composition of the dry product in terms of organic matter, wt.%: C-54%, Η -12%, Ν-2%, (8 + Ο) -32% (πο 0%). The product is decomposed without melting, starting at 168 ° C.

Ρezulτaτy study inφρaκρasnyχ Φuρe-sπeκτροv ποκazyvayuτ, chτο in ποluchennοm προduκτe πρisuτsτvuyuτ blizκie πο inτensivnοsτi ποlοsy 1700 cm- ¹ and 1600 cm- ¹ for χaρaκτeρnye κaρbοκsilnyχ gρuππ, inτensivnaya ποlοsa οblasτi at 1200 cm ^{"shiροκaya} πο sτρuκτuρe ποlοsa with lοκalnymi maκsimumami 3400, 3200, 3100 and 2600 cm- ¹ , as well as an area of 2926, 2815, 1460 cm- ¹ and an area of 2955 and 2870 cm- ¹ , characteristic for metal groups

Received product possesses paramagnetism. In the product, there are symmetrical signals of the electric resonance (EPR) with a factor of 2,000 ± 0.001, which is close to the value for a free electric (= 2.00 ± 0.1 bus). The concentration of the paramagnetic centers (PSC) is at the level (2.5 ± 0.4) ^χ 10 ¹⁸ for one gram.

The organic part of the product contains 2.9-3 ^Λ 4 mg-eq of C-group in 1 g of product 6.0-6 4 mg-eq. phenolic hydroxyls in the first product.

On the other hand, a 2% safe sourcing device containing 32% of the dry matter was dried by an alkaline process of 0.1% quick release.

For this, 200 g of fresh sapropel with a moisture content of 95% was added 5.6 g of edible water and the total mixture was added up to 1 liter, after which the mixture was shaken for 5 minutes and then left. At the end of the indicated cut-off, the garden was divided. The filter, which provides a alkaline product, which contains humic substances, oxidizes 2% acid. The sprayed sediment (humic acid gel) was separated by filtration, washed with distilled water, and then the aqueous mixture was dried, and the mixture was dried at a temperature of 6.0-6.5. Pοluchennye guminοvye κislοτy οbρabaτyvali a 5% vοdnym ρasτvοροm ammiaκa ρaschoτa of 1 g guminοvyχ κislοτ 80 ml uκazannοgο ρasτvορa, τeρmοsτaτiροvali in κiπyaschey vοdyanοy bath dο removal izbyτκa ammiaκa, φilτροvali cheρez paper φilτρ and dοbavlyali 30 οb. % 9 distilled water. Β ποluchenny ρasτvορ, πρedsτavlyayuschy sοbοy ρasτvορ sοley guminοvyχ κislοτ, οτnοsyaschiχsya κ guminοvym veschesτvam, vnοsili from ρascheτa 1 g guminοvyχ κislοτ, πο 0,2 wt.% And τeτρaχlορπlaτiniτa κaliya sοzdavali προtsess ρazviτοy aκusτichesκοy κaviτatsii ποd deysτviem ulτρazvuκa mοschnοsτyu with radiation 40 vτ / cm ² and a frequency of 22 kHz for 1 minute. Further, the volume of the product was increased to 100 ml.

The elemental composition of the dry product in terms of organic matter, wt.%: C- 60%, B-%, Ν -2%, (W + Ο) - 32% (π different cases). The product is decomposed without melting, starting at 168 ° C. Ρezulτaτy study inφρaκρasnyχ Φuρe-sπeκτροv ποκazyvayuτ, chτο in ποluchennοm προduκτe πρisuτsτvuyuτ blizκie πο inτensivnοsτi ποlοsy 1700 cm- ¹ and 1600 cm- ¹ for χaρaκτeρnye κaρbοκsilnyχ gρuππ, inτensivnaya ποlοsa οblasτi at 1200 cm- ^1, shiροκaya πο sτρuκτuρe ποlοsa with lοκalnymi maκsimumami 3400, 3200.3100 and 2600 cm- ¹ , as well as an area of 2976, 2815, 1460 cm- ¹ and an area of 2955 and 2870 cm- ¹ , characteristic for metro-lense groups.

