WO2001036671A8 - A new method for the examination of protein localization in living cells - Google Patents

A new method for the examination of protein localization in living cells

Info

Publication number
WO2001036671A8
WO2001036671A8 PCT/EP2000/010943 EP0010943W WO0136671A8 WO 2001036671 A8 WO2001036671 A8 WO 2001036671A8 EP 0010943 W EP0010943 W EP 0010943W WO 0136671 A8 WO0136671 A8 WO 0136671A8
Authority
WO
WIPO (PCT)
Prior art keywords
examination
cell
new method
living cells
protein localization
Prior art date
Application number
PCT/EP2000/010943
Other languages
French (fr)
Other versions
WO2001036671A2 (en
WO2001036671A3 (en
Inventor
Fabian Koehler
Original Assignee
Fabian Koehler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fabian Koehler filed Critical Fabian Koehler
Priority to AU23545/01A priority Critical patent/AU2354501A/en
Publication of WO2001036671A2 publication Critical patent/WO2001036671A2/en
Publication of WO2001036671A8 publication Critical patent/WO2001036671A8/en
Publication of WO2001036671A3 publication Critical patent/WO2001036671A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method for the detection of a compound within a cell comprising the steps of (a) introducing into a cell proteinaceous material comprising at least two fusion proteins or derivatives thereof or nucleic acid, encoding upon expression said fusion proteins, wherein said compound or a precursor thereof is present or expressed in said cell before said proteinaceous material is introduced into or expressed in said cell; (b) allowing expression of said nucleic acid, if applicable; and (c) assessing for a signal from said first or second signaling entity that is provided, restored, altered or influenced.
PCT/EP2000/010943 1999-11-04 2000-11-06 A new method for the examination of protein localization in living cells WO2001036671A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU23545/01A AU2354501A (en) 1999-11-04 2000-11-06 A new method for the examination of protein localization in living cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99121887.6 1999-11-04
EP99121887 1999-11-04

Publications (3)

Publication Number Publication Date
WO2001036671A2 WO2001036671A2 (en) 2001-05-25
WO2001036671A8 true WO2001036671A8 (en) 2001-10-18
WO2001036671A3 WO2001036671A3 (en) 2004-04-01

Family

ID=8239337

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010943 WO2001036671A2 (en) 1999-11-04 2000-11-06 A new method for the examination of protein localization in living cells

Country Status (2)

Country Link
AU (1) AU2354501A (en)
WO (1) WO2001036671A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2078750T3 (en) * 2008-01-09 2017-01-02 Univ Muenchen Ludwig Maximilians Fluorescent two-hybrid (F2H) assay for direct visualization of protein interactions in living cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6702398A (en) * 1997-03-14 1998-09-29 Regents Of The University Of California, The Fluorescent protein sensors for detection of analytes
DE19829495A1 (en) * 1998-07-02 2000-01-05 Jacques Paysan Reagents and their application for the investigation of interactions between cellular molecules and their localization in cells

Also Published As

Publication number Publication date
WO2001036671A2 (en) 2001-05-25
AU2354501A (en) 2001-05-30
WO2001036671A3 (en) 2004-04-01

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