WO2001014590A3 - Simultaneous isolation and quantitation of dna - Google Patents

Simultaneous isolation and quantitation of dna Download PDF

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Publication number
WO2001014590A3
WO2001014590A3 PCT/US2000/022827 US0022827W WO0114590A3 WO 2001014590 A3 WO2001014590 A3 WO 2001014590A3 US 0022827 W US0022827 W US 0022827W WO 0114590 A3 WO0114590 A3 WO 0114590A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna target
target material
methods
dna
complex
Prior art date
Application number
PCT/US2000/022827
Other languages
French (fr)
Other versions
WO2001014590A2 (en
Inventor
Allan M Tereba
Rex M Bitner
Susan C Koller
Craig E Smith
Daniel D Kephart
Steven J Ekenberg
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/377,986 external-priority patent/US6673631B1/en
Application filed by Promega Corp filed Critical Promega Corp
Priority to CA002379503A priority Critical patent/CA2379503A1/en
Priority to DE60015148T priority patent/DE60015148T2/en
Priority to JP2001518457A priority patent/JP4425513B2/en
Priority to EP00955744A priority patent/EP1204741B1/en
Priority to AT00955744T priority patent/ATE280225T1/en
Priority to AU67895/00A priority patent/AU780319B2/en
Publication of WO2001014590A2 publication Critical patent/WO2001014590A2/en
Publication of WO2001014590A3 publication Critical patent/WO2001014590A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention provides methods for isolating a defined quantity of DNA target material from other substances in a medium. The method may be carried out using a known quantity of a silica-containing solid support, such as silica magnetic particles, having a definable capacity for reversibly binding DNA target material, and DNA target material in excess of the binding capacity of the particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the DNA target material in a mixture of the medium and particles, and separating the complex from the mixture using external magnetic force. The DNA target material may then be eluted from the complex. The quantity of DNA target material eluted may be determined based on a calibration model. The methods of the present invention permit isolation of DNA target material which is within a known quantity range. The methods of the invention eliminate the step of quantitating purified biological samples prior to further processing, such as amplification, Short Tandem Repeat (STR) analysis, and DNA sequencing. Samples of the DNA target materials may be obtained from liquid or solid media, such as liquid blood or paper.
PCT/US2000/022827 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna WO2001014590A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002379503A CA2379503A1 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna
DE60015148T DE60015148T2 (en) 1999-08-20 2000-08-18 SIMULTANEOUS ISOLATION AND QUANTIFICATION OF DNA
JP2001518457A JP4425513B2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantification of DNA
EP00955744A EP1204741B1 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna
AT00955744T ATE280225T1 (en) 1999-08-20 2000-08-18 SIMULTANEOUS ISOLATION AND QUANTIFICATION OF DNA
AU67895/00A AU780319B2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of DNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/377,986 1999-08-20
US09/377,986 US6673631B1 (en) 1997-01-21 1999-08-20 Simultaneous isolation and quantitation of DNA

Publications (2)

Publication Number Publication Date
WO2001014590A2 WO2001014590A2 (en) 2001-03-01
WO2001014590A3 true WO2001014590A3 (en) 2001-10-11

Family

ID=23491269

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/022827 WO2001014590A2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna

Country Status (8)

Country Link
EP (1) EP1204741B1 (en)
JP (1) JP4425513B2 (en)
CN (1) CN1370230A (en)
AT (1) ATE280225T1 (en)
AU (1) AU780319B2 (en)
CA (1) CA2379503A1 (en)
DE (1) DE60015148T2 (en)
WO (1) WO2001014590A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8026068B2 (en) 2002-01-08 2011-09-27 Roche Molecular Systems, Inc. Use of silica material in an amplification reaction

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE449186T1 (en) 2001-11-28 2009-12-15 Applied Biosystems Llc COMPOSITIONS AND METHODS FOR SELECTIVE NUCLEIC ACID ISOLATION
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1621890A1 (en) * 2004-07-26 2006-02-01 bioMerieux B.V. Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods
EP1690938A1 (en) * 2005-02-11 2006-08-16 Qiagen GmbH Method for isolating nucleic acids, wherein the nucleic acids are immobilised on a matrix at elevated temperatures
KR101163425B1 (en) 2006-04-14 2012-07-13 닛본 덴끼 가부시끼가이샤 Individual discrimination method and apparatus
JP2009065900A (en) * 2007-09-13 2009-04-02 Olympus Corp Methods for analyzing and preserving target rna
JP2011504592A (en) * 2007-11-26 2011-02-10 アトノミックス アクティーゼルスカブ Integrated separation and detection cartridge with magnetic particles having a bimodal size distribution
DE102008047790A1 (en) * 2008-09-17 2010-04-15 Qiagen Gmbh Method for normalizing the content of biomolecules in a sample
CN102667481A (en) * 2009-12-23 2012-09-12 皇家飞利浦电子股份有限公司 Analyte measurement apparatus and method
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN101818143A (en) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 Ultra-low amount DNA extraction kit and method based on silica magnetic compound particles
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
DE112011102524T5 (en) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Preparation of generic samples
ES2642862T3 (en) * 2012-01-25 2017-11-20 Gencell Biosystems Limited Isolation of biomolecules
CA2868805C (en) * 2012-03-28 2018-09-04 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
CN104583397B (en) * 2012-08-28 2018-05-25 生物立方体系统有限公司 For being rapidly separated porous solid phase of nucleic acid amplification reaction biomolecule and application thereof from biological sample
CN105603056B (en) * 2014-11-21 2020-09-25 丰益(上海)生物技术研发中心有限公司 Vegetable oil doping detection method
CN104560952A (en) * 2014-12-29 2015-04-29 福建师范大学 Method for rapidly extracting DNA of gram-positive bacterium genome based on paramagnetic particle method
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
JP2018526021A (en) * 2015-09-11 2018-09-13 コーニング インコーポレイテッド Compositions and methods for purification of nucleic acids from blood samples
WO2018128585A1 (en) * 2017-01-04 2018-07-12 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
CN109321634A (en) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 Nucleic acid uniforms method and its kit and application
WO2019035335A1 (en) * 2017-08-18 2019-02-21 Ａｇｃ株式会社 Nucleic acid collection method, nucleic-acid-binding carrier, and nucleic acid collection kit
US20230159912A1 (en) * 2021-11-23 2023-05-25 Bioo Scientific Corporation Compositions and methods for nucleic acid normalization

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031840A1 (en) * 1997-01-21 1998-07-23 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031840A1 (en) * 1997-01-21 1998-07-23 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8026068B2 (en) 2002-01-08 2011-09-27 Roche Molecular Systems, Inc. Use of silica material in an amplification reaction

Also Published As

Publication number Publication date
EP1204741B1 (en) 2004-10-20
CN1370230A (en) 2002-09-18
JP4425513B2 (en) 2010-03-03
DE60015148T2 (en) 2006-02-16
ATE280225T1 (en) 2004-11-15
AU6789500A (en) 2001-03-19
CA2379503A1 (en) 2001-03-01
AU780319B2 (en) 2005-03-17
WO2001014590A2 (en) 2001-03-01
EP1204741A2 (en) 2002-05-15
DE60015148D1 (en) 2004-11-25
JP2003507049A (en) 2003-02-25

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