WO2001090387A3 - Gene regulatory region that promotes root-specific transcription and its uses - Google Patents

Gene regulatory region that promotes root-specific transcription and its uses Download PDF

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Publication number
WO2001090387A3
WO2001090387A3 PCT/IB2001/001137 IB0101137W WO0190387A3 WO 2001090387 A3 WO2001090387 A3 WO 2001090387A3 IB 0101137 W IB0101137 W IB 0101137W WO 0190387 A3 WO0190387 A3 WO 0190387A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific transcription
regulatory region
plants
gene regulatory
sequence
Prior art date
Application number
PCT/IB2001/001137
Other languages
French (fr)
Other versions
WO2001090387A2 (en
Inventor
Ljerka Kunst
Mark Andrew Smith
Hangsik Moon
Original Assignee
Univ British Columbia
Ljerka Kunst
Mark Andrew Smith
Hangsik Moon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ British Columbia, Ljerka Kunst, Mark Andrew Smith, Hangsik Moon filed Critical Univ British Columbia
Priority to CA002409881A priority Critical patent/CA2409881A1/en
Priority to AU2001274405A priority patent/AU2001274405A1/en
Priority to BR0111081-0A priority patent/BR0111081A/en
Priority to EP01940917A priority patent/EP1283893A2/en
Publication of WO2001090387A2 publication Critical patent/WO2001090387A2/en
Publication of WO2001090387A3 publication Critical patent/WO2001090387A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8227—Root-specific

Abstract

Nucleic acid sequence which directs root-specific transcription of contiguous genes in plants is provided. This sequence may be used in transgenic plants to promote expression of endogenous and foreign genes in roots to enhance plant resistance to diseases and pests, stress (salt) tolerance, and improve nutritive value of edible root plants. In addition, this sequence may be useful for the production of recombinant proteins in roots aimed at molecular farming and phytoremediation.
PCT/IB2001/001137 2000-05-24 2001-05-24 Gene regulatory region that promotes root-specific transcription and its uses WO2001090387A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002409881A CA2409881A1 (en) 2000-05-24 2001-05-24 Gene regulatory region that promotes root-specific transcription and its uses
AU2001274405A AU2001274405A1 (en) 2000-05-24 2001-05-24 Gene regulatory region that promotes root-specific transcription and its uses
BR0111081-0A BR0111081A (en) 2000-05-24 2001-05-24 Gene regulatory region that specifically promotes root transcription and uses
EP01940917A EP1283893A2 (en) 2000-05-24 2001-05-24 Gene regulatory region that promotes root-specific transcription and its uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20678800P 2000-05-24 2000-05-24
US60/206,788 2000-05-24

Publications (2)

Publication Number Publication Date
WO2001090387A2 WO2001090387A2 (en) 2001-11-29
WO2001090387A3 true WO2001090387A3 (en) 2002-06-27

Family

ID=22767963

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/001137 WO2001090387A2 (en) 2000-05-24 2001-05-24 Gene regulatory region that promotes root-specific transcription and its uses

Country Status (5)

Country Link
EP (1) EP1283893A2 (en)
AU (1) AU2001274405A1 (en)
BR (1) BR0111081A (en)
CA (1) CA2409881A1 (en)
WO (1) WO2001090387A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007357A2 (en) * 1993-09-04 1995-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Promoters
WO1997005261A1 (en) * 1995-07-28 1997-02-13 North Carolina State University Root cortex specific gene promoter
EP0824150A2 (en) * 1996-08-12 1998-02-18 Sumitomo Chemical Company, Limited Plant promoter and utilization thereof
WO1998046766A1 (en) * 1997-04-14 1998-10-22 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
WO1999053016A2 (en) * 1998-04-09 1999-10-21 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Expression of alfin1 and methods for producing transgenic plants having increased root growth and root specific gene activation
WO1999054471A1 (en) * 1998-04-20 1999-10-28 Zeneca Limited Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata
WO2001090364A2 (en) * 2000-05-24 2001-11-29 The University Of British Columbia Nucleic acid encoding a plant very long chain fatty acid biosynthetic enzyme

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007357A2 (en) * 1993-09-04 1995-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Promoters
WO1997005261A1 (en) * 1995-07-28 1997-02-13 North Carolina State University Root cortex specific gene promoter
EP0824150A2 (en) * 1996-08-12 1998-02-18 Sumitomo Chemical Company, Limited Plant promoter and utilization thereof
WO1998046766A1 (en) * 1997-04-14 1998-10-22 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
WO1999053016A2 (en) * 1998-04-09 1999-10-21 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Expression of alfin1 and methods for producing transgenic plants having increased root growth and root specific gene activation
WO1999054471A1 (en) * 1998-04-20 1999-10-28 Zeneca Limited Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata
WO2001090364A2 (en) * 2000-05-24 2001-11-29 The University Of British Columbia Nucleic acid encoding a plant very long chain fatty acid biosynthetic enzyme

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 26 September 1999 (1999-09-26), CARDON, G.H.: "Arabidopsis thaliana (ecotype Landsberg erecta) mRNA for squamosa promoter binding protein-like 1", XP002195417 *
DEVIC MARTINE ET AL: "Efficient PCR walking on plant genomic DNA", PLANT PHYSIOLOGY AND BIOCHEMISTRY, GAUTHIER-VILLARS, PARIS, FR, vol. 35, no. 4, 1997, pages 331 - 339, XP000926417, ISSN: 0981-9428 *
SCHRODER-PONTOPPIDAN M ET AL: "Very long chain and hydroxylated fatty acids in offspring of somatic hybrids between Brassica napus and Lesquerella fendleri.", THEORETICAL AND APPLIED GENETICS, vol. 99, no. 1-2, July 1999 (1999-07-01), pages 108 - 114, XP002195215, ISSN: 0040-5752 *

Also Published As

Publication number Publication date
BR0111081A (en) 2003-04-08
CA2409881A1 (en) 2001-11-29
EP1283893A2 (en) 2003-02-19
WO2001090387A2 (en) 2001-11-29
AU2001274405A1 (en) 2001-12-03

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