WO2001046463A3 - Methodes de preparation et d'utilisation d'etalons internes destines a des dosages par amplification de l'acide nucleique - Google Patents

Methodes de preparation et d'utilisation d'etalons internes destines a des dosages par amplification de l'acide nucleique Download PDF

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Publication number
WO2001046463A3
WO2001046463A3 PCT/EP2000/012996 EP0012996W WO0146463A3 WO 2001046463 A3 WO2001046463 A3 WO 2001046463A3 EP 0012996 W EP0012996 W EP 0012996W WO 0146463 A3 WO0146463 A3 WO 0146463A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid amplification
amplification assays
preparation
methods
Prior art date
Application number
PCT/EP2000/012996
Other languages
English (en)
Other versions
WO2001046463A2 (fr
Inventor
Klaus Zimmermann
Peter Turecek
Hans-Peter Schwarz
Manfred Rieger
Original Assignee
Baxter Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Ag filed Critical Baxter Ag
Priority to AU31614/01A priority Critical patent/AU3161401A/en
Priority to EP00991227A priority patent/EP1244814A2/fr
Publication of WO2001046463A2 publication Critical patent/WO2001046463A2/fr
Publication of WO2001046463A3 publication Critical patent/WO2001046463A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Abstract

L'invention concerne des étalons internes de l'acide nucléique destinés à des dosages par amplification de l'acide nucléique. Plus particulièrement, l'invention concerne des étalons internes de l'acide nucléique préparés selon les techniques de l'ADN non recombiné. Ces étalons internes de l'acide nucléique sont globalement synthétisés par voie chimique et ont une taille minimale égale à environ 90 paires de bases d'acide nucléique. L'invention concerne également des étalons internes de l'acide nucléique préparés selon les techniques de l'ADN non recombiné, qui sont des acides nucléiques monocaténaires.
PCT/EP2000/012996 1999-12-22 2000-12-20 Methodes de preparation et d'utilisation d'etalons internes destines a des dosages par amplification de l'acide nucleique WO2001046463A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU31614/01A AU3161401A (en) 1999-12-22 2000-12-20 Methods for the preparation and use of internal standards for nucleic acid amplification assays
EP00991227A EP1244814A2 (fr) 1999-12-22 2000-12-20 Methodes de preparation et d'utilisation d'etalons internes destines a des dosages par amplification de l'acide nucleique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0217099A AT409383B (de) 1999-12-22 1999-12-22 Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
ATA2170/99 1999-12-22

Publications (2)

Publication Number Publication Date
WO2001046463A2 WO2001046463A2 (fr) 2001-06-28
WO2001046463A3 true WO2001046463A3 (fr) 2002-06-27

Family

ID=3529231

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012996 WO2001046463A2 (fr) 1999-12-22 2000-12-20 Methodes de preparation et d'utilisation d'etalons internes destines a des dosages par amplification de l'acide nucleique

Country Status (5)

Country Link
US (1) US20020102548A1 (fr)
EP (1) EP1244814A2 (fr)
AT (1) AT409383B (fr)
AU (1) AU3161401A (fr)
WO (1) WO2001046463A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1423534A4 (fr) * 2001-08-16 2006-08-30 Stratagene California Compositions et procedes comprenant un acide nucleique temoin
EP1457556A4 (fr) * 2001-12-28 2005-01-19 Takeda Pharmaceutical Procede et kit de quantification d'acides nucleiques
WO2004104229A2 (fr) * 2003-05-16 2004-12-02 Vickery Michael C L Molecule d'acide nucleique de controle interne pour systemes d'amplification d'acide nucleique
CA2502549C (fr) * 2004-04-23 2016-02-16 Becton, Dickinson And Company Utilisation d'un dispositif de controle de l'extraction au cours d'un procede d'extraction d'acides nucleiques
US20060110724A1 (en) 2004-11-19 2006-05-25 Burkhardt William Iii Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
EP2220227B1 (fr) * 2007-07-13 2014-09-10 The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense Polynucléotides calibrateurs uniques et procédés d'utilisation dans des dosages quantitatifs d'acide nucléique
KR20140006963A (ko) * 2011-02-25 2014-01-16 노파르티스 아게 외래 내부 양성 대조군
TW202336236A (zh) 2015-03-27 2023-09-16 美商再生元醫藥公司 偵測生物污染物之組成物及方法
WO2019161039A2 (fr) * 2018-02-14 2019-08-22 Regents Of The University Of Minnesota Étalons dimensionnel pour séquençage de nouvelle génération
RU2738798C1 (ru) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Способ количественного определения вирусного инфицирования сперматозоидов

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994004706A1 (fr) * 1992-08-24 1994-03-03 Akzo Nobel N.V. Elimination de faux negatifs en depistage d'acides nucleiques
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
WO1995002067A1 (fr) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Procede ameliore de quantification de l'acide nucleique
WO1999042618A1 (fr) * 1998-02-18 1999-08-26 Dade Behring Inc. Determination quantitative de produits d'amplification de l'acide nucleique

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994004706A1 (fr) * 1992-08-24 1994-03-03 Akzo Nobel N.V. Elimination de faux negatifs en depistage d'acides nucleiques
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
WO1995002067A1 (fr) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Procede ameliore de quantification de l'acide nucleique
WO1999042618A1 (fr) * 1998-02-18 1999-08-26 Dade Behring Inc. Determination quantitative de produits d'amplification de l'acide nucleique

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
FOELDES-PAPP ZENO ET AL: "Efficiency of chemical oligonucleotide synthesis evaluated by ion-exchange high-performance liquid chromatography.", JOURNAL OF CHROMATOGRAPHY A, vol. 706, no. 1-2, 1995, pages 405 - 419, XP004038891, ISSN: 0021-9673 *
ORRU DANIELA ET AL: "An improved protocol to generate high-efficiency single-stranded DNA probes by PCR.", BIOTECHNIQUES, vol. 14, no. 6, 1993, pages 905 - 906, XP002193313, ISSN: 0736-6205 *
ROSENSTRAUS MAURICE ET AL: "An internal control for routine diagnostic PCR: Design, properties, and effect of clinical performance.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 36, no. 1, January 1998 (1998-01-01), pages 191 - 197, XP002193312, ISSN: 0095-1137 *
SIEBERT P D ET AL: "PCR MIMICS: COMPETITIVE DNA FRAGMENTS FOR USE AS INTERNAL STANDARDS IN QUANTITATIVE PCR", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 14, no. 2, 1 March 1993 (1993-03-01), pages 244 - 246,248,24, XP000345591, ISSN: 0736-6205 *
TELENTI A ET AL: "Competitive polymerase chain reaction using an internal standard: application to the quantitation of viral DNA", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 39, no. 3, September 1992 (1992-09-01), pages 259 - 268, XP002115768, ISSN: 0166-0934 *
ZIMMERMANN K ET AL: "Sensitive single-stage PCR using custom-synthesized internal controls.", BIOTECHNIQUES, vol. 28, no. 4, April 2000 (2000-04-01), pages 694 - 702, XP002193315, ISSN: 0736-6205 *
ZIMMERMANN K ET AL: "TECHNICAL ASPECTS OF QUANTITATIVE COMPETITIVE PCR", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 21, no. 2, 1 August 1996 (1996-08-01), pages 268 - 270,272,27, XP000597689, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
AU3161401A (en) 2001-07-03
EP1244814A2 (fr) 2002-10-02
WO2001046463A2 (fr) 2001-06-28
AT409383B (de) 2002-07-25
ATA217099A (de) 2001-12-15
US20020102548A1 (en) 2002-08-01

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