WO2000026357A3 - Transgenic and cloned mammals - Google Patents

Transgenic and cloned mammals Download PDF

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Publication number
WO2000026357A3
WO2000026357A3 PCT/US1999/025710 US9925710W WO0026357A3 WO 2000026357 A3 WO2000026357 A3 WO 2000026357A3 US 9925710 W US9925710 W US 9925710W WO 0026357 A3 WO0026357 A3 WO 0026357A3
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic
cloned
methods
somatic cell
mammals
Prior art date
Application number
PCT/US1999/025710
Other languages
French (fr)
Other versions
WO2000026357A2 (en
Inventor
Yann Echelard
Esmail Behbodi
Carol Ziomek
William Gavin
David Melican
Original Assignee
Genzyme Transgenic Corp
Yann Echelard
Esmail Behbodi
Carol Ziomek
William Gavin
David Melican
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/298,971 external-priority patent/US6580017B1/en
Application filed by Genzyme Transgenic Corp, Yann Echelard, Esmail Behbodi, Carol Ziomek, William Gavin, David Melican filed Critical Genzyme Transgenic Corp
Priority to JP2000579729A priority Critical patent/JP4523165B2/en
Priority to CA002350233A priority patent/CA2350233A1/en
Priority to AU14622/00A priority patent/AU1462200A/en
Priority to EP99971451A priority patent/EP1127113A2/en
Publication of WO2000026357A2 publication Critical patent/WO2000026357A2/en
Publication of WO2000026357A3 publication Critical patent/WO2000026357A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8772—Caprine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/102—Caprine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention features methods of making cloned and transgenic mammals, e.g., goats. The methods include making a somatic cell line, e.g., a transgenic somatic cell line which can be used as a donor cell, methods of producing a cloned or transgenic mammal by introducing the genome of a somatic cell into an enucleated oocyte, preferably a naturally matured oocyte which is telophase, to form a reconstructed embryo, and methods of transferring the reconstructed embryo. The invention also includes cell lines, reconstructed embryos and cloned or transgenic mammals.
PCT/US1999/025710 1998-11-02 1999-11-02 Transgenic and cloned mammals WO2000026357A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000579729A JP4523165B2 (en) 1998-11-02 1999-11-02 Transgenic and cloned mammals
CA002350233A CA2350233A1 (en) 1998-11-02 1999-11-02 Transgenic and cloned mammals
AU14622/00A AU1462200A (en) 1998-11-02 1999-11-02 Transgenic and cloned mammals
EP99971451A EP1127113A2 (en) 1998-11-02 1999-11-02 Transgenic and cloned mammals

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US10672898P 1998-11-02 1998-11-02
US29850899A 1999-04-22 1999-04-22
US09/298,971 US6580017B1 (en) 1998-11-02 1999-04-23 Methods of reconstructed goat embryo transfer
US13132899P 1999-04-26 1999-04-26
US60/106,728 1999-04-26
US09/298,971 1999-04-26
US60/131,328 1999-04-26
US09/298,508 1999-04-26

Publications (2)

Publication Number Publication Date
WO2000026357A2 WO2000026357A2 (en) 2000-05-11
WO2000026357A3 true WO2000026357A3 (en) 2000-11-23

Family

ID=27493534

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/025710 WO2000026357A2 (en) 1998-11-02 1999-11-02 Transgenic and cloned mammals

Country Status (5)

