WO2000079274A3 - Methods for assaying subcellular conditions using energy transfer - Google Patents

Methods for assaying subcellular conditions using energy transfer Download PDF

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Publication number
WO2000079274A3
WO2000079274A3 PCT/US2000/017380 US0017380W WO0079274A3 WO 2000079274 A3 WO2000079274 A3 WO 2000079274A3 US 0017380 W US0017380 W US 0017380W WO 0079274 A3 WO0079274 A3 WO 0079274A3
Authority
WO
WIPO (PCT)
Prior art keywords
energy transfer
methods
assaying
membrane potential
subcellular
Prior art date
Application number
PCT/US2000/017380
Other languages
French (fr)
Other versions
WO2000079274A2 (en
Inventor
James A Dykens
Gonul Velicelebi
Soumitra S Ghosh
Original Assignee
Mitokor
James A Dykens
Gonul Velicelebi
Soumitra S Ghosh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/338,122 external-priority patent/US6323039B1/en
Application filed by Mitokor, James A Dykens, Gonul Velicelebi, Soumitra S Ghosh filed Critical Mitokor
Priority to AU57636/00A priority Critical patent/AU5763600A/en
Priority to JP2001505191A priority patent/JP2003506014A/en
Priority to EP00943119A priority patent/EP1210596A2/en
Priority to CA002375542A priority patent/CA2375542A1/en
Publication of WO2000079274A2 publication Critical patent/WO2000079274A2/en
Publication of WO2000079274A3 publication Critical patent/WO2000079274A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G01N33/5079—Mitochondria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention provides compositions and methods for monitoring subcellular compartments such as organelles by energy transfer techniques that do not require specific intermolecular affinity binding events between energy transfer donor and energy transfer acceptor molecules. Provided are methods for assaying cellular membrane potential, including mitochondrial membrane potential, by energy transfer methodologies including fluorescence resonance energy transfer (FRET). Diagnostic and drug screening assays are also provided.
PCT/US2000/017380 1999-06-22 2000-06-22 Methods for assaying subcellular conditions using energy transfer WO2000079274A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU57636/00A AU5763600A (en) 1999-06-22 2000-06-22 Compositions and methods for assaying subcellular conditions and processes usingenergy transfer
JP2001505191A JP2003506014A (en) 1999-06-22 2000-06-22 Compositions and methods for assaying subcellular conditions and processes using energy transfer
EP00943119A EP1210596A2 (en) 1999-06-22 2000-06-22 Methods for assaying subcellular conditions using energy transfer
CA002375542A CA2375542A1 (en) 1999-06-22 2000-06-22 Compositions and methods for assaying subcellular conditions and processes energy transfer

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US14043399P 1999-06-22 1999-06-22
US09/338,122 US6323039B1 (en) 1999-06-22 1999-06-22 Compositions and methods for assaying subcellular conditions and processes using energy transfer
US60/140,433 1999-06-22
US09/338,122 1999-06-22
US17638300P 2000-01-14 2000-01-14
US60/176,383 2000-01-14

Publications (2)

Publication Number Publication Date
WO2000079274A2 WO2000079274A2 (en) 2000-12-28
WO2000079274A3 true WO2000079274A3 (en) 2002-01-10

Family

ID=27385499

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/017380 WO2000079274A2 (en) 1999-06-22 2000-06-22 Methods for assaying subcellular conditions using energy transfer

Country Status (5)

