WO2001040507A3 - Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality - Google Patents

Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality Download PDF

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Publication number
WO2001040507A3
WO2001040507A3 PCT/AT2000/000324 AT0000324W WO0140507A3 WO 2001040507 A3 WO2001040507 A3 WO 2001040507A3 AT 0000324 W AT0000324 W AT 0000324W WO 0140507 A3 WO0140507 A3 WO 0140507A3
Authority
WO
WIPO (PCT)
Prior art keywords
chromosome
clonality
potentially
dns
methylated
Prior art date
Application number
PCT/AT2000/000324
Other languages
German (de)
French (fr)
Other versions
WO2001040507A2 (en
Inventor
Oskar A Haas
Andreas Weinhaeusel
Original Assignee
Forsch Krebskranke Kinder
Oskar A Haas
Andreas Weinhaeusel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forsch Krebskranke Kinder, Oskar A Haas, Andreas Weinhaeusel filed Critical Forsch Krebskranke Kinder
Priority to EP00984601A priority Critical patent/EP1238108A2/en
Priority to US10/148,570 priority patent/US20030170649A1/en
Priority to CA002388834A priority patent/CA2388834A1/en
Priority to AU21253/01A priority patent/AU2125301A/en
Publication of WO2001040507A2 publication Critical patent/WO2001040507A2/en
Priority to NO20022613A priority patent/NO20022613L/en
Publication of WO2001040507A3 publication Critical patent/WO2001040507A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for detecting and evaluating a potentially aberrantly methylated DNS region on the X chromosome or the clonality. The extend of the methylation of the potentially aberrantly methylated DNS region and optionally the extend of the methylation in a securely physiologically methylated DNS area of the X chromosome and/or the extend of the methylation as well as optionally the sequence variant of a polymorphous DNS region of the X-chromosome is/are determined in a sample, whereby said variant can be methylated physiologically or aberrantly. The presence and optionally the variant and the extend of the potential aberration or the clonality is/are subsequently diagnosed by comparing the determined methylations and optionally the sequence variant.
PCT/AT2000/000324 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality WO2001040507A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP00984601A EP1238108A2 (en) 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality
US10/148,570 US20030170649A1 (en) 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dna region on the x chromosome, or the clonality
CA002388834A CA2388834A1 (en) 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dna region on the x chromosome or the clonality
AU21253/01A AU2125301A (en) 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality
NO20022613A NO20022613L (en) 1999-12-03 2002-05-31 Method for detecting and evaluating a potentially aberrant methylated DNS region on the X chromosome or clonality

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA2041/99 1999-12-03
AT0204199A AT408989B (en) 1999-12-03 1999-12-03 METHOD FOR DETECTING AND EVALUATING A POTENTIALLY ABERRANTLY METHYLATED DNA REGION ON THE X-CHROMOSOME OR THE CLONALITY

Publications (2)

Publication Number Publication Date
WO2001040507A2 WO2001040507A2 (en) 2001-06-07
WO2001040507A3 true WO2001040507A3 (en) 2002-07-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2000/000324 WO2001040507A2 (en) 1999-12-03 2000-12-04 Method for detecting and evaluating a potentially aberrantly methylated dns region on the x chromosome or the clonality

Country Status (6)

Country Link
EP (1) EP1238108A2 (en)
AT (1) AT408989B (en)
AU (1) AU2125301A (en)
CA (1) CA2388834A1 (en)
NO (1) NO20022613L (en)
WO (1) WO2001040507A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107245519A (en) * 2017-06-06 2017-10-13 俞晓敏 A kind of PWS and AS quick determination method
CN108715869B (en) * 2018-06-01 2020-12-08 华南农业大学 Method for improving mammal cloning efficiency based on obtaining specific donor cells

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AMERICAN JOURNAL OF HUMAN GENETICS, vol. 65, no. 4, October 1999 (1999-10-01), 49th Annual Meeting of the American Society of Human Genetics;San Francisco, California, USA; October 19-23, 1999, pages A92, ISSN: 0002-9297 *
DAS S ET AL: "Methylation analysis of the fragile X syndrome by PCR.", GENETIC TESTING, vol. 1, no. 3, 1997, pages 151 - 155, XP001073407, ISSN: 1090-6576 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1999, PANAGOPOULOS IOANNIS ET AL: "A methylation PCR approach for detection of fragile X syndrome.", XP002195974, Database accession no. PREV199900369982 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; June 1999 (1999-06-01), PENA SERGIO D J ET AL: "Diagnosis of the fragile X syndrome in males using methylation-specific PCR of the FMR1 locus.", XP002195973, Database accession no. PREV199900384915 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; October 1999 (1999-10-01), HAAS O A ET AL: "A simple method for generating target sequences for methylation-sensitive PCR.", XP002195975, Database accession no. PREV199900497331 *
GENETICS AND MOLECULAR BIOLOGY, vol. 22, no. 2, June 1999 (1999-06-01), pages 169 - 172, ISSN: 1415-4757 *
HUMAN MUTATION, vol. 14, no. 1, 1999, pages 71 - 79, ISSN: 1059-7794 *
LEE SIEW-TEH ET AL: "Clonal determination by the fragile X (FMR1) and phosphoglycerate kinase (PGK) genes in hematological malignancies.", CANCER RESEARCH, vol. 54, no. 19, 1994, pages 5212 - 5216, XP002195970, ISSN: 0008-5472 *
TINKER ANNA V ET AL: "Induction of XIST expression from the human active X chromosome in mouse/human somatic cell hybrids by DNA demethylation.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 12, 15 June 1998 (1998-06-15), pages 2935 - 2940, XP002195972, ISSN: 0305-1048 *
WEINHAUSEL ANDREAS ET AL: "Evaluation of the fragile X (FRAXA) syndrome with methylation-sensitive PCR.", HUMAN GENETICS, vol. 108, no. 6, June 2001 (2001-06-01), pages 450 - 458, XP002195971, ISSN: 0340-6717 *

Also Published As

Publication number Publication date
NO20022613L (en) 2002-08-01
AT408989B (en) 2002-04-25
AU2125301A (en) 2001-06-12
NO20022613D0 (en) 2002-05-31
ATA204199A (en) 2001-09-15
WO2001040507A2 (en) 2001-06-07
EP1238108A2 (en) 2002-09-11
CA2388834A1 (en) 2001-06-07

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