WO2001030970A3 - Improved protocol for activation of oocytes - Google Patents

Improved protocol for activation of oocytes Download PDF

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Publication number
WO2001030970A3
WO2001030970A3 PCT/US2000/029552 US0029552W WO0130970A3 WO 2001030970 A3 WO2001030970 A3 WO 2001030970A3 US 0029552 W US0029552 W US 0029552W WO 0130970 A3 WO0130970 A3 WO 0130970A3
Authority
WO
WIPO (PCT)
Prior art keywords
activation
oocytes
olomoucine
improved protocol
protocol
Prior art date
Application number
PCT/US2000/029552
Other languages
French (fr)
Other versions
WO2001030970A2 (en
Inventor
Jose Cibelli
Original Assignee
Advanced Cell Tech Inc
Jose Cibelli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Tech Inc, Jose Cibelli filed Critical Advanced Cell Tech Inc
Priority to AU12354/01A priority Critical patent/AU1235401A/en
Publication of WO2001030970A2 publication Critical patent/WO2001030970A2/en
Publication of WO2001030970A3 publication Critical patent/WO2001030970A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/405—Cell cycle regulated proteins, e.g. cyclins, cyclin-dependant kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Pyrane Compounds (AREA)

Abstract

A novel protocol for activation of mammalian oocytes or nuclear transfer fusions that use a CDC2 or CDK2 specific inhibitor selected from roscovitine, iso-olomoucine, olomoucine, flavopiridol, and DMAP is provided. This protocol provides for more efficient formation of blastocysts which can be used to produce cloned offspring and embryonic stem cells.
PCT/US2000/029552 1999-10-27 2000-10-27 Improved protocol for activation of oocytes WO2001030970A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU12354/01A AU1235401A (en) 1999-10-27 2000-10-27 Improved protocol for activation of oocytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16173999P 1999-10-27 1999-10-27
US60/161,739 1999-10-27

Publications (2)

Publication Number Publication Date
WO2001030970A2 WO2001030970A2 (en) 2001-05-03
WO2001030970A3 true WO2001030970A3 (en) 2001-10-04

Family

ID=22582509

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/029552 WO2001030970A2 (en) 1999-10-27 2000-10-27 Improved protocol for activation of oocytes

Country Status (2)

Country Link
AU (1) AU1235401A (en)
WO (1) WO2001030970A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001090185A2 (en) 2000-05-25 2001-11-29 Queen's University At Kingston Pt32 sperm protein, sperm c-yes, oocyte cytoplasmic c-yes, and uses thereof
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
US20030232430A1 (en) * 2001-11-26 2003-12-18 Advanced Cell Technology Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ABRAHAM R.T. ET AL.: "Cellular effects of olomucine, an inhibitor of cyclin-dependent kinases", BIOLOGY OF THE CELL, vol. 83, no. 2-3, 1995, pages 105 - 120, XP002938419 *
HENRY M.A. ET AL.: "Oocyte maturation in rabbits: Effects of calmodulin inhibitors", ZYGOTE, vol. 5, August 1997 (1997-08-01), pages 255 - 260, XP002938421 *
LEE J. ET AL.: "Tyrosine phosphorylation of p34cdc2 in metaphase II-arrested pig oocytes results in pronucleus formation without chromosome segregation", MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 52, 1999, pages 107 - 116, XP002938418 *
SUSKO-PARRISH J.L. ET AL.: "Inhibition of protein kinases after an induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion", DEVELOPMENTAL BIOLOGY, vol. 166, 1994, pages 729 - 739 *
VESELY J. ET AL.: "Inhibition of cyclin-dependent kinases by purine analogs", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 224, 1994, pages 771 - 786, XP002938417 *

Also Published As

Publication number Publication date
WO2001030970A2 (en) 2001-05-03
AU1235401A (en) 2001-05-08

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