WO2000079274A3 - Methods for assaying subcellular conditions using energy transfer - Google Patents
Methods for assaying subcellular conditions using energy transfer Download PDFInfo
- Publication number
- WO2000079274A3 WO2000079274A3 PCT/US2000/017380 US0017380W WO0079274A3 WO 2000079274 A3 WO2000079274 A3 WO 2000079274A3 US 0017380 W US0017380 W US 0017380W WO 0079274 A3 WO0079274 A3 WO 0079274A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- energy transfer
- methods
- assaying
- membrane potential
- subcellular
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
- G01N33/5079—Mitochondria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00943119A EP1210596A2 (en) | 1999-06-22 | 2000-06-22 | Methods for assaying subcellular conditions using energy transfer |
AU57636/00A AU5763600A (en) | 1999-06-22 | 2000-06-22 | Compositions and methods for assaying subcellular conditions and processes usingenergy transfer |
CA002375542A CA2375542A1 (en) | 1999-06-22 | 2000-06-22 | Compositions and methods for assaying subcellular conditions and processes energy transfer |
JP2001505191A JP2003506014A (en) | 1999-06-22 | 2000-06-22 | Compositions and methods for assaying subcellular conditions and processes using energy transfer |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14043399P | 1999-06-22 | 1999-06-22 | |
US60/140,433 | 1999-06-22 | ||
US09/338,122 US6323039B1 (en) | 1999-06-22 | 1999-06-22 | Compositions and methods for assaying subcellular conditions and processes using energy transfer |
US09/338,122 | 1999-06-22 | ||
US17638300P | 2000-01-14 | 2000-01-14 | |
US60/176,383 | 2000-01-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000079274A2 WO2000079274A2 (en) | 2000-12-28 |
WO2000079274A3 true WO2000079274A3 (en) | 2002-01-10 |
Family
ID=27385499
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/017380 WO2000079274A2 (en) | 1999-06-22 | 2000-06-22 | Methods for assaying subcellular conditions using energy transfer |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1210596A2 (en) |
JP (1) | JP2003506014A (en) |
AU (1) | AU5763600A (en) |
CA (1) | CA2375542A1 (en) |
WO (1) | WO2000079274A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPQ296799A0 (en) | 1999-09-20 | 1999-10-14 | Unisearch Limited | A cell-membrane impermeable trivalent organoarsenical derivative and use thereof |
US6287758B1 (en) * | 2000-03-23 | 2001-09-11 | Axiom Biotechnologies, Inc. | Methods of registering trans-membrane electric potentials |
AUPR537101A0 (en) * | 2001-05-31 | 2001-06-28 | International Diabetes Institute | A method of assessment and treatment |
WO2003015703A2 (en) * | 2001-08-15 | 2003-02-27 | The Regents Of The University Of Michigan | Compositions and methods relating to novel benzodiazepine compounds and targets thereof |
ES2330519T3 (en) | 2002-11-07 | 2009-12-11 | Newsouth Innovations Pty Limited | MITOCONDRIAL PERMEABILITY TRANSITION INDICATION. |
EP1760158B1 (en) * | 2004-05-18 | 2013-07-17 | Mitsubishi Tanabe Pharma Corporation | Method of assaying substance capable of changing mitochondrial membrane potential |
EP2069371B1 (en) | 2006-11-01 | 2013-03-13 | NewSouth Innovations Pty Limited | Organo-arsenoxide compounds and use thereof |
CN102439091A (en) | 2009-02-06 | 2012-05-02 | 国立大学法人北海道大学 | Fluorescent solvatochromic pigment |
EP2551352B1 (en) * | 2010-03-25 | 2016-09-14 | National University Corporation University Of Toyama | Fluorescent probe for plasma cell identification and isolation, and plasma cell identification or isolation method using the probe |
EP2995948A1 (en) * | 2014-09-09 | 2016-03-16 | Ecole Polytechnique Federale de Lausanne (EPFL) | Methods and compounds useful in hematopoietic stem cell medicine |
