GB2373513A - Immobilised bacteria - Google Patents

Immobilised bacteria Download PDF

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Publication number
GB2373513A
GB2373513A GB0212839A GB0212839A GB2373513A GB 2373513 A GB2373513 A GB 2373513A GB 0212839 A GB0212839 A GB 0212839A GB 0212839 A GB0212839 A GB 0212839A GB 2373513 A GB2373513 A GB 2373513A
Authority
GB
United Kingdom
Prior art keywords
bacteria
thin film
immobilised
immobilisation
qualitative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0212839A
Other versions
GB2373513B (en
GB0212839D0 (en
Inventor
James Crawford Philip
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Edinburgh Napier University
Original Assignee
Edinburgh Napier University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Edinburgh Napier University filed Critical Edinburgh Napier University
Publication of GB0212839D0 publication Critical patent/GB0212839D0/en
Publication of GB2373513A publication Critical patent/GB2373513A/en
Application granted granted Critical
Publication of GB2373513B publication Critical patent/GB2373513B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2520/00—Use of whole organisms as detectors of pollution

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides an improved bacterial cell biosensor. More specifically, an immobilisation system for a bioluminescent bacteria is provided, wherein natural and genetically modified bacteria for use in toxicity testing are immobilised in a polyvinyl alcohol thin film, this thin film allowing operation with an appropriate instrument. The immobilisation of the bacteria in the film results in the retention of the luminescent properties such that they can be used for the qualitative and quantitative detection of pollutant chemicals and industrial effluence in the environment.

Description

AUK Patent Application,g,GB ',2 373 513. ,A (43} Date of Printing by UK
Office 25.09.2002 (21) Application No 0212839.5 (51) INTCL7 C12Q 1/02, C12N 15/11, C12Q 1/66 /l ( C12Q 1/02 (22) Date of Filing 01.11.2000 C12R 1:185 1:40 1:63) (30) Priority Data (52) UK CL (Edition T) (31) 9925788 (32) 01.11.1999 (33) GB C6F FE F101
C3H HB7E
(86) International Application Data COY YG03 Y153 Y186 (56) Documents Cited by ISA (87) International Publication Data NOT YET ADVISED (58) Field of Search by ISA
NOT YET ADVISED
(71) Applicant(s) The Court of Napier University (Incorporated in the United Kingdom) (74) Agent and/or Address for Service Napier University Ventures Limited, Murgitroyd & Company Merchiston Tower, 10 Colinton Road, EDINBURGH, Scotland House, 165-169 Scotland Street, EH10 5DT, United Kingdom GLASGOW, G5 8PL United Kingdom (72) Inventor(s) James Crawford Philip (54} Abstract Title Immobilised bacteria (57} The present invention provides an improved bacterial cell biosensor. More specifically, an immobilization system for a bioluminescent bacteria is provided, wherein natural and genetically modified bacteria for use in toxicity testing are immobilised in a polyvinyl alcohol thin film, this thin film allowing operation with an appropriate instrument. The immobilization of the bacteria in the film results in the retention of the luminescent properties such that they can be used for the qualitative and quantitative detection of pollutant chemicals and industrial effluence in the environment.
C) W Cal
GB0212839A 1999-11-01 2000-11-01 Biosensor comprising a bioluminescent bacteria immobilised in a PVA film Expired - Fee Related GB2373513B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9925788.3A GB9925788D0 (en) 1999-11-01 1999-11-01 Immobilised bacteria
PCT/GB2000/004182 WO2001032911A2 (en) 1999-11-01 2000-11-01 Toxicity assay using pva-immobilised luminescent bacteria

Publications (3)

Publication Number Publication Date
GB0212839D0 GB0212839D0 (en) 2002-07-17
GB2373513A true GB2373513A (en) 2002-09-25
GB2373513B GB2373513B (en) 2004-08-25

Family

ID=10863717

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB9925788.3A Ceased GB9925788D0 (en) 1999-11-01 1999-11-01 Immobilised bacteria
GB0212839A Expired - Fee Related GB2373513B (en) 1999-11-01 2000-11-01 Biosensor comprising a bioluminescent bacteria immobilised in a PVA film

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB9925788.3A Ceased GB9925788D0 (en) 1999-11-01 1999-11-01 Immobilised bacteria

Country Status (3)

Country Link
AU (1) AU1155101A (en)
GB (2) GB9925788D0 (en)
WO (1) WO2001032911A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007003577A1 (en) 2007-01-24 2008-08-07 Julius-Maximilians-Universität Würzburg Two-component system for programming bacterial membranes

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1309720B1 (en) 2000-07-11 2009-09-16 Chesapeake Techlabs, Inc. Apparatus and method for detecting and classifying chemicals, particles, viruses, and bacteria in fluids with an aptamer
EP1359226B1 (en) 2002-04-29 2006-11-22 Bayer Innovation GmbH Method and device for detection of biologically active substances
DE10219031A1 (en) * 2002-04-29 2003-11-20 Bayer Ag Detecting biologically active compounds, useful for detecting toxins and in drug screening, based on their effect on luminescent bacteria
WO2009036070A1 (en) 2007-09-10 2009-03-19 University Of Kentucky Research Foundation Spores for the stabilization and on-site application of bacterial whole-cell biosensing systems

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62275683A (en) * 1986-05-26 1987-11-30 Agency Of Ind Science & Technol Thin film of immobilized enzyme
US6117643A (en) * 1997-11-25 2000-09-12 Ut Battelle, Llc Bioluminescent bioreporter integrated circuit

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NOT YET ADVISED *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007003577A1 (en) 2007-01-24 2008-08-07 Julius-Maximilians-Universität Würzburg Two-component system for programming bacterial membranes

Also Published As

Publication number Publication date
GB2373513B (en) 2004-08-25
GB9925788D0 (en) 1999-12-29
WO2001032911A2 (en) 2001-05-10
WO2001032911A3 (en) 2002-07-11
AU1155101A (en) 2001-05-14
GB0212839D0 (en) 2002-07-17

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20101101