WO2001092569A2 - Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci - Google Patents

Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci Download PDF

Info

Publication number
WO2001092569A2
WO2001092569A2 PCT/GB2001/002265 GB0102265W WO0192569A2 WO 2001092569 A2 WO2001092569 A2 WO 2001092569A2 GB 0102265 W GB0102265 W GB 0102265W WO 0192569 A2 WO0192569 A2 WO 0192569A2
Authority
WO
WIPO (PCT)
Prior art keywords
formulation
vessel
polymerase
chain reaction
effecting
Prior art date
Application number
PCT/GB2001/002265
Other languages
English (en)
Other versions
WO2001092569A3 (fr
Inventor
John Douglas Oultram
Conor Joseph Mulrooney
Jacqueline Clare Coutts
Original Assignee
Tepnel Medical Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0013043A external-priority patent/GB0013043D0/en
Priority claimed from GB0013863A external-priority patent/GB0013863D0/en
Application filed by Tepnel Medical Limited filed Critical Tepnel Medical Limited
Priority to EP01936610A priority Critical patent/EP1290223A2/fr
Priority to AU2001262483A priority patent/AU2001262483A1/en
Publication of WO2001092569A2 publication Critical patent/WO2001092569A2/fr
Publication of WO2001092569A3 publication Critical patent/WO2001092569A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Definitions

  • the present invention relates to formulations for use in effecting a Polymerase Chain Reaction and also to vessels containing such a formulation and which are intended for use in conducting such a reaction.
  • PCR Polymerase Chain Reaction
  • This lyophilised mixture has the advantage that it simplifies the multi-step PCR manipulation in that all components (except target) for effecting amplification are included in the pre-prepared mixture such that all that is required is addition of an aqueous sample containing (or potentially' containing) the target. Furthermore, the water soluble dye facilitates identification of complete mixing of the PCR reagent and test sample and saves the trouble of adding a sample loading buffer which is otherwise required for analysis of PCR products. As a result, the formulations of US-A-5 861 251 provide the advantage of avoiding carry-over contamination into the PCR reaction mix. However detection of the amplified product is effected by running the product mixture on a gel. This necessities opening of the tube, to apply the product mixture to the gel, thus once again giving rise to the possibility of cross-contamination.
  • a formulation for use in effecting a Polymerase Chain Reaction comprising a dried composition of reagents including reaction buffer, dNTPs, at least two primers and a polymerase and said formulation being re-hydratable to be capable of effecting amplification of a target nucleic acid sequence of interest characterised in that the formulation incorporates a fluorescent reporter molecule capable of reporting by homologous detection the presence of amplified nucleic acid produced by the Polymerase Chain Reaction..
  • the formulation of the invention is such that only a single addition of aqueous target sample to the formulation is required to produce an aqueous reaction mixture containing all necessary components for PCR amplification of target nucleic acid sequence.
  • the formulation of the invention does however have the significant additional advantage that the presence, in the formulation, of the fluorescent reporter molecule means homologous detection may be used.
  • the progress of the reaction may be followed by real-time detection techniques avoiding the need for post-reaction manipulation of the product mixture (e.g. transferring the mixture to a gel, or even opening a vessel in which the product mixture is contained) thereby avoiding any possibility of cross-contamination.
  • This has dramatic consequences for the set-up of laboratories that PERform PCR-based diagnostic reactions as, currently, extreme care has to be taken during the performance of the reaction to prevent cross-contamination.
  • no particular contamination controls would be needed other than those routine in a molecular biology laboratory.
  • There are also additional benefits including having much more defined reaction conditions (as essentially all the reactants could come from the same batch, convenience, longer shelf life etc).