JP6958860B2 (en) | 2017-11-07 | 2021-11-02 | 学校法人自治医科大学 | Agents for improving mitochondrial dysfunction, preventive or therapeutic agents for diseases or symptoms caused by mitochondrial dysfunction, and their uses |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996041166A2 (en) * | 1995-06-07 | 1996-12-19 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
-
2000
- 2000-06-22 JP JP2001505191A patent/JP2003506014A/en not_active Withdrawn
- 2000-06-22 WO PCT/US2000/017380 patent/WO2000079274A2/en not_active Application Discontinuation
- 2000-06-22 CA CA002375542A patent/CA2375542A1/en not_active Abandoned
- 2000-06-22 EP EP00943119A patent/EP1210596A2/en not_active Withdrawn
- 2000-06-22 AU AU57636/00A patent/AU5763600A/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996041166A2 (en) * | 1995-06-07 | 1996-12-19 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
Non-Patent Citations (10)
Title |
---|
CACCIATORE TIMOTHY W ET AL: "Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes.", NEURON, vol. 23, no. 3, July 1999 (1999-07-01), pages 449 - 459, XP000961663, ISSN: 0896-6273 * |
CORTESE JORGE D ET AL: "Novel fluorescence membrane fusion assays reveal GTP-dependent fusogenic properties of outer mitochondrial membrane-derived proteins.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1371, no. 2, 28 May 1998 (1998-05-28), pages 185 - 198, XP000961685, ISSN: 0006-3002 * |
DYKENS J A ET AL: "A FRET-based, whole-cell assay of mitochondrial membrane potential for drug screening.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 25, no. 1-2, 1999, 29th Annual Meeting of the Society for Neuroscience, Part 1;Miami Beach, Florida, USA; October 23-28, 1999, pages 282, XP000961632, ISSN: 0190-5295 * |
GONZALEZ JESUS E ET AL: "Improved indicators of cell membrane potential that use fluorescence resonance energy transfer.", CHEMISTRY & BIOLOGY (LONDON), vol. 4, no. 4, 1997, pages 269 - 277, XP000961796, ISSN: 1074-5521 * |
GONZALEZ JESUS E ET AL: "Voltage sensing by fluorescence resonance energy transfer in single cells.", BIOPHYSICAL JOURNAL, vol. 69, no. 4, 1995, pages 1272 - 1280, XP000961694, ISSN: 0006-3495 * |
HUEGLIN D ET AL: "Time-resolved microfluorometric study of the binding sites of lipophilic cationic pyrene probes in mitochondria of living HeLa cells.", JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B BIOLOGY, vol. 31, no. 3, 1995, pages 145 - 158, XP000998251, ISSN: 1011-1344 * |
LEMASTERS JOHN J ET AL: "The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis and autophagy.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1366, no. 1-2, 10 August 1998 (1998-08-10), pages 177 - 196, XP000961686, ISSN: 0006-3002 * |
MAHAJAN N P ET AL: "BCL-2 AND BAX INTERACTIONS IN MITOCHONDRIA PROBED WITH GREEN FLUORESCENT PROTEIN AND FLUORESCENCE RESONANCE ENERGY TRANSFER", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 16, June 1998 (1998-06-01), pages 547 - 552, XP000876894, ISSN: 1087-0156 * |
RIZZUTO R ET AL: "DOUBLE LABELLING OF SUBCELLULAR STRUCTURES WITH ORGANELLE-TARGETED GFP MUTANTS IN VIVO", CURRENT BIOLOGY,GB,CURRENT SCIENCE,, vol. 6, no. 2, 1 February 1996 (1996-02-01), pages 183 - 188, XP000610252, ISSN: 0960-9822 * |
USTER, PAUL S. ET AL: "Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells", J. CELL BIOL. (1986), 103(4), 1221-34, XP000998288 * |
Also Published As
Publication number | Publication date |
---|---|
AU5763600A (en) | 2001-01-09 |
JP2003506014A (en) | 2003-02-18 |
WO2000079274A2 (en) | 2000-12-28 |
EP1210596A2 (en) | 2002-06-05 |
CA2375542A1 (en) | 2000-12-28 |
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