WO2001036671A3 - A new method for the examination of protein localization in living cells - Google Patents
A new method for the examination of protein localization in living cells Download PDFInfo
- Publication number
- WO2001036671A3 WO2001036671A3 PCT/EP2000/010943 EP0010943W WO0136671A3 WO 2001036671 A3 WO2001036671 A3 WO 2001036671A3 EP 0010943 W EP0010943 W EP 0010943W WO 0136671 A3 WO0136671 A3 WO 0136671A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- examination
- cell
- new method
- living cells
- protein localization
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU23545/01A AU2354501A (en) | 1999-11-04 | 2000-11-06 | A new method for the examination of protein localization in living cells |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP99121887.6 | 1999-11-04 | ||
EP99121887 | 1999-11-04 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2001036671A2 WO2001036671A2 (en) | 2001-05-25 |
WO2001036671A8 WO2001036671A8 (en) | 2001-10-18 |
WO2001036671A3 true WO2001036671A3 (en) | 2004-04-01 |
Family
ID=8239337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2000/010943 WO2001036671A2 (en) | 1999-11-04 | 2000-11-06 | A new method for the examination of protein localization in living cells |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2354501A (en) |
WO (1) | WO2001036671A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PL2078750T3 (en) * | 2008-01-09 | 2017-06-30 | Ludwig-Maximilians-Universität München | A fluorescent two-hybrid (F2H) assay for direct visualization of protein interactions in living cells |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998040477A1 (en) * | 1997-03-14 | 1998-09-17 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
EP0969284A1 (en) * | 1998-07-02 | 2000-01-05 | Jacques Paysan | Investigation of interaction between cellular molecules and their localisation in cells |
-
2000
- 2000-11-06 WO PCT/EP2000/010943 patent/WO2001036671A2/en active Application Filing
- 2000-11-06 AU AU23545/01A patent/AU2354501A/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998040477A1 (en) * | 1997-03-14 | 1998-09-17 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
EP0969284A1 (en) * | 1998-07-02 | 2000-01-05 | Jacques Paysan | Investigation of interaction between cellular molecules and their localisation in cells |
Non-Patent Citations (5)
Title |
---|
BIONDI RICHARD O ET AL: "Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 21, 1 November 1998 (1998-11-01), pages 4946 - 4952, XP002187520, ISSN: 0305-1048 * |
EMMANOUILIDOU EVAGGELIA ET AL: "Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon.", CURRENT BIOLOGY, vol. 9, no. 16, 26 August 1999 (1999-08-26), pages 915 - 918, XP001052939, ISSN: 0960-9822 * |
MAHAJAN N P ET AL: "BCL-2 AND BAX INTERACTIONS IN MITOCHONDRIA PROBED WITH GREEN FLUORESCENT PROTEIN AND FLUORESCENCE RESONANCE ENERGY TRANSFER", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, June 1998 (1998-06-01), pages 547 - 552, XP000876894, ISSN: 1087-0156 * |
MIYAWAKI ATSUSHI ET AL: "Fluorescent indicators for Ca-2+ based on green fluorescent proteins and calmodulin.", NATURE (LONDON), vol. 388, no. 6645, 1997, pages 882 - 887, XP002187517, ISSN: 0028-0836 * |
XU YAO ET AL: "A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 1, 5 January 1999 (1999-01-05), Jan. 5, 1999, pages 151 - 156, XP002187519, ISSN: 0027-8424 * |
Also Published As
Publication number | Publication date |
---|---|
WO2001036671A2 (en) | 2001-05-25 |
AU2354501A (en) | 2001-05-30 |
WO2001036671A8 (en) | 2001-10-18 |
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