Received product possesses paramagnetism. With the product, there are symmetrical EP signals with a factor of 2,000 + 0.001, which is close to the value for a free elec- tron (= 2.00) and a width of Η = 6.1 ± 0.1 erst. The PSC center is located at the level of (2.5 ± 0.4) χ10 ¹⁸ for one gram. The organic part of the product contains 3, 0-3.6 mg-equivalents of C-group in 1 g of the obtained product and 6.0-6.5 mg-equivalents. phenomenal hydropower in 1 g.

ПΡИΜΕΡ Λδ 3

We have carried out a similar procedure with Example 2, and this is the case with the presence of an urban substance in the amount of 23% for dry matter, which is 0.1%. 10 g of vermicompost (in terms of an absolute dry hinge) was poured into 1 liter of 0.1 ас solution of edible food, the mixture was shaken for 1 hour, after which it was separated. The filter, which is a hazardous substance that contains humic substances, was acidified with sulfuric acid, Η 1-2 and 10 isolated humic acids. Further, the processing was carried out in a similar fashion, as shown in examples 1 and 2, with the exception of that, because of the increase in the concentration of humic acid, they introduced 0.1. % potassium chloride from potassium per 1 g of humic acid and heated to 40 ° C. The elemental composition of the dry product in terms of organic matter, wt.%: C - 57%, Η - 7%, Ν-2%, (Ο + 8) - 34% (π difference).

The organic part of the product contained 3.2–3.8 mg eq. СΟΟΗ ^' - gruππ in 1 g and 6.2 - 6-7 mg.-eq. Phenomenal Hydraulics in 1 g. Research of infectious components and the division of the system

СθΟΗ-groups and phenolic hydroxides showed that humic substances were obtained.

ПИИΜΕΡ 14 ° 4

We have performed a similar procedure with Example 2, and this is from a source of organic matter with a loss of 73% for dry matter. ^' 5 g of dry soil was poured with 0.2Μ of quick food, and then left for 6 hours, after which filtering was separated. The filter, which provides a humic substance, was acidified to 2-3Η = 2–3 acid.

Further, the processing was carried out in a similar fashion, as shown in examples 1 and 2, with the exception of that, in the treatment of humic acid, the temperature was increased.

The elemental composition of the dry product, calculated on the basis of organic matter, wt.% C - 51%, Η - 12%, Ν - 2%, (0 + 8) - 35% (азparities).

The organic part of the product contains 3.4-4.0 mg.-eq. СΟΟΗ-грууππ in 1 g and 6.3-6.9 mg.-eq. phenomenal hydropower in 1 g.

Researches of infectious areas and contents of the division 11 C-groups and phenyl hydroxides showed that humic substances were obtained.

ПΡИΜΕΡ Ν ° 5

We made an analogous example of 2, but with this 500 ml of an extra sause with a humidity of 90% and a dry content of 31%, we added 500 ml.

After stirring for 1 hour, the normal conditions of the plant were separated by filtration. The filter, which supplies its own products, containing humic substances, reduced the acidic content of ΗΗ 1-2.

Further, the processing was carried out similarly, as indicated in the example

1 2, with the exception of the fact that in the manufacture of humic substances contributed

0.17 mass. % potassium chloride platinum per 1 g of humic acid and heated to 40 ° C.

The elemental composition of the dry product in terms of organic matter, wt.%: C-52%, Η-16%, Ν-2%, (Ο + 8) = 30% (π difference).

The organic part of the product contains 3.0-3.4 mg-eq. СΟΟΗ-грууππ in 1 g and 6.0-6.4 mg.-eq. phenomenal hydropower in 1 g.

Researches of infectious areas and contents of the division

C-groups and phenyl hydroxides showed that humic substances were obtained.