  • the invention also provides, according to a second aspect thereof, a vessel (e.g. a reaction tube) containing a pre-measured amount of the formulation of the invention.
  • a vessel e.g. a reaction tube
  • the vessels may be provided with a suitable closure element and supplied to end users who, after removal of the closure element merely, need only to add the aqueous sample and then re-close the vessel.
  • the end user may be a person in a laboratory where the PCR reaction is then effected. Alternatively the end user may be out "on-site" collecting samples which can then be added to the vessel as soon as collected, the vessel then being sent to a laboratory for conducting the PCR reaction.
  • the inner surface of the vessel (adjacent the mouth thereof) and the outer surface of the closure element may be provided with inter-engageable formations allowing insertion of the closure element into the vessel but preventing withdrawal therefrom.
  • inter-engagable formations should be positioned such that the closure element is capable of being removable provided that it has not been inserted into the vessel beyond a certain degree.
  • the dried composition may be incorporated into the vessel and the closure element removably applied thereto. Subsequently the closure element may be removed to permit addition of the sample and then subsequently inserted sufficiently far into the vessel so that it becomes non-removable.
  • the formulation of the invention may be prepared by lyophilisation of an aqueous solution of the required components, e.g. by lyophilisation using the procedures disclosed in US-A-5 861 251.
  • the solution includes a stabiliser which may for example be glucose, glucitol or trehalose.
  • the dried formulation of the invention may be such that, per ml of reconstituted reaction medium, it comprises: Component Amount
  • Stabiliser e.g. trehalose 0.1-15% w/w
  • the PCR reaction may be conducted by procedures well known in the art, e.g. using thermal cycling.
  • the fluorescent reporter molecule included in the formulation of the invention may for example be one which reports a change in the amount of double stranded DNA present in the reaction, e.g. an intercalating dye such as Ethidium Bromide, CyBr- Green or PicoGreen.
  • the fluorescent reporter molecule may be one which works in conjunction with a quencher moiety so as to be capable of reporting on the presence of specific nucleotide sequences in the mixture and may, for example, be a TaqMan probe, Molecular Beacon, Sunrise primer and Scorpion primer (Registered Trade Marks).
  • the polymerase may be a DNA polymerase and may be a thermally stable polymerase, e.g. Taq polymerase.
  • Taq polymerase e.g. a thermally stable polymerase
  • the polymerase in the formulation of the invention is a "hot-start" polymerase.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une formulation s'utilisant pour mettre en oeuvre une amplification en chaîne par polymérase, une composition séchée de réactifs renfermant un tampon de réaction, des dNTP, au moins deux amorces et une polymérase. La formulation est réhydratable de manière à permettre l'amplification d'une séquence d'acides nucléiques voulue. La formulation incorpore une molécule rapporteur fluorescente capable de rapporter par détection homologue la présence d'acide nucléique amplifié, produit par l'amplification en chaîne par polymérase.
PCT/GB2001/002265 2000-05-31 2001-05-23 Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci WO2001092569A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01936610A EP1290223A2 (fr) 2000-05-31 2001-05-23 Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci
AU2001262483A AU2001262483A1 (en) 2000-05-31 2001-05-23 Formulation for polymerase chain reaction and vessel containing same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0013043.5 2000-05-31
GB0013043A GB0013043D0 (en) 2000-05-31 2000-05-31 Formulation for polymerase chain reaction and vessel containing same
GB0013863.6 2000-06-07
GB0013863A GB0013863D0 (en) 2000-06-07 2000-06-07 Formulation for polymerase chain reaction and vessel containing same