Country Link
EP (1) EP1210596A2 (en)
JP (1) JP2003506014A (en)
AU (1) AU5763600A (en)
CA (1) CA2375542A1 (en)
WO (1) WO2000079274A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ296799A0 (en) 1999-09-20 1999-10-14 Unisearch Limited A cell-membrane impermeable trivalent organoarsenical derivative and use thereof
US6287758B1 (en) * 2000-03-23 2001-09-11 Axiom Biotechnologies, Inc. Methods of registering trans-membrane electric potentials
AUPR537101A0 (en) * 2001-05-31 2001-06-28 International Diabetes Institute A method of assessment and treatment
EP1423122A4 (en) * 2001-08-15 2008-12-10 Univ Michigan Compositions and methods relating to novel benzodiazepine compounds and targets thereof
EP1563099B1 (en) 2002-11-07 2009-07-22 Newsouth Innovations Pty Limited Induction of the mitochondrial permeability transition
JP4755587B2 (en) 2004-05-18 2011-08-24 田辺三菱製薬株式会社 Method for evaluating substances that change the membrane potential of mitochondria
US8268883B2 (en) 2006-11-01 2012-09-18 Newsouth Innovations Pty Limited Organo-arsenoxide compounds and use thereof
JP5683967B2 (en) 2009-02-06 2015-03-11 五稜化学株式会社 Fluorescent solvatochromic dye
WO2011118579A1 (en) * 2010-03-25 2011-09-29 国立大学法人富山大学 Fluorescent probe for plasma cell identification and isolation, and plasma cell identification or isolation method using the probe
EP2995948A1 (en) * 2014-09-09 2016-03-16 Ecole Polytechnique Federale de Lausanne (EPFL) Methods and compounds useful in hematopoietic stem cell medicine
JP6958860B2 (en) 2017-11-07 2021-11-02 学校法人自治医科大学 Agents for improving mitochondrial dysfunction, preventive or therapeutic agents for diseases or symptoms caused by mitochondrial dysfunction, and their uses

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041166A2 (en) * 1995-06-07 1996-12-19 The Regents Of The University Of California Detection of transmembrane potentials by optical methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041166A2 (en) * 1995-06-07 1996-12-19 The Regents Of The University Of California Detection of transmembrane potentials by optical methods

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CACCIATORE TIMOTHY W ET AL: "Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes.", NEURON, vol. 23, no. 3, July 1999 (1999-07-01), pages 449 - 459, XP000961663, ISSN: 0896-6273 *
CORTESE JORGE D ET AL: "Novel fluorescence membrane fusion assays reveal GTP-dependent fusogenic properties of outer mitochondrial membrane-derived proteins.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1371, no. 2, 28 May 1998 (1998-05-28), pages 185 - 198, XP000961685, ISSN: 0006-3002 *
DYKENS J A ET AL: "A FRET-based, whole-cell assay of mitochondrial membrane potential for drug screening.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 25, no. 1-2, 1999, 29th Annual Meeting of the Society for Neuroscience, Part 1;Miami Beach, Florida, USA; October 23-28, 1999, pages 282, XP000961632, ISSN: 0190-5295 *
GONZALEZ JESUS E ET AL: "Improved indicators of cell membrane potential that use fluorescence resonance energy transfer.", CHEMISTRY & BIOLOGY (LONDON), vol. 4, no. 4, 1997, pages 269 - 277, XP000961796, ISSN: 1074-5521 *
GONZALEZ JESUS E ET AL: "Voltage sensing by fluorescence resonance energy transfer in single cells.", BIOPHYSICAL JOURNAL, vol. 69, no. 4, 1995, pages 1272 - 1280, XP000961694, ISSN: 0006-3495 *
HUEGLIN D ET AL: "Time-resolved microfluorometric study of the binding sites of lipophilic cationic pyrene probes in mitochondria of living HeLa cells.", JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B BIOLOGY, vol. 31, no. 3, 1995, pages 145 - 158, XP000998251, ISSN: 1011-1344 *
LEMASTERS JOHN J ET AL: "The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis and autophagy.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1366, no. 1-2, 10 August 1998 (1998-08-10), pages 177 - 196, XP000961686, ISSN: 0006-3002 *
MAHAJAN N P ET AL: "BCL-2 AND BAX INTERACTIONS IN MITOCHONDRIA PROBED WITH GREEN FLUORESCENT PROTEIN AND FLUORESCENCE RESONANCE ENERGY TRANSFER", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 16, June 1998 (1998-06-01), pages 547 - 552, XP000876894, ISSN: 1087-0156 *
RIZZUTO R ET AL: "DOUBLE LABELLING OF SUBCELLULAR STRUCTURES WITH ORGANELLE-TARGETED GFP MUTANTS IN VIVO", CURRENT BIOLOGY,GB,CURRENT SCIENCE,, vol. 6, no. 2, 1 February 1996 (1996-02-01), pages 183 - 188, XP000610252, ISSN: 0960-9822 *
USTER, PAUL S. ET AL: "Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells", J. CELL BIOL. (1986), 103(4), 1221-34, XP000998288 *

Also Published As

Publication number Publication date
AU5763600A (en) 2001-01-09
EP1210596A2 (en) 2002-06-05
WO2000079274A2 (en) 2000-12-28
JP2003506014A (en) 2003-02-18
CA2375542A1 (en) 2000-12-28

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