WO2002000169A3 - Production of vaccines using transgenic plants - Google Patents

Production of vaccines using transgenic plants Download PDF

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Publication number
WO2002000169A3
WO2002000169A3 PCT/US2001/020272 US0120272W WO0200169A3 WO 2002000169 A3 WO2002000169 A3 WO 2002000169A3 US 0120272 W US0120272 W US 0120272W WO 0200169 A3 WO0200169 A3 WO 0200169A3
Authority
WO
WIPO (PCT)
Prior art keywords
bymv
ndv
plants
transgenic
pvy
Prior art date
Application number
PCT/US2001/020272
Other languages
French (fr)
Other versions
WO2002000169A2 (en
Inventor
Rosemarie Hammond
Yan Zhao
John Hammond
Original Assignee
Us Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Agriculture filed Critical Us Agriculture
Priority to CA002414396A priority Critical patent/CA2414396A1/en
Priority to AU2001270164A priority patent/AU2001270164A1/en
Publication of WO2002000169A2 publication Critical patent/WO2002000169A2/en
Publication of WO2002000169A3 publication Critical patent/WO2002000169A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
    • C12N2760/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/34011—Potyviridae
    • C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Plants infected with potato virus Y (PVY) were inoculated with infectious PVX RNA encoding PVX coat protein (CP) and modified chimeric NDV/BYMV CP. Antigen-coated plate indirect ELISA and immunoelectron microscopy of virus purified from infected plants showed that progeny virions contained from <1% to as much as 25% chimeric CP. The method can be used to produce and purify large amounts of NDV vaccine. Transencapsidated PVY virions expressing NDV were purified and used to induce anti-NDV antibodies in mice. Further, transgenic tobacco plants expressing HIV/BYMV CP were inoculated with BYMV. Progeny virions from transgenic plants contained transgenic HIV/BYMV CP.
PCT/US2001/020272 2000-06-26 2001-06-26 Production of vaccines using transgenic plants WO2002000169A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002414396A CA2414396A1 (en) 2000-06-26 2001-06-26 Production of vaccines using transgenic plants
AU2001270164A AU2001270164A1 (en) 2000-06-26 2001-06-26 Production of vaccines using transgenic plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60399700A 2000-06-26 2000-06-26
US09/603,997 2000-06-26

Publications (2)

Publication Number Publication Date
WO2002000169A2 WO2002000169A2 (en) 2002-01-03
WO2002000169A3 true WO2002000169A3 (en) 2002-07-18

Family

ID=24417757

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/020272 WO2002000169A2 (en) 2000-06-26 2001-06-26 Production of vaccines using transgenic plants

Country Status (3)

Country Link
AU (1) AU2001270164A1 (en)
CA (1) CA2414396A1 (en)
WO (1) WO2002000169A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270164A1 (en) * 2000-06-26 2002-01-08 The United States Of America As Represented By The Secretary Of Agriculture Production of vaccines using transgenic plants
US8101189B2 (en) 2002-07-05 2012-01-24 Folia Biotech Inc. Vaccines and immunopotentiating compositions and methods for making and using them
EP1523329B1 (en) 2002-07-05 2013-07-24 Folia Biotech Inc. Adjuvant viral particle
DE602004028004D1 (en) * 2003-05-05 2010-08-19 Thompson Boyce Plant Res VECTORS AND CELLS FOR THE MANUFACTURE OF IMMUNOPROTEKTIVE COMPOSITIONS DERIVED FROM TRANSGENIC PLANTS
WO2005108564A2 (en) * 2004-03-25 2005-11-17 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusion
WO2005118806A2 (en) 2004-05-28 2005-12-15 Ambion, Inc. METHODS AND COMPOSITIONS INVOLVING MicroRNA
US20050287539A1 (en) * 2004-06-29 2005-12-29 Emmanuel Labourier Methods and compositions for preparing capped RNA
US20100047264A1 (en) 2006-11-15 2010-02-25 Folia Biotech Inc. Immunogenic Affinity-Conjugated Antigen Systems Based on Papaya Mosaic Virus and Uses Thereof
EP2198050A1 (en) 2007-09-14 2010-06-23 Asuragen, INC. Micrornas differentially expressed in cervical cancer and uses thereof
RU2370280C2 (en) * 2007-10-08 2009-10-20 Институт физико-химической биологии им. А.Н.Белозерского METHOD OF HER2/neu ONCOGENE ANTIBODY SUPERPRODUCTION IN PLANT
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
ATE539148T1 (en) 2009-11-30 2012-01-15 United Cancer Res Inst NEW CLONE OF AVIAN PLAGUE VIRUS, PREPARATION AND APPLICATION IN MEDICAL TREATMENT OF CANCER
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
CN108315493A (en) * 2018-04-23 2018-07-24 上海市农业科学院 A kind of LAMP primer group and detection method for detecting barley yellow mosaic virus
CN109486992B (en) * 2018-12-11 2021-08-20 贵州省烟草科学研究院 Specific co-dominant molecular marker for large-fragment deletion mutation of eIF4E-1 site of tobacco and application

