WO2002016573A3 - Mammifere non humain, genetiquement modifie, contenant un gene regulateur supplementaire inductible - Google Patents

Mammifere non humain, genetiquement modifie, contenant un gene regulateur supplementaire inductible Download PDF

Info

Publication number
WO2002016573A3
WO2002016573A3 PCT/DE2001/003158 DE0103158W WO0216573A3 WO 2002016573 A3 WO2002016573 A3 WO 2002016573A3 DE 0103158 W DE0103158 W DE 0103158W WO 0216573 A3 WO0216573 A3 WO 0216573A3
Authority
WO
WIPO (PCT)
Prior art keywords
regulator gene
human mammal
gene
genetically modified
cells
Prior art date
Application number
PCT/DE2001/003158
Other languages
German (de)
English (en)
Other versions
WO2002016573A2 (fr
Inventor
Claus Scheidereit
Ruth Schmidt-Ullrich
Original Assignee
Max Delbrueck Centrum
Claus Scheidereit
Ruth Schmidt-Ullrich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Delbrueck Centrum, Claus Scheidereit, Ruth Schmidt-Ullrich filed Critical Max Delbrueck Centrum
Publication of WO2002016573A2 publication Critical patent/WO2002016573A2/fr
Publication of WO2002016573A3 publication Critical patent/WO2002016573A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un mammifère non humain transgénique, de préférence une souris, dont les cellules corporelles et sexuelles contiennent un gène régulateur inductible pouvant être exprimé dans toutes les cellules. L'activité biologique du gène régulateur est obtenue après croisement avec un animal portant un gène de la recombinase (Cre). Ce gène régulateur contient, pour assurer son expression en fonction de Cre, un codon d'arrêt, flanqué d'éléments loxP, en amont du site de départ de la traduction. Ce gène régulateur a été transmis à l'animal d'origine par transfection et recombinaison homologue de cellules souches embryonnaires puis par micro-injection de blastocytes.
PCT/DE2001/003158 2000-08-25 2001-08-24 Mammifere non humain, genetiquement modifie, contenant un gene regulateur supplementaire inductible WO2002016573A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000141842 DE10041842A1 (de) 2000-08-25 2000-08-25 Genetisch verändertes, nicht-menschliches Säugetier, das ein zusätzliches induzierbares Regulator-Gen enthält
DE10041842.2 2000-08-25

Publications (2)

Publication Number Publication Date
WO2002016573A2 WO2002016573A2 (fr) 2002-02-28
WO2002016573A3 true WO2002016573A3 (fr) 2002-06-20

Family

ID=7653797

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003158 WO2002016573A2 (fr) 2000-08-25 2001-08-24 Mammifere non humain, genetiquement modifie, contenant un gene regulateur supplementaire inductible

Country Status (2)

Country Link
DE (1) DE10041842A1 (fr)
WO (1) WO2002016573A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004038008A2 (fr) * 2002-10-25 2004-05-06 University Of Massachusetts Modulation de la proliferation cellulaire
CN104335970B (zh) * 2013-08-05 2016-08-24 应哲康 一种构建人银屑病小鼠模型的方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779361A2 (fr) * 1995-12-15 1997-06-18 F. Hoffmann-La Roche Ag Forme tronquée de la protéine inhibitrice kappa B, production récombinante et utilisation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779361A2 (fr) * 1995-12-15 1997-06-18 F. Hoffmann-La Roche Ag Forme tronquée de la protéine inhibitrice kappa B, production récombinante et utilisation