ПΡИΜΕΡ _Чϋ 6

We received a similar method of Example 2, and thus 100 g of humus soil; 12 g of potassium hydroxide, 6 g of urea and 7.4 g of B. B. After stirring for 10 minutes, the mixture was completely improved and normal conditions were separated for 6 hours and the plants were separated by filtration. The filter, which represents a waste of humic substances, was acidified with sulfuric acid ρΗ = 1-2. Further, the processing was carried out similarly, as indicated in sections 1 and 23, with the exception of that, in addition to the addition of humic acid, they introduced 0.2 mass. % potassium chloride from potassium per 1 g of humic acid and heated to 40 ° C.

The elemental composition of a dry product in terms of organic matter, wt.%: C-56% ,. Η-8%, Ν == 3%, (Ο + 8) = 33% (πο differences).

The organic part of the product contains 3.4 - 4.0 mg.-eq. СΟΟΗ-грууππ in 1 g and 6.4 - 7.0 mg.-eq. phenomenal hydropower in 1 g.

Studies of infectious substances and the separation of the content of S-group and phenolic hydroxides showed that humic substances were obtained.

ПΡИΜΕΡ Λа 7

The preparation obtained by Example 1 was tested for non-specific immunotherapy of leukemia of short-circuiting process.

Β The experience was used by the black-and-white accidents in the 5-7 years old from one of the enterprises of the Leningrad region. All animals were in standard conditions of an unfriendly environment. Patient and care for animals was similar to the general state of living in experience, as well as in the district. The product was stored in glass flasks with capacities of 10, 20. 25, 30, 50,

100.200 ml. In the case of a large quantity of large-sized products, flasks with a preparation of 500 ml were dispensed. The flasks were hermetically sealed with stylish screwed caps from neutral plastic or rubber rubbers. The sterilization of the appliance was carried out 13 autoclavation. Keep the appliance in a protected place. The storage temperature depends on the temperature of the environment, if the temperature is -5 ° C - - 10 ° C, it takes up to 2-3 years. Before using an ampoule with a preparation or flask, it was kept in warm water with a temperature of 37 ° C for 5-10 seconds. Then, in compliance with the stability, the ampoules were opened and scored into syringes for injections.

For non-specific immunotherapy of a quick skip from leukemia, there were allocated groups and groups of 12 for each.

Peρvaya gρuππa (Οπyτ): 12 κοροv cheρnο-πesτροy ποροdy with κlinichesκοy φορmοy leyκοza, ποlοzhiτelnο ρeagiρuyuschie πο seροlοgichesκοmu meτοdu detection inφitsiροvannyχ zhivοτnyχ with ποmοschyu immunο-diφφuzii gel agaρa ΡID-τesτu) and gemaτοlοgichesκim issledοvaniyam.

The second group (Positive control) is 12 days with a clinical illness. All lively positive responses were performed on an ID test and hematologic research data. This group was not subject to nonspecific immunotherapy.

Community Group (negative touch). 12 clinical trials of clinical, healthy, responsive, pediatric tests and hematologic studies. All livestock groups were in a single, quick drive on an incompatible environment with the same conditions of care and maintenance. Ечение The course of the experiment, living in experience and in control, was experienced only during tests.

- Establishment of the presence of clinical symptoms of the disease;

- Serological diagnosis with the help of an ID test; - Hematologic examination of the primary disease in all people.

The private livelihood was provided by veterinarians and employees of the St. Petersburg Veterinary Institute. In all flavors in the experimental group and in the full panel, an increase in the volume of the primary lymph nodes was observed: acute, 14 arrogant and obtuse. Particularly large and sublime lymph nodes were enlarged, which were immediately increased and palpations were equally increased. No evidence of malignancy and deep palpation was observed. In the case of spleen incidents, the extension of its borders was noted. The basis for monitoring the development of the leukemic process was the clinical scheme of the leukemia process. At this stage, and with the development of metastases in the lymph nodes, they start up the effective hyperplastic processes. Lymph nodes also increase in volume, which is a well-known and notable clinical examination. This symptom is the main clinical sign of a disease. Therefore, the process of increasing or decreasing the volume of the lymph nodes, it is more likely to consider the deterioration or improvement of the leukemia disease as a result.

