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WO2001061307A3 - Gel manipulation apparatus - Google Patents

Gel manipulation apparatus Download PDF

Info

Publication number
WO2001061307A3
WO2001061307A3 PCT/US2001/004709 US0104709W WO0161307A3 WO 2001061307 A3 WO2001061307 A3 WO 2001061307A3 US 0104709 W US0104709 W US 0104709W WO 0161307 A3 WO0161307 A3 WO 0161307A3
Authority
WO
WIPO (PCT)
Prior art keywords
gel
tank
assembly
carrier
robotic
Prior art date
Application number
PCT/US2001/004709
Other languages
French (fr)
Other versions
WO2001061307A2 (en
Inventor
N Leigh Anderson
Jack Goodman
Norman Anderson
Original Assignee
Large Scale Proteomics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/504,493 priority Critical patent/US6298874B1/en
Priority to US50449400A priority
Priority to US50449200A priority
Priority to US09/504,494 priority
Priority to US09/504,492 priority
Priority to US09/504,493 priority
Priority to US26021601P priority
Priority to US60/260,216 priority
Application filed by Large Scale Proteomics Corp filed Critical Large Scale Proteomics Corp
Publication of WO2001061307A2 publication Critical patent/WO2001061307A2/en
Publication of WO2001061307A3 publication Critical patent/WO2001061307A3/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44782Apparatus specially adapted therefor of a plurality of samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories

Abstract

An automated computer controlled apparatus (10) includes a robotic assembly (12) for capturing an electrophoresis gel (14) and transferring the gel to a sequence of treatment stages. The robotic assembly (12) has an articulated arm assembly for capturing a gel (14) and moving the gel to a selected work station. The gels (14) are supported by a carrier (106) having magnetically biased clamping jaws. The robotic arm assembly (12) is constructed to capture the carrier (106) to manipulate the gel. The gel (14) is suspended by the carrier (106) in a manner to prevent tearing and stretching of the gel. The gel treating stations have tanks (16) with surfaces to inhibit the gel from adhering to the walls of the tank. Typically, the surfaces of the walls have a plurality of channels formed by projections to allow the flow of liquid between the gel and the wall surface and reduce surface area of the wall contacting the gel. An agitating assembly (204) reciprocates the gel in the tank to agitate the treating liquid. In one embodiment, an agitating plate (374) moves in a reciprocating manner within the tank and is able to press the gel against a transparent wall of the tank at selected intervals so that an image of the gel can be captured at selected time intervals.
PCT/US2001/004709 2000-02-15 2001-02-15 Gel manipulation apparatus WO2001061307A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US50449400A true 2000-02-15 2000-02-15
US50449200A true 2000-02-15 2000-02-15
US09/504,494 2000-02-15
US09/504,492 2000-02-15
US09/504,493 2000-02-15
US09/504,493 US6298874B1 (en) 2000-02-15 2000-02-15 Slab gel processing tank
US26021601P true 2001-01-09 2001-01-09
US60/260,216 2001-01-09

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA 2400316 CA2400316A1 (en) 2000-02-15 2001-02-15 Gel manipulation apparatus
EP20010910658 EP1355846A2 (en) 2000-02-15 2001-02-15 Gel manipulation apparatus
AU3824301A AU3824301A (en) 2000-02-15 2001-02-15 Gel manipulation apparatus
JP2001560650A JP2004524509A (en) 2000-02-15 2001-02-15 Gel operating device

Publications (2)

Publication Number Publication Date
WO2001061307A2 WO2001061307A2 (en) 2001-08-23
WO2001061307A3 true WO2001061307A3 (en) 2003-04-03

Family

ID=27500669

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004709 WO2001061307A2 (en) 2000-02-15 2001-02-15 Gel manipulation apparatus

Country Status (5)

Country Link
EP (1) EP1355846A2 (en)
JP (1) JP2004524509A (en)
AU (1) AU3824301A (en)
CA (1) CA2400316A1 (en)
WO (1) WO2001061307A2 (en)

