WO2001032911A3 - Toxicity assay using pva-immobilised luminescent bacteria - Google Patents

Toxicity assay using pva-immobilised luminescent bacteria Download PDF

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Publication number
WO2001032911A3
WO2001032911A3 PCT/GB2000/004182 GB0004182W WO0132911A3 WO 2001032911 A3 WO2001032911 A3 WO 2001032911A3 GB 0004182 W GB0004182 W GB 0004182W WO 0132911 A3 WO0132911 A3 WO 0132911A3
Authority
WO
WIPO (PCT)
Prior art keywords
immobilised
bacteria
pva
toxicity assay
luminescent bacteria
Prior art date
Application number
PCT/GB2000/004182
Other languages
French (fr)
Other versions
WO2001032911A2 (en
Inventor
James Crawford Philp
Original Assignee
Univ Napier
James Crawford Philp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Napier, James Crawford Philp filed Critical Univ Napier
Priority to AU11551/01A priority Critical patent/AU1155101A/en
Priority to GB0212839A priority patent/GB2373513B/en
Publication of WO2001032911A2 publication Critical patent/WO2001032911A2/en
Publication of WO2001032911A3 publication Critical patent/WO2001032911A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2520/00—Use of whole organisms as detectors of pollution

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides an improved bacterial cell biosensor. More specifically, an immobilisation system for a bioluminescent bacteria is provided, wherein natural and genetically modified bacteria for use in toxicity testing are immobilised in a polyvinyl alcohol thin film, this thin film allowing operation with an appropriate instrument. The immobilisation of the bacteria in the film results in the retention of the luminescent properties such that they can be used for the qualitative and quantitative detection of pollutant chemicals and industrial effluence in the environment.
PCT/GB2000/004182 1999-11-01 2000-11-01 Toxicity assay using pva-immobilised luminescent bacteria WO2001032911A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU11551/01A AU1155101A (en) 1999-11-01 2000-11-01 Immobilised bacteria
GB0212839A GB2373513B (en) 1999-11-01 2000-11-01 Biosensor comprising a bioluminescent bacteria immobilised in a PVA film

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9925788.3 1999-11-01
GBGB9925788.3A GB9925788D0 (en) 1999-11-01 1999-11-01 Immobilised bacteria

Publications (2)

Publication Number Publication Date
WO2001032911A2 WO2001032911A2 (en) 2001-05-10
WO2001032911A3 true WO2001032911A3 (en) 2002-07-11

Family

ID=10863717

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004182 WO2001032911A2 (en) 1999-11-01 2000-11-01 Toxicity assay using pva-immobilised luminescent bacteria

Country Status (3)

Country Link
AU (1) AU1155101A (en)
GB (2) GB9925788D0 (en)
WO (1) WO2001032911A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1309720B1 (en) * 2000-07-11 2009-09-16 Chesapeake Techlabs, Inc. Apparatus and method for detecting and classifying chemicals, particles, viruses, and bacteria in fluids with an aptamer
DE10219031A1 (en) * 2002-04-29 2003-11-20 Bayer Ag Detecting biologically active compounds, useful for detecting toxins and in drug screening, based on their effect on luminescent bacteria
EP1359226B1 (en) 2002-04-29 2006-11-22 Bayer Innovation GmbH Method and device for detection of biologically active substances
DE102007003577A1 (en) 2007-01-24 2008-08-07 Julius-Maximilians-Universität Würzburg Two-component system for programming bacterial membranes
US8389263B2 (en) 2007-09-10 2013-03-05 Sylvia Daunert Spores for the stabilization and on-site application of bacterial whole-cell biosensing systems

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62275683A (en) * 1986-05-26 1987-11-30 Agency Of Ind Science & Technol Thin film of immobilized enzyme
WO1999027351A1 (en) * 1997-11-25 1999-06-03 Lockheed Martin Energy Research Corporation Bioluminescent bioreporter integrated circuit

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62275683A (en) * 1986-05-26 1987-11-30 Agency Of Ind Science & Technol Thin film of immobilized enzyme
WO1999027351A1 (en) * 1997-11-25 1999-06-03 Lockheed Martin Energy Research Corporation Bioluminescent bioreporter integrated circuit

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARIGA OSAMU ET AL: "Encapsulation of biocatalyst with PVA capsules.", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 78, no. 1, 1994, pages 74 - 78, XP000998749, ISSN: 0922-338X *
LOZINSKY V I ET AL: "POLY(VINYL ALCOHOL) CRYOGELS EMPLOYED AS MATRICES FOR CELL IMMOBILIZATION. 3. OVERVIEW OF RECENT RESEARCH AND DEVELOPMENTS", ENZYME AND MICROBIAL TECHNOLOGY,STONEHAM, MA,US, vol. 23, no. 3/04, 15 September 1998 (1998-09-15), pages 227 - 242, XP000931186, ISSN: 0141-0229 *
O F WEBB ET AL: "Kinetics and response of Pseudomonas fluorescens HK44 biosensor", BIOTECHNOLOGY AND BIOENGINEERING,GB,INTERSCIENCE PUBLISHERS, LONDON, vol. 54, no. 5, 5 June 1997 (1997-06-05), pages 491 - 502, XP002098469, ISSN: 0006-3592 *
PATENT ABSTRACTS OF JAPAN vol. 012, no. 165 (C - 496) 18 May 1988 (1988-05-18) *
ROMANOVA N A ET AL: "Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant Escherichia coli cells expressing firefly luciferase.", BIOCHEMISTRY (MOSCOW), vol. 63, no. 5, May 1998 (1998-05-01), pages 579 - 583, XP000998782, ISSN: 0006-2979 *

Also Published As

Publication number Publication date
AU1155101A (en) 2001-05-14
WO2001032911A2 (en) 2001-05-10
GB9925788D0 (en) 1999-12-29
GB2373513B (en) 2004-08-25
GB0212839D0 (en) 2002-07-17
GB2373513A (en) 2002-09-25

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