WO2001059131A3 - Verwendung von palatinase- und trehalulase-sequenzen als nutritive marker in transformierten zellen - Google Patents

Verwendung von palatinase- und trehalulase-sequenzen als nutritive marker in transformierten zellen Download PDF

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Publication number
WO2001059131A3
WO2001059131A3 PCT/EP2001/001602 EP0101602W WO0159131A3 WO 2001059131 A3 WO2001059131 A3 WO 2001059131A3 EP 0101602 W EP0101602 W EP 0101602W WO 0159131 A3 WO0159131 A3 WO 0159131A3
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WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
relates
hydrolase
plants
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PCT/EP2001/001602
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English (en)
French (fr)
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WO2001059131A2 (de
Inventor
Frederik Boernke
Uwe Sonnewald
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Ipk Inst Fuer Pflanzengenetik
Frederik Boernke
Uwe Sonnewald
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Publication date
Priority claimed from DE10045182A external-priority patent/DE10045182A1/de
Application filed by Ipk Inst Fuer Pflanzengenetik, Frederik Boernke, Uwe Sonnewald filed Critical Ipk Inst Fuer Pflanzengenetik
Priority to EP01909747A priority Critical patent/EP1255841A2/de
Priority to AU2001237381A priority patent/AU2001237381A1/en
Publication of WO2001059131A2 publication Critical patent/WO2001059131A2/de
Publication of WO2001059131A3 publication Critical patent/WO2001059131A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/27—Erwinia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft rekombinante Nukleinsäuremoleküle, die eine DNA-Sequenz enthalten, die ein Protein mit der enzymatischen Aktivität einer Palatinose-Hydrolase, auch als Palatinase bezeichnet, bzw. einer Trehalulose-Hydrolase, auch als Trehalulase bezeichnet, kodiert, und die Verwendung dieser Nukleinsäuremoleküle als Selektionsmarker in transformierten Zellen. Insbesondere betrifft die Erfindung rekombinante Nukleinsäuremoleküle, die eine DNA-Sequenz enthalten, die ein Protein mit der enzymatischen Aktivität einer Palatinase bzw. einer Trehalulase kodiert, und worin diese DNA-Sequenz unter Kontrolle von regulatorischen Sequenzen eines in Pflanzen aktiven Promotors steht. Weiter betrifft die Erfindung Vektoren und Wirtszellen, die die erfindungsgemäßen rekombinanten Nukleinsäuremoleküle enthalten. Weiter werden Verfahren zur Herstellung transformierter Pflanzenzellen und Pflanzen unter Verwendung der rekombinanten Nukleinsäuremoleküle und Vektoren bereitgestellt. Weiter betrifft die Erfindung transgene Pflanzen, deren Ernteprodukte und Vermehrungsmaterial sowie transgene Pflanzenzellen, die die erfindungsgemäßen Nukleinsäuremoleküle oder Vektoren enthalten oder durch das erfindungsgemäßen Verfahren hergestellt werden. Die Erfindung betrifft insbesondere auch die Verwendung von DNA-Sequenze, die ein Protein mit der enzymatischen Aktivität einer Palatinose-Hydrolase bzw. einer Trehalulose-Hydrolase kodieren, als nutritiver Selektionsmarker in Pflanzenzellen zur Selektion transformierter Pflanzenzellen und Pflanzen. Des weiteren betrifft die Erfindung ein Verfahren zur Selektion von Mikroorganismen auf Palatinose- bzw. Trehalulose-haltigem Medium.
PCT/EP2001/001602 2000-02-14 2001-02-14 Verwendung von palatinase- und trehalulase-sequenzen als nutritive marker in transformierten zellen WO2001059131A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01909747A EP1255841A2 (de) 2000-02-14 2001-02-14 Verwendung von palatinase- und trehalulase-sequenzen als nutritive marker in transformierten zellen
AU2001237381A AU2001237381A1 (en) 2000-02-14 2001-02-14 Use of palatinase and trehalulase sequences as nutritive markers in transformed cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10006463 2000-02-14
DE10006463.9 2000-02-14
DE10045182A DE10045182A1 (de) 2000-02-14 2000-09-13 Verwendung von Palatinase- und Trehalulase-Sequenzen als nutritive Marker in transformierten Zellen
DE10045182.9 2000-09-13

Publications (2)

Publication Number Publication Date
WO2001059131A2 WO2001059131A2 (de) 2001-08-16
WO2001059131A3 true WO2001059131A3 (de) 2002-06-20

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ID=26004327

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Application Number Title Priority Date Filing Date
PCT/EP2001/001602 WO2001059131A2 (de) 2000-02-14 2001-02-14 Verwendung von palatinase- und trehalulase-sequenzen als nutritive marker in transformierten zellen

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EP (1) EP1255841A2 (de)
AU (1) AU2001237381A1 (de)
WO (1) WO2001059131A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102066567A (zh) 2008-06-11 2011-05-18 先正达参股股份有限公司 用于在植物中生产可发酵的碳水化合物的组合物和方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0567785A2 (de) * 1992-03-27 1993-11-03 Toyo Boseki Kabushiki Kaisha Protein mit Alpha-Glukosidase-Aktivität, DNS mit dessen genetischer Information und Herstellung von Alpha-Glukosidase
WO1995020047A2 (de) * 1994-01-19 1995-07-27 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Herstellung von akariogenen zuckerersatzstoffen
EP0751218A2 (de) * 1995-06-28 1997-01-02 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Saccharose-Stoffwechsel-Mutanten

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0567785A2 (de) * 1992-03-27 1993-11-03 Toyo Boseki Kabushiki Kaisha Protein mit Alpha-Glukosidase-Aktivität, DNS mit dessen genetischer Information und Herstellung von Alpha-Glukosidase
WO1995020047A2 (de) * 1994-01-19 1995-07-27 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Herstellung von akariogenen zuckerersatzstoffen
EP0751218A2 (de) * 1995-06-28 1997-01-02 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Saccharose-Stoffwechsel-Mutanten

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AU2001237381A1 (en) 2001-08-20
EP1255841A2 (de) 2002-11-13
WO2001059131A2 (de) 2001-08-16

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