WO2001011087A3 - Method of evaluating a gene transfer vector and gene transfer vector product interactions - Google Patents

Method of evaluating a gene transfer vector and gene transfer vector product interactions Download PDF

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Publication number
WO2001011087A3
WO2001011087A3 PCT/US2000/022234 US0022234W WO0111087A3 WO 2001011087 A3 WO2001011087 A3 WO 2001011087A3 US 0022234 W US0022234 W US 0022234W WO 0111087 A3 WO0111087 A3 WO 0111087A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene transfer
transfer vector
vector
evaluating
charged fragments
Prior art date
Application number
PCT/US2000/022234
Other languages
French (fr)
Other versions
WO2001011087A2 (en
Inventor
Miguel Carrion
Duncan L Mcvey
Imre Kovesdi
Original Assignee
Genvec Inc
Miguel Carrion
Duncan L Mcvey
Imre Kovesdi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genvec Inc, Miguel Carrion, Duncan L Mcvey, Imre Kovesdi filed Critical Genvec Inc
Priority to AU65396/00A priority Critical patent/AU6539600A/en
Publication of WO2001011087A2 publication Critical patent/WO2001011087A2/en
Publication of WO2001011087A3 publication Critical patent/WO2001011087A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Abstract

The present invention provides a method of evaluating a gene transfer vector as well as gene transfer vector interactions. The method comprises providing a gene transfer vector including an exogenous gene encoding a gene transfer vector product, inserting the gene transfer vector into a cell, permissive for expression of the exogenous gene and production of the gene transfer vector product, to form a transduced cell, and expressing the exogenous gene to produce the gene transfer vector product. Charged fragments are produced from the gene transfer vector product, and a detector detects the charged fragments and produces a sample signal corresponding to the mass-to-charge ratio of the charged fragments and includes information characteristic of the gene transfer vector product. The gene transfer vector is evaluated by evaluating the sample signal. The present invention also provides a method of evaluating a vector for interactions with interacting molecules. The vector is permitted to associate with one or more potentially interacting molecules, such that a vector-interacting molecule complex will form if a potentially interacting molecule associates with the vector. The method further calls for producing charged fragments from the vector, and, if a complex is formed, the interacting molecule, detecting the charged fragments with a detector that produces a sample signal corresponding to the mass-to-charge ratio of the charged fragments and comprising information characteristic of the vector, and, if a complex is formed, optionally characteristic of the interacting molecule. The vector's interactions with interacting molecules then are evaluated by evaluation of the sample signal.
PCT/US2000/022234 1999-08-10 2000-08-10 Method of evaluating a gene transfer vector and gene transfer vector product interactions WO2001011087A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU65396/00A AU6539600A (en) 1999-08-10 2000-08-10 Method of evaluating a gene transfer vector and gene transfer vector product interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37168499A 1999-08-10 1999-08-10
US09/371,684 1999-08-10

Publications (2)

Publication Number Publication Date
WO2001011087A2 WO2001011087A2 (en) 2001-02-15
WO2001011087A3 true WO2001011087A3 (en) 2002-07-11

Family

ID=23464996

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/022234 WO2001011087A2 (en) 1999-08-10 2000-08-10 Method of evaluating a gene transfer vector and gene transfer vector product interactions

Country Status (2)

Country Link
AU (1) AU6539600A (en)
WO (1) WO2001011087A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001278872A1 (en) 2000-07-06 2002-01-21 Genvec, Inc. Method of identifying a binding partner of a gene product
DE50015173D1 (en) 2000-07-13 2008-07-03 Ct Concept Technologie Ag METHOD AND DEVICE FOR STATUS-RELATED CONTROL OF THE TRANSIENT BEHAVIOR OF POWER SIGNAL SWITCHES
CN110501409A (en) * 2019-08-28 2019-11-26 武汉金开瑞生物工程有限公司 A kind of protein desalination method and its application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965358A (en) * 1998-08-26 1999-10-12 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965358A (en) * 1998-08-26 1999-10-12 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks
WO2000012765A1 (en) * 1998-08-26 2000-03-09 Genvec, Inc. Method for assessing the relative purity of viral gene transfer vector stocks

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
LAMOND AND MANN: "Cell biology and the genome projects - a concerted strategy for characterizing multiprotein complexes by using mass spectrometry", TRENDS IN CELL BIOLOGY, vol. 7, April 1997 (1997-04-01), pages 139 - 142, XP001041761 *
LINK ET AL.: "Direct analysis of protein complexes using mass spectrometry", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 676 - 682, XP002184653 *
MCCORMACK ET AL.: "Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level", ANALYTICAL CHEMISTRY, vol. 69, 15 February 1997 (1997-02-15), pages 767 - 776, XP002184654 *
SIUZDAK: "Probing viruses with Mass Spectrometry", JOURNAL OF MASS SPECTROMETRY, vol. 33, March 1998 (1998-03-01), pages 203 - 211, XP000863342 *
SÖNKSEN ET AL.: "Combining MALDI Mass Spectrometry and Biomolecular Interaction Analysis Using a Biomolecular Interaction Analysis Instrument", ANALYTICAL CHEMISTRY, vol. 70, no. 13, 1 July 1998 (1998-07-01), pages 2731 - 2736, XP002184655 *
YATES, J.R.: "Mass spectrometry from genomics to proteomics", TRENDS IN GENETICS, vol. 16, no. 1, January 2000 (2000-01-01), pages 5 - 8, XP004186477 *

Also Published As

Publication number Publication date
AU6539600A (en) 2001-03-05
WO2001011087A2 (en) 2001-02-15

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