WO2000012677A3 - Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines - Google Patents

Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines Download PDF

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Publication number
WO2000012677A3
WO2000012677A3 PCT/IB1999/001604 IB9901604W WO0012677A3 WO 2000012677 A3 WO2000012677 A3 WO 2000012677A3 IB 9901604 W IB9901604 W IB 9901604W WO 0012677 A3 WO0012677 A3 WO 0012677A3
Authority
WO
WIPO (PCT)
Prior art keywords
viruses
generation
recombinant
strains
attenuated
Prior art date
Application number
PCT/IB1999/001604
Other languages
French (fr)
Other versions
WO2000012677A2 (en
Inventor
Boon-Leong Lim
Original Assignee
Univ Hong Kong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hong Kong filed Critical Univ Hong Kong
Publication of WO2000012677A2 publication Critical patent/WO2000012677A2/en
Publication of WO2000012677A3 publication Critical patent/WO2000012677A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The invention relates to the generation and mutagenesis of recombinant infectious bursal disease virus by reverse genetics technology. Site-directed mutagenesis of certain amino acid residues on the Non-CEF adapted, very virulent strains of IBDV transforms the viruses into attenuated, CEF-adapted strains. The attenuated, CEF-adapted strains can be used as live vaccines against the very virulent strains. Curently available live attenuated vaccines were generated by serial passage through cell culture and the like, whereas the present invention provides a novel molecular mechanism in manipulation of the viral genome and the generation of attenuated vaccines by site-directed mutagenesis. The same approach can be used to produce vaccine strains from newly evolved IBDV viruses or to manipulate the antigenicity and pathogenicity of the virus.
PCT/IB1999/001604 1998-09-01 1999-09-01 Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines WO2000012677A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9868498P 1998-09-01 1998-09-01
US60/098,684 1998-09-01

Publications (2)

Publication Number Publication Date
WO2000012677A2 WO2000012677A2 (en) 2000-03-09
WO2000012677A3 true WO2000012677A3 (en) 2000-06-29

Family

ID=22270458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001604 WO2000012677A2 (en) 1998-09-01 1999-09-01 Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines

Country Status (1)

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WO (1) WO2000012677A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1170302B1 (en) * 2000-07-07 2006-06-21 Intervet International BV Infectious bursal disease virus vaccines
US6485940B2 (en) 2000-07-07 2002-11-26 Akzo Nobel N.V. Broad spectrum infectious bursal disease virus vaccine
CN101123871B (en) * 2003-05-05 2011-05-18 美国陶氏益农公司 Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
ES2417019T3 (en) * 2003-11-13 2013-08-05 University Of Georgia Research Foundation, Inc. Characterization procedure of infectious bursitis disease virus
CN102258777B (en) * 2011-06-30 2012-09-26 河南农业大学禽病研究所 Method for preparing inactivated vaccine and combined vaccine by breeding infectious bursal disease virus (IBDV) by chicken embryo source cell line
EP3620505A1 (en) 2018-09-07 2020-03-11 Henkel AG & Co. KGaA Mixed metal colloids as bleach boosting agents
EP3620504A1 (en) 2018-09-07 2020-03-11 Henkel AG & Co. KGaA Platinum colloids as bleach boosting agents

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986007060A1 (en) * 1985-05-30 1986-12-04 Commonwealth Scientific And Industrial Research Or Cloning and expression of host-protective immunogens of ibdv
WO1991016925A1 (en) * 1990-05-04 1991-11-14 University Of Maryland At College Park Specific dna sequences related to an ibdv protein including vectors, hosts and vaccines
WO1995026196A1 (en) * 1994-03-29 1995-10-05 The University Of Maryland College Park CHIMERIC INFECTIOUS BURSAL DISEASE VIRUS cDNA CLONES, EXPRESSION PRODUCTS AND VACCINES BASED THEREON
WO1998009646A1 (en) * 1996-09-05 1998-03-12 University Of Maryland - Biotechnology Institute A method for generating birnavirus from synthetic rna transcripts

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986007060A1 (en) * 1985-05-30 1986-12-04 Commonwealth Scientific And Industrial Research Or Cloning and expression of host-protective immunogens of ibdv
WO1991016925A1 (en) * 1990-05-04 1991-11-14 University Of Maryland At College Park Specific dna sequences related to an ibdv protein including vectors, hosts and vaccines
WO1995026196A1 (en) * 1994-03-29 1995-10-05 The University Of Maryland College Park CHIMERIC INFECTIOUS BURSAL DISEASE VIRUS cDNA CLONES, EXPRESSION PRODUCTS AND VACCINES BASED THEREON
WO1998009646A1 (en) * 1996-09-05 1998-03-12 University Of Maryland - Biotechnology Institute A method for generating birnavirus from synthetic rna transcripts

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Publication number Publication date
WO2000012677A2 (en) 2000-03-09

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