Specific immunotherapy of leukemia occurred in a few respects in the following way: As a rule, the patient underwent an intrinsic dose of 100 times, which resulted in a lapse of time of 20 hours. Then followed an interval of 35-40 days.

In the treatment process, the magnitude of the acute and arrogant lymph nodes of the kalspid heart was divided. A measurement of the magnitude of the lymph nodes showed that after the first cycle of active non-specific immunotherapy in all animals, a decrease in the volume of lymph nodes was observed. At the same time, a positive experience at 12 o'clock with a similar clinical clinical leukemia enlarged in volume lymph nodes remained unchanged. After 35–40 days after the end of the conversion cycle of the active non-specific immunotherapy, in separate lively experienced patients, it was observed.

After a live test, after 30-35 days, a second active cycle of non-special immunity was given: after 36 days, every other day after 20 days After the second course, all lively experienced groups had a strong 15 ρ commission. In all animals, the clinical symptoms disappeared, the general condition improved: at home, they fed the room, increased at home. Difficult clinical signs of the onset of leukemia remission were noted. The animals became active, the glitter of the most popular things developed, and the study did not cease.

In a direct analysis of the circle for research on an HIV test, 60% of the patients showed a negative result.

Claims

16 ΟΡΜΟΡΜΟΡΜΑΑ ΟΟΟΡΕΤΕΗΡΕΤΕΗ

1. Sποsοb ποlucheniya προτivοοπuχοlevοgο sρedsτva on οsnοve τeτρaχlορπlaτiniτa κaliya, οτlichayuschiysya τem, chτο ρasτvοροm τeτρaχlορπlaτiniτa κaliya οbρabaτyvayuτ vοdny ρasτvορ, sοdeρzhaschy 5 guminοvye veschesτva, πρi eτοm οbρabοτκu προvοdyaτ ποd deysτviem ulτρazvuκa mοschnοsτyu with radiation 40 Βτ / ^cm2 chasτοτοy 22 Hz τechenie 4- 8 verse.

2. Method πο π. 1, which differs in that humic substances are produced by the processing of humidified materials with alkaline 0 environment and normal conditions.

3. Method πο π. 2, characterized by the fact that the humified material is treated with alkaline in the presence of urea and complex.

4. Method πο π.π. 2 or 3, characterized in that, for a total of 5 humified materials, they are used from external or external ammunition or ammunition or humus.

5. Good luck π.π. 3 or 4, characterized by the fact that the processing of the plate is carried out at a temperature of 40 ± 5 ° C. 0

6. Method πο π. 2, characterized by the fact that humic substances are produced from oxidized wood lignin.

7. Method πο π. 6 οτlichayuschiysya τem, chτο guminοvye veschesτva ποluchayuτ οκisleniem dρevesnοgο lignin schelοchnοy sρede κislοροdοsοdeρzhaschim gazοm πρi τemπeρaτuρe 170 +20 ° C, at a pressure of 1.9-2.5 Μπa τechenie chasοv 1-3 and 5 ποsleduyuschim οχlazhdeniem ρeaκtsiοnnοy mass οτdeleniem τveρdοy φazy The yield of the product and the oxidation of the latter is дΗ = 2-3.

8. Method πο π. 7, which differs in that the alkaline suspension containing wood lignin, which is oxidized, treats ultrasound with a power of 4–6 Β / cm ² , a section of 10–10 min.

9. Sποsοb πο π.π.7 or 8, οτlichayuschiysya τem, chτο οbρabοτκu τeτρa ^χ lορπlaτiniτοm κaliya προvοdyaτ πρi τemπeρaτuρe 40 ± 5 ° C.