WO2001051655A3 - Oligonukleotide zur amplifikation und zum nachweis von bakteriellen genen für extrazelluläre alkalische metallopeptidasen (apr), neutrale metallopeptidasen (npr) und serinpeptidasen (spr) - Google Patents

Oligonukleotide zur amplifikation und zum nachweis von bakteriellen genen für extrazelluläre alkalische metallopeptidasen (apr), neutrale metallopeptidasen (npr) und serinpeptidasen (spr) Download PDF

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Publication number
WO2001051655A3
WO2001051655A3 PCT/EP2001/000361 EP0100361W WO0151655A3 WO 2001051655 A3 WO2001051655 A3 WO 2001051655A3 EP 0100361 W EP0100361 W EP 0100361W WO 0151655 A3 WO0151655 A3 WO 0151655A3
Authority
WO
WIPO (PCT)
Prior art keywords
metalopeptidases
oligonucleotides
neutral
serin
npr
Prior art date
Application number
PCT/EP2001/000361
Other languages
English (en)
French (fr)
Other versions
WO2001051655A2 (de
Inventor
Hans-Juergen Bach
Original Assignee
Gsf Forschungszentrum Umwelt
Bach Hans Juergen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gsf Forschungszentrum Umwelt, Bach Hans Juergen filed Critical Gsf Forschungszentrum Umwelt
Priority to AU2001230183A priority Critical patent/AU2001230183A1/en
Priority to EP01902315A priority patent/EP1246943A2/de
Publication of WO2001051655A2 publication Critical patent/WO2001051655A2/de
Publication of WO2001051655A3 publication Critical patent/WO2001051655A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung beschreibt Oligonukleotide zum Nachweisbakterieller Gene, die für extrazelluläre alkalische Metalloproteasen, extrazelluläre neutrale Metalloproteasen und extrazelluläre Serinproteasen kodieren.
PCT/EP2001/000361 2000-01-13 2001-01-12 Oligonukleotide zur amplifikation und zum nachweis von bakteriellen genen für extrazelluläre alkalische metallopeptidasen (apr), neutrale metallopeptidasen (npr) und serinpeptidasen (spr) WO2001051655A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001230183A AU2001230183A1 (en) 2000-01-13 2001-01-12 Oligonucleotides for amplifying and detecting bacterial genes for extracellular alkaline metalopeptidases (apr), neutral metalopeptidases (npr) and serin peptidases (spr)
EP01902315A EP1246943A2 (de) 2000-01-13 2001-01-12 Oligonukleotide zur amplifikation und zum nachweis von bakteriellen genen für extrazelluläre alkalische metallopeptidasen (apr), neutrale metallopeptidasen (npr) und serinpeptidasen (spr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10001140.3 2000-01-13
DE10001140A DE10001140C2 (de) 2000-01-13 2000-01-13 Oligonukleotide zur Amplifikation und zum Nachweis von bakteriellen Genen für extrazelluläre alkalische Metallopeptidasen (Apr), neutrale Metallopeptidasen (Npr) und Serinpeptidasen (Spr)

Publications (2)

Publication Number Publication Date
WO2001051655A2 WO2001051655A2 (de) 2001-07-19
WO2001051655A3 true WO2001051655A3 (de) 2002-05-02

Family

ID=7627386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/000361 WO2001051655A2 (de) 2000-01-13 2001-01-12 Oligonukleotide zur amplifikation und zum nachweis von bakteriellen genen für extrazelluläre alkalische metallopeptidasen (apr), neutrale metallopeptidasen (npr) und serinpeptidasen (spr)

Country Status (4)

Country Link
EP (1) EP1246943A2 (de)
AU (1) AU2001230183A1 (de)
DE (1) DE10001140C2 (de)
WO (1) WO2001051655A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129096B2 (en) 2001-12-11 2006-10-31 Duke University Sensor for use in testing biological, biochemical, chemical or environmental samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007581A1 (en) * 1987-04-03 1988-10-06 Amgen Inc. Novel proteolytic enzymes
WO1996030526A2 (en) * 1995-03-31 1996-10-03 Ecogen Inc. Bacillus thuringiensis alkaline protease and neutral protease genes
EP0781853A2 (de) * 1995-12-12 1997-07-02 Eli Lilly And Company Verfahren zur Messung von Nukleinsäuren
US5756339A (en) * 1994-06-13 1998-05-26 Takara Shuzo Co., Ltd. Hyperthermostable protease gene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3025597A (en) * 1996-05-10 1997-12-05 Novo Nordisk A/S Method of providing novel dna sequences

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007581A1 (en) * 1987-04-03 1988-10-06 Amgen Inc. Novel proteolytic enzymes
US5756339A (en) * 1994-06-13 1998-05-26 Takara Shuzo Co., Ltd. Hyperthermostable protease gene
WO1996030526A2 (en) * 1995-03-31 1996-10-03 Ecogen Inc. Bacillus thuringiensis alkaline protease and neutral protease genes
EP0781853A2 (de) * 1995-12-12 1997-07-02 Eli Lilly And Company Verfahren zur Messung von Nukleinsäuren

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BACH H.-J. ET AL.: "PCR primers and functional probes for amplification and detection of bacterial genes for extracellular peptidases in single strains and in soil", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 44, March 2001 (2001-03-01), XP001061356 *
BACH, H.-J. ET AL.: "Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 7, July 1999 (1999-07-01), pages 3226 - 3228, XP001061359 *
JARAI G ET AL: "CLONING AND CHARACTERIZATION OF THE PEPD GENE OF ASPERGILLUS NIGER WHICH CODES FOR A SUBTILISIN-LIKE PROTEASE", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 139, no. 1, 1994, pages 51 - 57, XP001026238, ISSN: 0378-1119 *
MINE O M AND CARNEGIE P R: "Use of degenerate primers and heat-soaked polymerase chain reaction (PCR) to clone a serine protease antigen from Dermatophilus congolensis", IMMUNOLOGY AND CELL BIOLOGY, vol. 75, 1997, pages 484 - 491, XP008000691 *

Also Published As

Publication number Publication date
DE10001140A1 (de) 2001-07-26
EP1246943A2 (de) 2002-10-09
DE10001140C2 (de) 2003-06-12
WO2001051655A2 (de) 2001-07-19
AU2001230183A1 (en) 2001-07-24

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