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2541448A (en) * 1945-09-21 1951-02-13 Edward T Verzal Venetian blind cleaner
US3888758A (en) * 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4178119A (en) * 1977-05-18 1979-12-11 Gerhard Busch Arrangement for turning, multiple stacking and aligning a paper stack
US4288904A (en) * 1978-03-03 1981-09-15 Svedala-Arbra Ab Method and apparatus for the reversal or replacement of hammers in hammer mills
US4594064A (en) * 1983-11-10 1986-06-10 The United States Of America As Represented By The United States Department Of Energy Automated apparatus for producing gradient gels
US4702814A (en) * 1986-09-15 1987-10-27 Center For Blood Research Non-mechanical buffer circulation apparatus for electrophoresis
US5028142A (en) * 1989-04-06 1991-07-02 Biotrack, Inc. Reciprocal mixer
US5073246A (en) * 1990-05-16 1991-12-17 Bio-Rad Laboratories, Inc. Slab electrophoresis system with improved sample wells and cooling mechanism
US5344534A (en) * 1993-03-05 1994-09-06 Danziger Robert S Gel electrophoresis measurement of dynamic reaction product by continuous sampling
US5458749A (en) * 1994-05-02 1995-10-17 Univeristy Of Iowa Research Foundation Device and process for staining electrophoretic gels
US5709838A (en) * 1992-06-01 1998-01-20 Nik Public Safety, Inc. Single use sampling device
JPH10147360A (en) * 1996-11-18 1998-06-02 Central Glass Co Ltd Spacer for loading glass sheet, method for mounting spacer, and its apparatus
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6090255A (en) * 1998-10-23 2000-07-18 Riley; Mary S. Vacuum package for electrophoresis gels

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2541448A (en) * 1945-09-21 1951-02-13 Edward T Verzal Venetian blind cleaner
US3888758A (en) * 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4178119A (en) * 1977-05-18 1979-12-11 Gerhard Busch Arrangement for turning, multiple stacking and aligning a paper stack
US4288904A (en) * 1978-03-03 1981-09-15 Svedala-Arbra Ab Method and apparatus for the reversal or replacement of hammers in hammer mills
US4594064A (en) * 1983-11-10 1986-06-10 The United States Of America As Represented By The United States Department Of Energy Automated apparatus for producing gradient gels
US4702814A (en) * 1986-09-15 1987-10-27 Center For Blood Research Non-mechanical buffer circulation apparatus for electrophoresis
US5028142A (en) * 1989-04-06 1991-07-02 Biotrack, Inc. Reciprocal mixer
US5073246A (en) * 1990-05-16 1991-12-17 Bio-Rad Laboratories, Inc. Slab electrophoresis system with improved sample wells and cooling mechanism
US5709838A (en) * 1992-06-01 1998-01-20 Nik Public Safety, Inc. Single use sampling device
US5344534A (en) * 1993-03-05 1994-09-06 Danziger Robert S Gel electrophoresis measurement of dynamic reaction product by continuous sampling
US5458749A (en) * 1994-05-02 1995-10-17 Univeristy Of Iowa Research Foundation Device and process for staining electrophoretic gels
JPH10147360A (en) * 1996-11-18 1998-06-02 Central Glass Co Ltd Spacer for loading glass sheet, method for mounting spacer, and its apparatus
US6182940B1 (en) * 1996-11-18 2001-02-06 Central Glass Co., Ltd. Spacer for stacking glass plates, method for attaching the spacer to the glass plate and apparatus for attaching the spacer to the glass plate
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6090255A (en) * 1998-10-23 2000-07-18 Riley; Mary S. Vacuum package for electrophoresis gels

Also Published As

Publication number Publication date
WO2001061307A2 (en) 2001-08-23
CA2400316A1 (en) 2001-08-23
AU3824301A (en) 2001-08-27
EP1355846A2 (en) 2003-10-29
JP2004524509A (en) 2004-08-12

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