WO2001038504A3 - Homologous recombination in plants - Google Patents

Homologous recombination in plants Download PDF

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Publication number
WO2001038504A3
WO2001038504A3 PCT/US2000/032289 US0032289W WO0138504A3 WO 2001038504 A3 WO2001038504 A3 WO 2001038504A3 US 0032289 W US0032289 W US 0032289W WO 0138504 A3 WO0138504 A3 WO 0138504A3
Authority
WO
WIPO (PCT)
Prior art keywords
vire2
reca
fusion proteins
homologous recombination
protein homologues
Prior art date
Application number
PCT/US2000/032289
Other languages
French (fr)
Other versions
WO2001038504A2 (en
Inventor
Michael Lassner
Steven Delcardayre
Original Assignee
Maxygen Inc
Michael Lassner
Steven Delcardayre
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Michael Lassner, Steven Delcardayre filed Critical Maxygen Inc
Priority to AU17998/01A priority Critical patent/AU1799801A/en
Publication of WO2001038504A2 publication Critical patent/WO2001038504A2/en
Publication of WO2001038504A3 publication Critical patent/WO2001038504A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for evolving recombinase protein homologues and RecA/VirE2 fusion proteins which complement VirE2 deficient Agrobacterium are provided. The use of recombinase protein homologues and RecA/VirE2 fusion proteins in the context of Agrobacterium mediated transformation are provided. Methods for producing transgenic organisms by homologous recombination using evolved recombinase proteins and Agrobacterium strains which express recombinase protein homologues or RecA/VirE2 fusion proteins are provided. Transgenic cells and organisms which have integrated an exogenous DNA sequence into a predetermined site in their genome are provided.
PCT/US2000/032289 1999-11-23 2000-11-22 Homologous recombination in plants WO2001038504A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU17998/01A AU1799801A (en) 1999-11-23 2000-11-22 Homologous recombination in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16745099P 1999-11-23 1999-11-23
US60/167,450 1999-11-23

Publications (2)

Publication Number Publication Date
WO2001038504A2 WO2001038504A2 (en) 2001-05-31
WO2001038504A3 true WO2001038504A3 (en) 2002-01-24

Family

ID=22607416

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032289 WO2001038504A2 (en) 1999-11-23 2000-11-22 Homologous recombination in plants

Country Status (2)

Country Link
AU (1) AU1799801A (en)
WO (1) WO2001038504A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1015252C2 (en) * 2000-05-19 2001-11-20 Univ Leiden Method for effecting a change in a cell, and a vector.
EP2166099B1 (en) 2005-03-08 2012-12-19 BASF Plant Science GmbH Expression enhancing intron sequences
JP5944320B2 (en) 2009-11-27 2016-07-05 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー Optimized endonuclease and its use
CN105384827A (en) 2009-11-27 2016-03-09 巴斯夫植物科学有限公司 Chimeric endonucleases and uses thereof
JP2013511979A (en) 2009-11-27 2013-04-11 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー Chimeric endonuclease and use thereof
CN106459884A (en) * 2014-05-16 2017-02-22 株式会社钟化 Agrobacterium and transgenic plant manufacturing method using such agrobacterium
CN106106161B (en) * 2016-06-29 2018-08-17 云南纳博生物科技有限公司 A method of establishing agriculture bacillus mediated wide leaf the secular bird rotaring redyeing system
CN115346608B (en) * 2022-06-27 2023-05-09 北京吉因加科技有限公司 Method and device for constructing pathogenic organism genome database

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
WO1997008331A1 (en) * 1995-08-30 1997-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Stimulation of homologous recombination in eukaryotic organisms or cells by recombination promoting enzymes
WO1998031837A1 (en) * 1997-01-17 1998-07-23 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
WO1997008331A1 (en) * 1995-08-30 1997-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Stimulation of homologous recombination in eukaryotic organisms or cells by recombination promoting enzymes
WO1998031837A1 (en) * 1997-01-17 1998-07-23 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRENDEL VOLKER ET AL: "Evolutionary comparisons of RecA-like proteins across all major kingdoms of living organisms.", JOURNAL OF MOLECULAR EVOLUTION, vol. 44, no. 5, 1997, pages 528 - 541, XP001001339, ISSN: 0022-2844 *
CITOVSKY V ET AL: "NUCLEAR LOCALIZATION OF AGROBACTERIUM VIRE2 PROTEIN IN PLANT CELLS", SCIENCE (WASHINGTON D C), vol. 256, no. 5065, 1992, pages 1802 - 1805, XP002024324, ISSN: 0036-8075 *
OGAWA T ET AL: "FUNCTIONAL STRUCTURES OF THE RECA PROTEIN FOUND BY CHIMERA ANALYSIS", JOURNAL OF MOLECULAR BIOLOGY, vol. 226, no. 3, 1992, pages 651 - 660, XP001001237, ISSN: 0022-2836 *
SHCHERBAKOVA OLGA G ET AL: "Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells.", MUTATION RESEARCH., vol. 459, no. 1, 16 February 2000 (2000-02-16), pages 65 - 71, XP001004532, ISSN: 0027-5107 *
ZIEMIENOWICZ A ET AL: "Import of agrobacterium T-DNA into plant nuclei: Two distinct functions of VirD2 and VirE2 proteins", PLANT CELL, vol. 13, no. 2, February 2001 (2001-02-01), pages 369 - 383, XP001001554 *

Also Published As

Publication number Publication date
WO2001038504A2 (en) 2001-05-31
AU1799801A (en) 2001-06-04

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