WO2001077374A3 - Verfahren zur identifizierung und isolierung von genomfragmenten mit kopplungsungleichgewicht - Google Patents

Verfahren zur identifizierung und isolierung von genomfragmenten mit kopplungsungleichgewicht Download PDF

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Publication number
WO2001077374A3
WO2001077374A3 PCT/DE2001/001488 DE0101488W WO0177374A3 WO 2001077374 A3 WO2001077374 A3 WO 2001077374A3 DE 0101488 W DE0101488 W DE 0101488W WO 0177374 A3 WO0177374 A3 WO 0177374A3
Authority
WO
WIPO (PCT)
Prior art keywords
linkage disequilibrium
identifying
methods
genome fragments
carried out
Prior art date
Application number
PCT/DE2001/001488
Other languages
English (en)
French (fr)
Other versions
WO2001077374A2 (de
Inventor
Klaus Olek
Juergen Weber
Thomas Jansen
Marco Leuer
Original Assignee
Biopsytek Analytik Gmbh
Klaus Olek
Juergen Weber
Thomas Jansen
Marco Leuer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biopsytek Analytik Gmbh, Klaus Olek, Juergen Weber, Thomas Jansen, Marco Leuer filed Critical Biopsytek Analytik Gmbh
Priority to AU2001273842A priority Critical patent/AU2001273842A1/en
Priority to NZ522470A priority patent/NZ522470A/xx
Priority to EP01940160A priority patent/EP1272674A2/de
Priority to JP2001575228A priority patent/JP2003530117A/ja
Priority to CA002416118A priority patent/CA2416118A1/en
Publication of WO2001077374A2 publication Critical patent/WO2001077374A2/de
Publication of WO2001077374A3 publication Critical patent/WO2001077374A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Gegenstand der Erfindung ist ein Verfahren zur Identifizierung und Isolierung von Genomfragmenten mit Kopplungsungleichgewicht, wobei man bei nicht miteinander verwandten sowie bei miteinander verwandten Individuen Bereiche des Genoms isoliert, welche Kandidatengenbereiche enthalten, die sich im Kopplungsungleichgewicht mit ihrer engeren DNA-Umgebung befinden. Die im Vergleich zu anderen Verfahren frühere Durchführung des Klonierungsschritts ermöglicht eine mengenunabhängige Gewinnung der DNA-Fragmente und ersetzt weiterhin den in anderen Verfahren durchgeführten Methylierungsschritt. Außerdem wurde gefunden, dass die erfindungsgemäße Verwendung eines Pflanzenenzym eine weitaus höhere Spezifität des Verfahrens gegenüber solchen bewirkt, bei denen der Mut S,H,L-Komplex verwendet wird.
PCT/DE2001/001488 2000-04-08 2001-04-08 Verfahren zur identifizierung und isolierung von genomfragmenten mit kopplungsungleichgewicht WO2001077374A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2001273842A AU2001273842A1 (en) 2000-04-08 2001-04-08 Method for identifying and isolating genome fragments with linkage disequilibrium
NZ522470A NZ522470A (en) 2000-04-08 2001-04-08 Method for identifying and isolating genome fragments with linkage disequilibrium involving the conduction of an early cloning step
EP01940160A EP1272674A2 (de) 2000-04-08 2001-04-08 Verfahren zur identifizierung und isolierung von genomfragmenten mit kopplungsungleichgewicht
JP2001575228A JP2003530117A (ja) 2000-04-08 2001-04-08 ゲノム−断片の結合不等重量による同定及び分離方法
CA002416118A CA2416118A1 (en) 2000-04-08 2001-04-08 Method for identifying and isolating genome fragments with linkage disequilibrium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10017675.5 2000-04-08
DE10017675A DE10017675A1 (de) 2000-04-08 2000-04-08 Verfahren zur Identifizierung und Isolierung von Genomfragmenten mit Kopplungsungleichgewicht

Publications (2)

Publication Number Publication Date
WO2001077374A2 WO2001077374A2 (de) 2001-10-18
WO2001077374A3 true WO2001077374A3 (de) 2002-06-20

Family

ID=7638162

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001488 WO2001077374A2 (de) 2000-04-08 2001-04-08 Verfahren zur identifizierung und isolierung von genomfragmenten mit kopplungsungleichgewicht

Country Status (8)

Country Link
US (1) US20030165926A1 (de)
EP (1) EP1272674A2 (de)
JP (1) JP2003530117A (de)
AU (1) AU2001273842A1 (de)
CA (1) CA2416118A1 (de)
DE (1) DE10017675A1 (de)
NZ (1) NZ522470A (de)
WO (1) WO2001077374A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1529119A2 (de) * 2002-08-09 2005-05-11 Förderverein Biotechnologieforschung der deutschen Schweineproduktion e.V. Genetische marker fur die diagnose der pradisposition zur vererbung oder ausprägung des phanotyps "afterlosigkeit" bei haus-, zucht- und nutztieren
US20080228699A1 (en) 2007-03-16 2008-09-18 Expanse Networks, Inc. Creation of Attribute Combination Databases
EP2370929A4 (de) 2008-12-31 2016-11-23 23Andme Inc Suche nach verwandten in einer datenbank
CN109346130B (zh) * 2018-10-24 2021-10-08 中国科学院水生生物研究所 一种直接从全基因组重测序数据中得到微单体型及其分型的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001526A1 (en) * 1987-08-07 1989-02-23 Genelabs Incorporated Coincidence cloning method and library
WO1993022462A1 (en) * 1992-05-06 1993-11-11 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
WO2000053802A1 (en) * 1999-03-12 2000-09-14 Integragen, S.A.S. Compositions and methods for genetic analysis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6524794B1 (en) * 1999-10-29 2003-02-25 Decode Genetics Ehf. Identical-by-descent fragment enrichment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001526A1 (en) * 1987-08-07 1989-02-23 Genelabs Incorporated Coincidence cloning method and library
WO1993022462A1 (en) * 1992-05-06 1993-11-11 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
WO2000053802A1 (en) * 1999-03-12 2000-09-14 Integragen, S.A.S. Compositions and methods for genetic analysis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEUNG V G ET AL: "GENOMIC MISMATCH SCANNING IDENTIFIES HUMAN GENOMIC DNA SHARED IDENTICAL BY DESCENT", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 47, 1998, pages 1 - 6, XP000929953, ISSN: 0888-7543 *
MCALLISTER L ET AL: "ENRICHMENT FOR LOCI IDENTICAL-BY-DESCENT BETWEEN PAIRS OF MOUSE OR HUMAN GENOMES BY GENOMIC MISMATCH SCANNING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 47, no. 1, 1998, pages 7 - 11, XP000929905, ISSN: 0888-7543 *
NELSON S F ET AL: "GENOMIC MISMATCH SCANNING: A NEW APPROACH TO GENETIC LINKAGE MAPPING", NATURE GENETICS, NEW YORK, NY, US, vol. 4, no. 1, 1 May 1993 (1993-05-01), pages 11 - 17, XP000606264, ISSN: 1061-4036 *

Also Published As

Publication number Publication date
WO2001077374A2 (de) 2001-10-18
JP2003530117A (ja) 2003-10-14
AU2001273842A1 (en) 2001-10-23
NZ522470A (en) 2004-12-24
CA2416118A1 (en) 2001-10-18
EP1272674A2 (de) 2003-01-08
DE10017675A1 (de) 2001-12-06
US20030165926A1 (en) 2003-09-04

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