WO2001011087A3 - Technique permettant d'evaluer un vecteur de transfert des genes, et les interactions de produits vectoriels de transfert de genes - Google Patents

Technique permettant d'evaluer un vecteur de transfert des genes, et les interactions de produits vectoriels de transfert de genes Download PDF

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Publication number
WO2001011087A3
WO2001011087A3 PCT/US2000/022234 US0022234W WO0111087A3 WO 2001011087 A3 WO2001011087 A3 WO 2001011087A3 US 0022234 W US0022234 W US 0022234W WO 0111087 A3 WO0111087 A3 WO 0111087A3
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WO
WIPO (PCT)
Prior art keywords
gene transfer
transfer vector
vector
evaluating
charged fragments
Prior art date
Application number
PCT/US2000/022234
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English (en)
Other versions
WO2001011087A2 (fr
Inventor
Miguel Carrion
Duncan L Mcvey
Imre Kovesdi
Original Assignee
Genvec Inc
Miguel Carrion
Duncan L Mcvey
Imre Kovesdi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genvec Inc, Miguel Carrion, Duncan L Mcvey, Imre Kovesdi filed Critical Genvec Inc
Priority to AU65396/00A priority Critical patent/AU6539600A/en
Publication of WO2001011087A2 publication Critical patent/WO2001011087A2/fr
Publication of WO2001011087A3 publication Critical patent/WO2001011087A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Optics & Photonics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une technique permettant d'évaluer un vecteur de transfert de gènes, ainsi que des interactions de vecteurs de transfert de gènes. Ladite technique consiste à fournir un vecteur de transfert de gènes comprenant un gène exogène codant pour un produit vectoriel de transfert de gènes, à introduire ledit vecteur de transfert de gènes dans une cellule, nécessaire à l'expression du gène exogène et à la production du produit vectoriel de transfert de gènes, afin de former une cellule transduite, et à exprimer ce gène exogène de façon à produire le produit vectoriel de transfert de gènes. Des fragments chargés sont produits à partir du produit vectoriel de transfert de gènes. Un détecteur détecte lesdits fragments chargés, génère un signal échantillon correspondant au rapport masse/charge des fragments chargé, et comprend des informations caractéristiques du produit vectoriel de transfert de gènes. Le vecteur de transfert de gènes est évalué par évaluation du signal échantillon. L'invention concerne également une technique d'évaluation des interactions d'un vecteur avec des molécules interactives. Ce vecteur peut s'associer avec au moins une molécule potentiellement interactive, de sorte qu'un complexe molécules interactives/vecteur se forme lorsqu'une molécule interactive s'associe audit vecteur. Ladite technique concerne également la production de fragments chargés à partir du vecteur, et éventuellement, si un complexe est formé, à partir de la molécule interactive. Un détecteur détecte les fragments chargés et produit un signal échantillon correspondant au rapport masse/charge des fragments chargés, et comprenant des informations caractéristiques du vecteur, et éventuellement, si un complexe est formé, des caractéristiques de la molécule interactive. Les interactions du vecteur avec les molécules interactives sont ensuite évaluées par évaluation du signal échantillon.
PCT/US2000/022234 1999-08-10 2000-08-10 Technique permettant d'evaluer un vecteur de transfert des genes, et les interactions de produits vectoriels de transfert de genes WO2001011087A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU65396/00A AU6539600A (en) 1999-08-10 2000-08-10 Method of evaluating a gene transfer vector and gene transfer vector product interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37168499A 1999-08-10 1999-08-10
US09/371,684 1999-08-10

Publications (2)

Publication Number Publication Date
WO2001011087A2 WO2001011087A2 (fr) 2001-02-15
WO2001011087A3 true WO2001011087A3 (fr) 2002-07-11

Family

ID=23464996

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/022234 WO2001011087A2 (fr) 1999-08-10 2000-08-10 Technique permettant d'evaluer un vecteur de transfert des genes, et les interactions de produits vectoriels de transfert de genes

Country Status (2)

Country Link
AU (1) AU6539600A (fr)
WO (1) WO2001011087A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002004628A2 (fr) * 2000-07-06 2002-01-17 Genvec, Inc. Procede d'identification d'un produit genique
US6972611B1 (en) 2000-07-13 2005-12-06 Ct Concept Technologie Ag Method and device for a state-dependent control of the transient behavior of semiconductor power switches
CN110501409A (zh) * 2019-08-28 2019-11-26 武汉金开瑞生物工程有限公司 一种蛋白质除盐方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965358A (en) * 1998-08-26 1999-10-12 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965358A (en) * 1998-08-26 1999-10-12 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks
WO2000012765A1 (fr) * 1998-08-26 2000-03-09 Genvec, Inc. Procede d'evaluation de la purete relative des populations de vecteurs viraux de transfert de genes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
LAMOND AND MANN: "Cell biology and the genome projects - a concerted strategy for characterizing multiprotein complexes by using mass spectrometry", TRENDS IN CELL BIOLOGY, vol. 7, April 1997 (1997-04-01), pages 139 - 142, XP001041761 *
LINK ET AL.: "Direct analysis of protein complexes using mass spectrometry", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 676 - 682, XP002184653 *
MCCORMACK ET AL.: "Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level", ANALYTICAL CHEMISTRY, vol. 69, 15 February 1997 (1997-02-15), pages 767 - 776, XP002184654 *
SIUZDAK: "Probing viruses with Mass Spectrometry", JOURNAL OF MASS SPECTROMETRY, vol. 33, March 1998 (1998-03-01), pages 203 - 211, XP000863342 *
SÖNKSEN ET AL.: "Combining MALDI Mass Spectrometry and Biomolecular Interaction Analysis Using a Biomolecular Interaction Analysis Instrument", ANALYTICAL CHEMISTRY, vol. 70, no. 13, 1 July 1998 (1998-07-01), pages 2731 - 2736, XP002184655 *
YATES, J.R.: "Mass spectrometry from genomics to proteomics", TRENDS IN GENETICS, vol. 16, no. 1, January 2000 (2000-01-01), pages 5 - 8, XP004186477 *

Also Published As

Publication number Publication date
AU6539600A (en) 2001-03-05
WO2001011087A2 (fr) 2001-02-15

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