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4970168A (en) * 1989-01-27 1990-11-13 Monsanto Company Virus-resistant plants
US5041385A (en) * 1986-11-01 1991-08-20 Oxford Gene Systems Limited Vector expressing fusion proteins and particles
WO1991015587A1 (en) * 1990-04-06 1991-10-17 Commonwealth Scientific And Industrial Research Organisation Self-polymerising expression system based on modified potyvirus coat proteins
WO1998056933A1 (en) * 1997-06-12 1998-12-17 John Innes Centre Polypeptide presentation system
US5874087A (en) * 1991-04-19 1999-02-23 Axis Genetics Plc Modified plant viruses as vectors
US5879925A (en) * 1994-08-15 1999-03-09 Connaught Laboratories Limited Genetically engineered human immunodeficiency virus-like particles with modified chimeric envelope glycoproteins containing influenza virus transmembrane spanning domains
US5998699A (en) * 1988-08-19 1999-12-07 Seminis Vegetable Seeds, Inc. Potyvirus coat protein genes and plants transformed therewith
US6232099B1 (en) * 1994-10-18 2001-05-15 Scottish Crop Research Institute Method of producing a chimeric protein
WO2002000169A2 (en) * 2000-06-26 2002-01-03 The United States Of America, As Represented By The Secretary Of Agriculture Production of vaccines using transgenic plants

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5041385A (en) * 1986-11-01 1991-08-20 Oxford Gene Systems Limited Vector expressing fusion proteins and particles
US5998699A (en) * 1988-08-19 1999-12-07 Seminis Vegetable Seeds, Inc. Potyvirus coat protein genes and plants transformed therewith
US4970168A (en) * 1989-01-27 1990-11-13 Monsanto Company Virus-resistant plants
WO1991015587A1 (en) * 1990-04-06 1991-10-17 Commonwealth Scientific And Industrial Research Organisation Self-polymerising expression system based on modified potyvirus coat proteins
US5874087A (en) * 1991-04-19 1999-02-23 Axis Genetics Plc Modified plant viruses as vectors
US5879925A (en) * 1994-08-15 1999-03-09 Connaught Laboratories Limited Genetically engineered human immunodeficiency virus-like particles with modified chimeric envelope glycoproteins containing influenza virus transmembrane spanning domains
US6232099B1 (en) * 1994-10-18 2001-05-15 Scottish Crop Research Institute Method of producing a chimeric protein
WO1998056933A1 (en) * 1997-06-12 1998-12-17 John Innes Centre Polypeptide presentation system
WO2002000169A2 (en) * 2000-06-26 2002-01-03 The United States Of America, As Represented By The Secretary Of Agriculture Production of vaccines using transgenic plants

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules

Also Published As

Publication number Publication date
AU2001270164A1 (en) 2002-01-08
CA2414396A1 (en) 2002-01-03
WO2002000169A2 (en) 2002-01-03

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