WO2001034780A3 - Cytochrome p450 oxygenases and their uses - Google Patents

Cytochrome p450 oxygenases and their uses Download PDF

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Publication number
WO2001034780A3
WO2001034780A3 PCT/US2000/031254 US0031254W WO0134780A3 WO 2001034780 A3 WO2001034780 A3 WO 2001034780A3 US 0031254 W US0031254 W US 0031254W WO 0134780 A3 WO0134780 A3 WO 0134780A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
oxygenases
cytochrome
disclosed
oxygenase
Prior art date
Application number
PCT/US2000/031254
Other languages
French (fr)
Other versions
WO2001034780A2 (en
Inventor
Rodney B Croteau
Anne Schoendorf
Stefan Jennewein
Original Assignee
Univ Washington
Rodney B Croteau
Anne Schoendorf
Stefan Jennewein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington, Rodney B Croteau, Anne Schoendorf, Stefan Jennewein filed Critical Univ Washington
Priority to MXPA02004785A priority Critical patent/MXPA02004785A/en
Priority to CA002390504A priority patent/CA2390504A1/en
Priority to AU14887/01A priority patent/AU780594B2/en
Priority to EP00977218A priority patent/EP1232268A2/en
Priority to JP2001537477A priority patent/JP2003513665A/en
Publication of WO2001034780A2 publication Critical patent/WO2001034780A2/en
Publication of WO2001034780A3 publication Critical patent/WO2001034780A3/en
Priority to US10/142,231 priority patent/US6787343B2/en
Priority to US10/356,153 priority patent/US20030166176A1/en
Priority to US10/884,115 priority patent/US7005283B2/en
Priority to US11/242,662 priority patent/US7416872B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Epoxy Compounds (AREA)

Abstract

Oxygenase enzymes and the use of such enzymes to produce paclitaxel (TaxolTM), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
PCT/US2000/031254 1999-11-12 2000-11-13 Cytochrome p450 oxygenases and their uses WO2001034780A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
MXPA02004785A MXPA02004785A (en) 1999-11-12 2000-11-13 Cytochrome p450 oxygenases and their uses.
CA002390504A CA2390504A1 (en) 1999-11-12 2000-11-13 Cytochrome p450 oxygenases and their uses
AU14887/01A AU780594B2 (en) 1999-11-12 2000-11-13 Cytochrome P450 oxygenases and their uses
EP00977218A EP1232268A2 (en) 1999-11-12 2000-11-13 Cytochrome p450 oxygenases and their uses
JP2001537477A JP2003513665A (en) 1999-11-12 2000-11-13 Cytochrome P450 oxygenases and uses thereof
US10/142,231 US6787343B2 (en) 1999-11-12 2002-05-08 Cytochrome P450 oxygenases and their uses
US10/356,153 US20030166176A1 (en) 1999-11-12 2003-01-29 Taxoid-14-beta-hydroxylases and methods for their use
US10/884,115 US7005283B2 (en) 1999-11-12 2004-07-01 Cytochrome P450 oxygenases and their uses
US11/242,662 US7416872B2 (en) 1999-11-12 2005-10-03 Taxoid oxygenases and their uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16525099P 1999-11-12 1999-11-12
US60/165,250 1999-11-12

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/142,231 Continuation US6787343B2 (en) 1999-11-12 2002-05-08 Cytochrome P450 oxygenases and their uses

Publications (2)

Publication Number Publication Date
WO2001034780A2 WO2001034780A2 (en) 2001-05-17
WO2001034780A3 true WO2001034780A3 (en) 2001-11-08

Family

ID=22598102

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031254 WO2001034780A2 (en) 1999-11-12 2000-11-13 Cytochrome p450 oxygenases and their uses

Country Status (6)

Country Link
EP (1) EP1232268A2 (en)
JP (1) JP2003513665A (en)
AU (1) AU780594B2 (en)
CA (1) CA2390504A1 (en)
MX (1) MXPA02004785A (en)
WO (1) WO2001034780A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004035745A2 (en) * 2002-10-16 2004-04-29 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
EP1649005A2 (en) * 2003-07-22 2006-04-26 Washington State University Research Foundation P450 oxygenases and methods of use
CN112424338A (en) * 2018-07-17 2021-02-26 科纳根公司 Biosynthetic production of gamma-lactones

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040470A2 (en) * 1997-03-07 1998-09-17 Novartis Ag Cytochrome p450 monooxygenases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040470A2 (en) * 1997-03-07 1998-09-17 Novartis Ag Cytochrome p450 monooxygenases

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CABELLO-HURTADO ET AL.: "Cloning, expression in yeast, and functional characterization of CYP81B1, a plant cytochrome P450 that catalyzes in-chain hydroxylation of fatty acids", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 13, 27 March 1998 (1998-03-27), pages 7260 - 7267, XP002165911 *
EISENREICH ET AL.: "Multiple oxygenase reactions in the biosynthesis of taxoids", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 120, no. 37, 23 September 1998 (1998-09-23), pages 9694 - 9695, XP002165912 *
HEFNER ET AL.: "Cytochrome P450-catalyzed hydroxylation of taxa-4(5),11(12)-diene to taxa-4(20),11(12)-dien-5alpha-ol: the first oxygenation step in taxol biosynthesis", CHEMISTRY & BIOLOGY, vol. 3, June 1996 (1996-06-01), pages 479 - 489, XP000997470 *
PAULI ET AL.: "Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis", THE PLANT JOURNAL, vol. 13, no. 6, 1998, pages 793 - 801, XP000992757 *
SCHOENDORF ET AL.: "Molecular cloning of a cytochrome P450 taxane 10beta-hydroxylase cDNA from Taxus and functional expression in yeast", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE USA, vol. 98, no. 4, 13 February 2001 (2001-02-13), pages 1501 - 1506, XP002165913 *
TSUMURA ET AL.: "Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers", THEOR APPL GENET, vol. 94, 1997, pages 764 - 772, XP000998169 *
WILDUNG M R ET AL: "CDNA CLONE FOR TAXADIENE SYNTHASE, THE DITERPENE CYCLASE THAT CATALYZES THE COMMITTED STEP OF TAXOL BIOSYNTHESIS", JOURNAL OF BIOLOGICAL CHEMISTRY,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD,US, vol. 271, no. 16, 19 April 1996 (1996-04-19), pages 9201 - 9204, XP000938117, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
JP2003513665A (en) 2003-04-15
AU1488701A (en) 2001-06-06
EP1232268A2 (en) 2002-08-21
MXPA02004785A (en) 2003-10-14
AU780594B2 (en) 2005-04-07
WO2001034780A2 (en) 2001-05-17
CA2390504A1 (en) 2001-05-17

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