WO2001012827A3 - Method for production of transgenic plants with increased tocopherol content - Google Patents

Method for production of transgenic plants with increased tocopherol content Download PDF

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Publication number
WO2001012827A3
WO2001012827A3 PCT/EP2000/007807 EP0007807W WO0112827A3 WO 2001012827 A3 WO2001012827 A3 WO 2001012827A3 EP 0007807 W EP0007807 W EP 0007807W WO 0112827 A3 WO0112827 A3 WO 0112827A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
transgenic plants
tocopherol content
nucleic acid
increased tocopherol
Prior art date
Application number
PCT/EP2000/007807
Other languages
German (de)
French (fr)
Other versions
WO2001012827A2 (en
WO2001012827A9 (en
Inventor
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Original Assignee
Sungene Gmbh & Co Kgaa
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa, Marcus Ebneth, Karin Herbers, Michael Geiger, Isolde Saalbach filed Critical Sungene Gmbh & Co Kgaa
Priority to EP00951492A priority Critical patent/EP1200598A2/en
Priority to BR0013127-0A priority patent/BR0013127A/en
Priority to CA002381316A priority patent/CA2381316A1/en
Priority to AU64409/00A priority patent/AU6440900A/en
Publication of WO2001012827A2 publication Critical patent/WO2001012827A2/en
Publication of WO2001012827A3 publication Critical patent/WO2001012827A3/en
Publication of WO2001012827A9 publication Critical patent/WO2001012827A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a new type of expression cassettes which, under genetic control, contain regulating nucleic acid sequences a) nucleic acid sequence coding for 4-hydrophenylpyruvate dioxygenase (HPPD) or for one of its functional equivalents; and/or b) at least one nucleic acid sequence (anti-HGD), which can inhibit the homogentisate-dioxygenase (HGD) activity. The invention also relates to vectors which are suitable for the production of plants having an increased tocopherol content, to transgenic plants produced therewith, and to a method for the production of transgenic plants having an increased tocopherol content.
PCT/EP2000/007807 1999-08-11 2000-08-10 Method for production of transgenic plants with increased tocopherol content WO2001012827A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00951492A EP1200598A2 (en) 1999-08-11 2000-08-10 Method for production of transgenic plants with increased tocopherol content
BR0013127-0A BR0013127A (en) 1999-08-11 2000-08-10 Expression cassette, recombinant vector, microorganism, transgenic plant, method for generating transgenic plants, uses of a microorganism vector, an expression cassette, or a transgenic plant, and process for the preparation of tocopherols
CA002381316A CA2381316A1 (en) 1999-08-11 2000-08-10 Homogentisate-dioxygenase
AU64409/00A AU6440900A (en) 1999-08-11 2000-08-10 Homogentisate-dioxygenase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19937957A DE19937957A1 (en) 1999-08-11 1999-08-11 Homogenate dioxygenase
DE19937957.2 1999-08-11

Publications (3)

Publication Number Publication Date
WO2001012827A2 WO2001012827A2 (en) 2001-02-22
WO2001012827A3 true WO2001012827A3 (en) 2001-08-23
WO2001012827A9 WO2001012827A9 (en) 2002-05-10

Family

ID=7917993

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007807 WO2001012827A2 (en) 1999-08-11 2000-08-10 Method for production of transgenic plants with increased tocopherol content

Country Status (7)

Country Link
EP (1) EP1200598A2 (en)
AR (1) AR029762A1 (en)
AU (1) AU6440900A (en)
BR (1) BR0013127A (en)
CA (1) CA2381316A1 (en)
DE (1) DE19937957A1 (en)
WO (1) WO2001012827A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0010616A (en) 1999-04-15 2002-05-07 Calgene Llc Nucleic acid sequences to proteins involved in isoprenoid synthesis
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
WO2002012478A2 (en) 2000-08-07 2002-02-14 Monsanto Technology, Llc Methyl-d-erythritol phosphate pathway genes
DE10046462A1 (en) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Improved procedures for vitamin E biosynthesis
JP2004533244A (en) 2001-05-09 2004-11-04 モンサント テクノロジー リミテッド ライアビリティー カンパニー TyrA gene and uses thereof
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
CN100400661C (en) 2001-10-25 2008-07-09 孟山都技术有限公司 Aromatic methyltransferases and uses thereof
CA2478957C (en) 2002-03-19 2013-07-02 Monsanto Technology, Llc Homogentisate prenyl transferase ("hpt") nucleic acids and polypeptides, and uses thereof
CN1681928A (en) 2002-08-05 2005-10-12 孟山都技术公司 Tocopherol biosynthesis related genes and uses thereof
GB2595633A (en) * 2020-04-15 2021-12-08 Univ Liverpool Anti-infective agents

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004685A1 (en) * 1996-07-25 1998-02-05 American Cyanamid Company Hppd gene and inhibitors
WO1999004021A1 (en) * 1997-07-14 1999-01-28 Basf Aktiengesellschaft Dna sequence coding for a hydroxyphenylpyruvate dioxygenase and overproduction thereof in plants
FR2778527A1 (en) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Producing tomato, cereal or oilseed plants, especially maize, oilseed rape, soya or sunflower plants, from which tocopherols can be extracted and purified
WO2000008169A1 (en) * 1998-08-05 2000-02-17 Sungene Gmbh & Co.Kgaa Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004685A1 (en) * 1996-07-25 1998-02-05 American Cyanamid Company Hppd gene and inhibitors
WO1999004021A1 (en) * 1997-07-14 1999-01-28 Basf Aktiengesellschaft Dna sequence coding for a hydroxyphenylpyruvate dioxygenase and overproduction thereof in plants
FR2778527A1 (en) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Producing tomato, cereal or oilseed plants, especially maize, oilseed rape, soya or sunflower plants, from which tocopherols can be extracted and purified
WO2000008169A1 (en) * 1998-08-05 2000-02-17 Sungene Gmbh & Co.Kgaa Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 6 January 1999 (1999-01-06), SCHMIDT S.R. ET AL.: "Arabidopsis thaliana homogentisate 1,2-dioxygenase mRNA, complete cds", XP002162586 *
DENOYA C D ET AL: "A STREPTOMYCES AVERMITILIS GENE ENCODING A 4-HYDROXYPHENYLPYRUVIC ACID DIOXYGENASE-LIKE PROTEIN THAT DIRECTS THE PRODUCTION OF HOMOGENTISIC ACID AND AN OCHRONOTIC PIGMENT IN ESCHERICHIA COLI", JOURNAL OF BACTERIOLOGY,US,WASHINGTON, DC, vol. 176, no. 17, 1 September 1994 (1994-09-01), pages 5312 - 5319, XP002028042, ISSN: 0021-9193 *
NEWMAN T ET AL: "GENES GALORE: A SUMMARY OF METHODS FOR ACCESSING RESULTS FROM LARGE-SCALE PARTIAL SEQUENCING OF ANONYMOUS ARABIDOPSIS CDNA CLONES", PLANT PHYSIOLOGY,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 106, 1994, pages 1241 - 1255, XP000571449 *

Also Published As

Publication number Publication date
DE19937957A1 (en) 2001-02-15
WO2001012827A2 (en) 2001-02-22
CA2381316A1 (en) 2001-02-22
EP1200598A2 (en) 2002-05-02
AR029762A1 (en) 2003-07-16
BR0013127A (en) 2002-04-23
WO2001012827A9 (en) 2002-05-10
AU6440900A (en) 2001-03-13

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