WO2001059075A8 - Improved sialylation of glycoproteins - Google Patents

Improved sialylation of glycoproteins

Info

Publication number
WO2001059075A8
WO2001059075A8 PCT/US2001/003955 US0103955W WO0159075A8 WO 2001059075 A8 WO2001059075 A8 WO 2001059075A8 US 0103955 W US0103955 W US 0103955W WO 0159075 A8 WO0159075 A8 WO 0159075A8
Authority
WO
WIPO (PCT)
Prior art keywords
glycoproteins
epimerases
glcnac
udp
provides
Prior art date
Application number
PCT/US2001/003955
Other languages
French (fr)
Other versions
WO2001059075A9 (en
WO2001059075A1 (en
Inventor
Thomas Ryll
Original Assignee
Genentech Inc
Thomas Ryll
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc, Thomas Ryll filed Critical Genentech Inc
Priority to AU2001236739A priority Critical patent/AU2001236739A1/en
Publication of WO2001059075A1 publication Critical patent/WO2001059075A1/en
Publication of WO2001059075A8 publication Critical patent/WO2001059075A8/en
Publication of WO2001059075A9 publication Critical patent/WO2001059075A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides for cells and processes for producing glycoproteins having enhanced sialyation. The invention also provides for nucleic acid sequences encoding UDP-GlcNac 2-epimerases or modified UDP-GlcNac 2-epimerases. The modified UDP-GlcNac 2-epimerases have reduced binding to sialic acid.
PCT/US2001/003955 2000-02-08 2001-02-07 Improved sialylation of glycoproteins WO2001059075A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001236739A AU2001236739A1 (en) 2000-02-08 2001-02-07 Improved sialylation of glycoproteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18107400P 2000-02-08 2000-02-08
US60/181,074 2000-02-08
US19152900P 2000-03-23 2000-03-23
US60/191,529 2000-03-23

Publications (3)

Publication Number Publication Date
WO2001059075A1 WO2001059075A1 (en) 2001-08-16
WO2001059075A8 true WO2001059075A8 (en) 2002-03-07
WO2001059075A9 WO2001059075A9 (en) 2002-10-24

Family

ID=26876859

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/003955 WO2001059075A1 (en) 2000-02-08 2001-02-07 Improved sialylation of glycoproteins

Country Status (2)

Country Link
AU (1) AU2001236739A1 (en)
WO (1) WO2001059075A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003303394B2 (en) 2002-12-23 2009-02-19 Bristol-Myers Squibb Company Product quality enhancement in mammalian cell culture processes for protein production
ES2517603T3 (en) * 2008-04-07 2014-11-03 Bayer Healthcare, Llc Recombinant glycoprotein production procedures
US8940876B2 (en) 2010-02-08 2015-01-27 Korea Advanced Institute Of Science And Technology Method for preparing recombinant glycoproteins with high sialic acid content
US20130030040A1 (en) * 2011-02-01 2013-01-31 Hibm Research Group, Inc. Methods and compositions for increasing sialic acid production and treating sialic related disease conditions
GB201714765D0 (en) * 2017-09-14 2017-11-01 Vib Vzw Means and methods for the production of glycoproteins comprising homogeneous sialylated carbonhydrates
CN113337495B (en) * 2021-06-03 2022-10-11 江南大学 Method for improving sialic acid yield and application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3937891A1 (en) * 1989-11-15 1991-05-16 Forschungszentrum Juelich Gmbh ENZYMATIC PROCESS FOR THE PREPARATION OF N-ACETYLNEURAMINE ACID
US5721121A (en) * 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
AU3508300A (en) * 1999-03-02 2000-09-21 Human Genome Sciences, Inc. Engineering intracellular sialylation pathways

Also Published As

Publication number Publication date
AU2001236739A1 (en) 2001-08-20
WO2001059075A9 (en) 2002-10-24
WO2001059075A1 (en) 2001-08-16

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