Publications (2)

Publication Number Publication Date
WO2001092569A2 true WO2001092569A2 (fr) 2001-12-06
WO2001092569A3 WO2001092569A3 (fr) 2002-03-28

Family

ID=26244374

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002265 WO2001092569A2 (fr) 2000-05-31 2001-05-23 Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci

Country Status (3)

Country Link
EP (1) EP1290223A2 (fr)
AU (1) AU2001262483A1 (fr)
WO (1) WO2001092569A2 (fr)

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1350855A2 (fr) * 2002-04-01 2003-10-08 Agilent Technologies, Inc. - a Delaware corporation - Tests d'hybridation reposant sur un système en rangées (array)
EP1498492A1 (fr) * 2003-07-15 2005-01-19 Lukas Bestmann Appareil pour la préparation d'échantillons
WO2005118849A1 (fr) * 2004-06-04 2005-12-15 Abacus Diagnostica Oy Procédé de stabilisation des réactifs de test, les conteneurs de réactifs avec des réactifs de test stabilisés et leur utilisation
WO2006000648A1 (fr) * 2004-06-29 2006-01-05 Wallac Oy Analyse d'acide nucleique integree
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
EP2210955A3 (fr) * 2006-05-23 2010-09-15 Molecular Detection, Inc. Kits de température ambiante stable pour diagnostics moléculaires
WO2012010708A1 (fr) * 2010-07-23 2012-01-26 Aj Innuscreen Gmbh Procédé, dispositif et kit d'analyse pour réactions de biologie moléculaire
EP2574931A1 (fr) 2011-09-29 2013-04-03 Qiagen GmbH Composition sèche comprenant un colorant de contrôle
WO2013053855A1 (fr) 2011-10-11 2013-04-18 Qiagen Gmbh Procédé de traitement d'échantillon et cartouche de traitement d'échantillon
WO2013068107A1 (fr) 2011-11-07 2013-05-16 Qiagen Gmbh Méthode de lyse et composition de lyse
EP2730653A1 (fr) 2012-11-07 2014-05-14 QIAGEN GmbH Procédé de lyse d'un échantillon biologique fixe
US10875022B2 (en) 2007-07-13 2020-12-29 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10913061B2 (en) 2006-03-24 2021-02-09 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US11060082B2 (en) 2007-07-13 2021-07-13 Handy Lab, Inc. Polynucleotide capture materials, and systems using same
US11078523B2 (en) 2003-07-31 2021-08-03 Handylab, Inc. Processing particle-containing samples
US11141734B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11142785B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11266987B2 (en) 2007-07-13 2022-03-08 Handylab, Inc. Microfluidic cartridge
US11441171B2 (en) 2004-05-03 2022-09-13 Handylab, Inc. Method for processing polynucleotide-containing samples
US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
US11466263B2 (en) 2007-07-13 2022-10-11 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
US11549959B2 (en) 2007-07-13 2023-01-10 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11788127B2 (en) 2011-04-15 2023-10-17 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (fr) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Procédé pour la détection d'ADN dans un échantillon
EP0674009A2 (fr) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Procédé et dispositif d'amplification d'acide nucléique
WO1997010056A2 (fr) * 1995-09-12 1997-03-20 Becton Dickinson And Company Dispositif et procede d'amplification et de dosage d'adn
EP0834729A2 (fr) * 1996-09-26 1998-04-08 Becton, Dickinson and Company Plaque à puits multiples et méthode d'analyse pour ADN
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (fr) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Procédé pour la détection d'ADN dans un échantillon
EP0674009A2 (fr) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Procédé et dispositif d'amplification d'acide nucléique
WO1997010056A2 (fr) * 1995-09-12 1997-03-20 Becton Dickinson And Company Dispositif et procede d'amplification et de dosage d'adn
EP0834729A2 (fr) * 1996-09-26 1998-04-08 Becton, Dickinson and Company Plaque à puits multiples et méthode d'analyse pour ADN
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction

Cited By (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1350855A2 (fr) * 2002-04-01 2003-10-08 Agilent Technologies, Inc. - a Delaware corporation - Tests d'hybridation reposant sur un système en rangées (array)
EP1350855A3 (fr) * 2002-04-01 2003-11-19 Agilent Technologies, Inc. - a Delaware corporation - Tests d'hybridation reposant sur un système en rangées (array)
EP1498492A1 (fr) * 2003-07-15 2005-01-19 Lukas Bestmann Appareil pour la préparation d'échantillons
WO2005007882A2 (fr) * 2003-07-15 2005-01-27 Dual, Jürg Unite de preparation d'echantillons
WO2005007882A3 (fr) * 2003-07-15 2005-05-19 Lukas Bestmann Unite de preparation d'echantillons
US11078523B2 (en) 2003-07-31 2021-08-03 Handylab, Inc. Processing particle-containing samples
US11441171B2 (en) 2004-05-03 2022-09-13 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2005118849A1 (fr) * 2004-06-04 2005-12-15 Abacus Diagnostica Oy Procédé de stabilisation des réactifs de test, les conteneurs de réactifs avec des réactifs de test stabilisés et leur utilisation
JP2008501331A (ja) * 2004-06-04 2008-01-24 アバクス ディアグノスティカ オサケ ユキチュア アッセイ試薬の安定化方法、安定化アッセイ試薬を収容した試薬容器およびその使用
US7972838B2 (en) 2004-06-04 2011-07-05 Abacus Diagnostica Oy Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
WO2006000648A1 (fr) * 2004-06-29 2006-01-05 Wallac Oy Analyse d'acide nucleique integree
US8252536B2 (en) 2004-06-29 2012-08-28 Wallac Oy Integrated nucleic acid analysis
US11666903B2 (en) 2006-03-24 2023-06-06 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11959126B2 (en) 2006-03-24 2024-04-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11142785B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11141734B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11085069B2 (en) 2006-03-24 2021-08-10 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10913061B2 (en) 2006-03-24 2021-02-09 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
EP2210955A3 (fr) * 2006-05-23 2010-09-15 Molecular Detection, Inc. Kits de température ambiante stable pour diagnostics moléculaires
US11549959B2 (en) 2007-07-13 2023-01-10 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11254927B2 (en) 2007-07-13 2022-02-22 Handylab, Inc. Polynucleotide capture materials, and systems using same
US11266987B2 (en) 2007-07-13 2022-03-08 Handylab, Inc. Microfluidic cartridge
US10875022B2 (en) 2007-07-13 2020-12-29 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11466263B2 (en) 2007-07-13 2022-10-11 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
US11060082B2 (en) 2007-07-13 2021-07-13 Handy Lab, Inc. Polynucleotide capture materials, and systems using same
WO2012010708A1 (fr) * 2010-07-23 2012-01-26 Aj Innuscreen Gmbh Procédé, dispositif et kit d'analyse pour réactions de biologie moléculaire
US11788127B2 (en) 2011-04-15 2023-10-17 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
EP2574931A1 (fr) 2011-09-29 2013-04-03 Qiagen GmbH Composition sèche comprenant un colorant de contrôle
WO2013053855A1 (fr) 2011-10-11 2013-04-18 Qiagen Gmbh Procédé de traitement d'échantillon et cartouche de traitement d'échantillon
CN104066849A (zh) * 2011-10-11 2014-09-24 凯杰有限公司 样品处理方法和样品处理盒
EP3663408A1 (fr) 2011-10-11 2020-06-10 QIAGEN GmbH Procédé de traitement d'échantillons et cartouche de traitement d'échantillons
CN104066849B (zh) * 2011-10-11 2017-04-19 凯杰有限公司 样品处理方法和样品处理盒
US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
WO2013068107A1 (fr) 2011-11-07 2013-05-16 Qiagen Gmbh Méthode de lyse et composition de lyse
EP2730653A1 (fr) 2012-11-07 2014-05-14 QIAGEN GmbH Procédé de lyse d'un échantillon biologique fixe
WO2014072366A1 (fr) 2012-11-07 2014-05-15 Qiagen Gmbh Procédé de lyse d'un échantillon biologique fixé

Also Published As

Publication number Publication date
EP1290223A2 (fr) 2003-03-12
AU2001262483A1 (en) 2001-12-11
WO2001092569A3 (fr) 2002-03-28

Similar Documents

Publication Publication Date Title
WO2001092569A2 (fr) Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci
US9476092B2 (en) Late-PCR
US9487807B2 (en) Compositions and methods for producing single-stranded circular DNA
US7273730B2 (en) Nested PCR employing degradable primers
US20180094306A1 (en) Nucleic Acid Amplification
EP2324124B1 (fr) Correction d'épreuve d'extension d'amorce
CA2706444C (fr) Inhibition reduite d'acp de rt en une seule etape
Wittwer Rapid cycle real-time PCR: methods and applications
Lee et al. Fluorescent detection techniques for real‐time multiplex strand specific detection of Bacillus anthracis using rapid PCR
WO2010019898A1 (fr) Procédé de détection d'acide nucléique à température contrôlée adapté pour être mis en pratique dans un système fermé
US11254960B2 (en) Nucleic acid amplification
McChlery et al. The use of hydrolysis and hairpin probes in real-time PCR
CA3036758C (fr) Compositions, dispositifs et procedes de controle interne de phi6
JP6174999B2 (ja) Ｐｃｒ反応緩衝液中での細胞溶解のための方法
EP1942196A2 (fr) Late-pcr
Tayyeb et al. Polymerase Chain Reaction
Sorscher DNA amplification techniques: An overview
CN110621783B (zh) 多重核酸扩增测定
CA3232224A1 (fr) Amorce en boucle avec diverses modifications internes et procede de boucle-de-boucle pour la detection de cible
Thomas Polymerase chain reaction and its various modifications In: Winter School on Vistas in Marine Biotechnology 5th to 26th October 2010

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2001936610

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001936610

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2001936610

Country of ref document: EP