Country Link
EP (1) EP1127113A2 (en)
JP (2) JP4523165B2 (en)
AU (1) AU1462200A (en)
CA (1) CA2350233A1 (en)
WO (1) WO2000026357A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
US8030537B1 (en) * 2000-07-27 2011-10-04 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for the production of recombinant proteins, cells and organs
CN1558949A (en) * 2001-11-06 2004-12-29 �Ϻ��ڶ�ҽ�ƴ�ѧ Preparing somatic embryo by utilizing rabbit oocyte
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
CN1688190A (en) * 2002-08-01 2005-10-26 Gtc生物治疗学公司 Method for the rapid selection of homozygous primary cell lines for the production of transgenic animals by somatic cell nuclear transfer
US20120331575A1 (en) * 2009-11-18 2012-12-27 Agriculture Victoria Services Pty Ltd Transgenic non-human animals
CN104082239B (en) * 2014-06-10 2016-01-20 张海员 A kind of method for culturing special microbial strain for original ecological live pigs
FR3025515B1 (en) 2014-09-05 2016-09-09 Lab Francais Du Fractionnement PROCESS FOR PURIFYING MONOCLONAL ANTIBODY
US11246299B2 (en) 2015-03-04 2022-02-15 Pormedtec Co., Ltd. Disease model pig exhibiting stable phenotype, and production method thereof
FR3034420A1 (en) 2015-03-31 2016-10-07 Lab Francais Du Fractionnement ANTI-CD303 MONOCLONAL ANTIBODIES
WO2018033542A1 (en) 2016-08-15 2018-02-22 Danmarks Tekniske Universitet Method for reducing ammonium and lactate production in cho cells
FR3060394B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-AMHRII ANTIBODIES
FR3060395B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-HER2 ANTIBODIES
FR3076294B1 (en) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement METHOD FOR PURIFYING ANTIBODIES FROM RAW MILK
CN109315355A (en) * 2018-11-21 2019-02-12 江西和乐园生态农业科技发展有限公司 A kind of goat cultural method
WO2023118241A1 (en) 2021-12-20 2023-06-29 Vetoquinol Sa Anti-canine interleukine-31-receptor a (il-31ra) antibodies and the uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1997019589A1 (en) * 1995-11-30 1997-06-05 Nexia Biotechnologies, Inc. Method for development of transgenic goats

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996026268A1 (en) * 1995-02-21 1996-08-29 Genzyme Transgenics Corporation Transgenically produced antithrombin iii
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
WO1997019589A1 (en) * 1995-11-30 1997-06-05 Nexia Biotechnologies, Inc. Method for development of transgenic goats

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BAGUISI A, ET AL.: "Production of goats by somatic cell nuclear transfer.", NAT BIOTECHNOL, vol. 17, no. 5, May 1999 (1999-05-01), pages 456 - 461, XP000891364 *
BORDIGNON,V. & SMITH L.C.: "TELOPHASE ENUCLEATION: AN IMPROVED METHOD TO PREPARE RECIPIENT CYTOPLASTS FOR USE IN BOVINE NUCLEAR TRANSFER", MOLECULAR REPRODUCTION AND DEVELOPMENT, vol. 49, no. 1, January 1998 (1998-01-01), US,NEW YORK, pages 29 - 36, XP000910821 *
EBERT K M ET AL: "TRANSGENIC FARM ANIMALS: PROGRESS REPORT", THERIOGENOLOGY,US,LOS ALTOS, CA, vol. 39, no. 1, 1 January 1993 (1993-01-01), pages 121 - 135, XP000565804, ISSN: 0093-691X *
GOOTWINE, E. ET AL.: "Factors affecting success of embryo collection and transfer in a transgenic goat program.", THERIOGENOLOGY, (1997) VOL. 48, NO. 3, PP. 485-499., XP000892953 *
LEWIS-WILLIAMS J., ET AL.: "BIRTH OF SUCCESSFULLY IDENTIFIED TRANSGENIC GOATS USING PREIMPLANTATION STAGE EMBRYOS BIOPSIED FOR FISH.", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 47., no. 01., 1 January 1997 (1997-01-01), US, pages 226., XP000892951, ISSN: 0093-691X, DOI: 10.1016/S0093-691X(97)82353-9 *
LEWIS-WILLIAMS, J. ET AL.: "Birth of successfully identified transgenic goats using preimplantation stage embryos biopsied for fish.", THERIOGENOLOGY, (1997) VOL. 47, NO. 1, PP. 226. *
WILMUT I ET AL: "VIABLE OFFSPRING DERIVED FROM FETAL AND ADULT MAMMALIAN CELLS", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 385, no. 6619, 27 February 1997 (1997-02-27), pages 810 - 813, XP002067035, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
JP4523165B2 (en) 2010-08-11
JP2004073189A (en) 2004-03-11
WO2000026357A2 (en) 2000-05-11
CA2350233A1 (en) 2000-05-11
AU1462200A (en) 2000-05-22
EP1127113A2 (en) 2001-08-29
JP2002528117A (en) 2002-09-03
JP4068516B2 (en) 2008-03-26

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