WO2001046463A3 - Methods for the preparation and use of internal standards for nucleic acid amplification assays - Google Patents

Methods for the preparation and use of internal standards for nucleic acid amplification assays Download PDF

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Publication number
WO2001046463A3
WO2001046463A3 PCT/EP2000/012996 EP0012996W WO0146463A3 WO 2001046463 A3 WO2001046463 A3 WO 2001046463A3 EP 0012996 W EP0012996 W EP 0012996W WO 0146463 A3 WO0146463 A3 WO 0146463A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid amplification
amplification assays
preparation
methods
Prior art date
Application number
PCT/EP2000/012996
Other languages
French (fr)
Other versions
WO2001046463A2 (en
Inventor
Klaus Zimmermann
Peter Turecek
Hans-Peter Schwarz
Manfred Rieger
Original Assignee
Baxter Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Ag filed Critical Baxter Ag
Priority to AU31614/01A priority Critical patent/AU3161401A/en
Priority to EP00991227A priority patent/EP1244814A2/en
Publication of WO2001046463A2 publication Critical patent/WO2001046463A2/en
Publication of WO2001046463A3 publication Critical patent/WO2001046463A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Internal nucleic acid standards for nucleic acid amplification assays are provided. Specifically, internal nucleic acid standards are provided that are prepared using non-recombinant DNA technology. These internal nucleic acid standards are generally chemically synthesized and have a minimum size of approximately 90 nucleic acid bases. Also provided are internal nucleic acid standards prepared using non-recombinant DNA techniques that are single stranded nucleic acids.
PCT/EP2000/012996 1999-12-22 2000-12-20 Methods for the preparation and use of internal standards for nucleic acid amplification assays WO2001046463A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU31614/01A AU3161401A (en) 1999-12-22 2000-12-20 Methods for the preparation and use of internal standards for nucleic acid amplification assays
EP00991227A EP1244814A2 (en) 1999-12-22 2000-12-20 Methods for the preparation and use of internal standards for nucleic acid amplification assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA2170/99 1999-12-22
AT0217099A AT409383B (en) 1999-12-22 1999-12-22 METHOD FOR DETECTING AND QUANTIFYING NUCLEIC ACIDS IN A SAMPLE

Publications (2)

Publication Number Publication Date
WO2001046463A2 WO2001046463A2 (en) 2001-06-28
WO2001046463A3 true WO2001046463A3 (en) 2002-06-27

Family

ID=3529231

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012996 WO2001046463A2 (en) 1999-12-22 2000-12-20 Methods for the preparation and use of internal standards for nucleic acid amplification assays

Country Status (5)

Country Link
US (1) US20020102548A1 (en)
EP (1) EP1244814A2 (en)
AT (1) AT409383B (en)
AU (1) AU3161401A (en)
WO (1) WO2001046463A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1423534A4 (en) * 2001-08-16 2006-08-30 Stratagene California Compositions and methods comprising control nucleic acid
WO2003057880A1 (en) * 2001-12-28 2003-07-17 Takeda Chemical Industries, Ltd. Method of quantifying nucleic acid and kit for quantifying nucleic acid
EP1625232B1 (en) * 2003-05-16 2013-09-04 GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Internal control nucleic acid molecule for nucleic acid amplification systems
CA2502549C (en) 2004-04-23 2016-02-16 Becton, Dickinson And Company Use of an extraction control in a method of extracting nucleic acids
US20060110724A1 (en) 2004-11-19 2006-05-25 Burkhardt William Iii Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
EP2220227B1 (en) * 2007-07-13 2014-09-10 The United States of America, as represented by The Secretary of the Army, on behalf of the U.S. Army Med. Research Institute of Chem. Defense Unique calibrator polynucleotides and methods of using in quantitative nucleic acid assays
ES2572391T3 (en) * 2011-02-25 2016-05-31 Novartis Ag Positive internal exogenous control for virus detection
TW202336236A (en) * 2015-03-27 2023-09-16 美商再生元醫藥公司 Compositions and methods for detecting a biological contaminant
WO2019161039A2 (en) * 2018-02-14 2019-08-22 Regents Of The University Of Minnesota Size standards for next-generation sequencing
RU2738798C1 (en) * 2020-08-31 2020-12-16 Василий Васильевич Ашапкин Method for quantitative determination of viral infection of sperm cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994004706A1 (en) * 1992-08-24 1994-03-03 Akzo Nobel N.V. Elimination of false negatives in nucleic acid detection
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
WO1995002067A1 (en) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Improved method for the quantification of nucleic acid
WO1999042618A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Quantitative determination of nucleic acid amplification products

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994004706A1 (en) * 1992-08-24 1994-03-03 Akzo Nobel N.V. Elimination of false negatives in nucleic acid detection
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
WO1995002067A1 (en) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Improved method for the quantification of nucleic acid
WO1999042618A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Quantitative determination of nucleic acid amplification products

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
FOELDES-PAPP ZENO ET AL: "Efficiency of chemical oligonucleotide synthesis evaluated by ion-exchange high-performance liquid chromatography.", JOURNAL OF CHROMATOGRAPHY A, vol. 706, no. 1-2, 1995, pages 405 - 419, XP004038891, ISSN: 0021-9673 *
ORRU DANIELA ET AL: "An improved protocol to generate high-efficiency single-stranded DNA probes by PCR.", BIOTECHNIQUES, vol. 14, no. 6, 1993, pages 905 - 906, XP002193313, ISSN: 0736-6205 *
ROSENSTRAUS MAURICE ET AL: "An internal control for routine diagnostic PCR: Design, properties, and effect of clinical performance.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 36, no. 1, January 1998 (1998-01-01), pages 191 - 197, XP002193312, ISSN: 0095-1137 *
SIEBERT P D ET AL: "PCR MIMICS: COMPETITIVE DNA FRAGMENTS FOR USE AS INTERNAL STANDARDS IN QUANTITATIVE PCR", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 14, no. 2, 1 March 1993 (1993-03-01), pages 244 - 246,248,24, XP000345591, ISSN: 0736-6205 *
TELENTI A ET AL: "Competitive polymerase chain reaction using an internal standard: application to the quantitation of viral DNA", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 39, no. 3, September 1992 (1992-09-01), pages 259 - 268, XP002115768, ISSN: 0166-0934 *
ZIMMERMANN K ET AL: "Sensitive single-stage PCR using custom-synthesized internal controls.", BIOTECHNIQUES, vol. 28, no. 4, April 2000 (2000-04-01), pages 694 - 702, XP002193315, ISSN: 0736-6205 *
ZIMMERMANN K ET AL: "TECHNICAL ASPECTS OF QUANTITATIVE COMPETITIVE PCR", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 21, no. 2, 1 August 1996 (1996-08-01), pages 268 - 270,272,27, XP000597689, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
ATA217099A (en) 2001-12-15
EP1244814A2 (en) 2002-10-02
AU3161401A (en) 2001-07-03
US20020102548A1 (en) 2002-08-01
WO2001046463A2 (en) 2001-06-28
AT409383B (en) 2002-07-25

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