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BOOTHBY MARK R ET AL: "Perturbation of the T lymphocyte lineage in transgenic mice expressing a constitutive repressor of nuclear factor (NF)-kappa-B.", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 185, no. 11, 1997, pages 1897 - 1907, XP002051805, ISSN: 0022-1007 *
EMMERICH FLORIAN ET AL: "Overexpression of I kappa B alpha without inhibition of NF-kappaB activity and mutations in the I kappa B alpha gene in Reed-Sternberg cells.", BLOOD, vol. 94, no. 9, 1 November 1999 (1999-11-01), pages 3129 - 3134, XP002188140, ISSN: 0006-4971 *
HATADA EUNICE N ET AL: "NF-kappaB and the innate immune response.", CURRENT OPINION IN IMMUNOLOGY., vol. 12, no. 1, February 2000 (2000-02-01), pages 52 - 58, XP002188143, ISSN: 0952-7915 *
HUELSKEN JOERG ET AL: "Requirement for beta-catenin in anterior-posterior axis formation in mice.", JOURNAL OF CELL BIOLOGY., vol. 148, no. 3, 7 February 2000 (2000-02-07), pages 567 - 578, XP002188141, ISSN: 0021-9525 *
KRAPPMANN DANIEL ET AL: "Different mechanisms control signal-induced degradation and basal turnover of the NF-kappa-B inhibitor I-kappa-B-alpha in vivo.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 15, no. 23, 1996, pages 6716 - 6726, XP002188139, ISSN: 0261-4189 *
LUO JUN-LI ET AL: "Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: A new biomedical research tool.", ONCOGENE, vol. 20, no. 3, 2001, pages 320 - 328, XP002188144, ISSN: 0950-9232 *
SAUER B: "INDUCIBLE GENE TARGETING IN MICE USING THE CRE/LOX SYSTEM", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 14, no. 4, April 1998 (1998-04-01), pages 381 - 392, XP001019712, ISSN: 1046-2023 *
SLAUGENHAUPT SUSAN A ET AL: "Tissue-specific expression of a splicing mutation in the IKBKAP gene causes familial dysautonomia.", AMERICAN JOURNAL OF HUMAN GENETICS, vol. 68, no. 3, March 2001 (2001-03-01), pages 598 - 605, XP002188142, ISSN: 0002-9297 *
TALITHA R. BAKKER ET AL.: "Impaired Fetal Thymocyte Development After EfficientAdenovirus-Mediated Inhibition of nF-kB Activation", THE JOURNAL OF IMMUNOLOGY, vol. 162, 1999, pages 3456 - 3462, XP002188138 *
VAZQUEZ J C ET AL: "FACTORS AFFECTING THE EFFICIENCY OF INTRODUCING PRECISE GENETIC CHANGES IN ES CELLS BY HOMOLOGOUS RECOMBINATION: TAG-AND-EXCHANGE VERSUS THE CRE-LOXP SYSTEM", TRANSGENIC RESEARCH, LONDON, GB, vol. 7, no. 3, May 1998 (1998-05-01), pages 181 - 193, XP000864574, ISSN: 0962-8819 *

Also Published As

Publication number Publication date
DE10041842A1 (de) 2002-03-07
WO2002016573A2 (fr) 2002-02-28

Similar Documents

Publication Publication Date Title
WO2003064627A3 (fr) Oiseau chimerique forme a partir de cellules souches embryonnaires
US20130212725A1 (en) Fusion proteins comprising a dna-binding domain of a tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
WO1999053017A3 (fr) Procedes et constructions vecteurs pour produire des animaux transgeniques exprimant de maniere ubiquiste un gene heterologue
RU2014153918A (ru) Способы и композиции для получения условно нокаутных аллелей
DE60327717D1 (de) Zufuhr transposonbasierter vektoren zu fortpflanzungsorganen
BR9806872A (pt) Transferência nuclear com células diferenciadas doadoras fetais e adultas
DE69835145D1 (de) Transgene Mäuse die ein menschliche Antikörper exprimieren
WO2004015123A8 (fr) Expression tissulaire specifique de proteines exogenes chez des poulets transgeniques
WO2006089294A3 (fr) Expression tissulaire specifique d'anticorps chez les poulets
Isotani et al. Generation of Hprt-disrupted rat through mouse← rat ES chimeras
Fatema et al. Gamete nuclear migration in animals and plants
Plummer et al. A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre‐dependent FLEx switch
WO2002016573A3 (fr) Mammifere non humain, genetiquement modifie, contenant un gene regulateur supplementaire inductible
WO2001036482A8 (fr) Souris transgeniques exprimant une proteine fluorescente
Hirabayashi et al. A retrospective analysis of germline competence in rat embryonic stem cell lines
WO2004007753A3 (fr) Mammiferes transgeniques non humains exprimant un acide nucleique reporteur dans des conditions de regulation d'elements de reponse d'androgenes
US20240263194A1 (en) Animal preparation method
EP2530163A1 (fr) Lignée cellulaire à double marqueur
Vermot et al. Conditional (loxP‐flanked) allele for the gene encoding the retinoic acid‐synthesizing enzyme retinaldehyde dehydrogenase 2 (RALDH2)
Jia et al. R/L, a double reporter mouse line that expresses luciferase gene upon Cre-mediated excision, followed by inactivation of mRFP expression
AU2004274707A1 (en) Excretable reporter systems
JPWO2005123918A1 (ja) 外来遺伝子の誘導発現の制御が可能な発現ベクター
NO20021538D0 (no) Fremgangsmåter for fremstilling av klonede og transgene pattedyr
Ma et al. 304 HUMAN AUGMENTER OF LIVER REGENERATION TRANSGENIC OVINE EMBRYO DERIVED FROM SOMATIC CELL NUCLEAR TRANSFER
Crispo et al. Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase