WO2000077510A3 - Procede et dispositif pour electrophorese en gel - Google Patents

Procede et dispositif pour electrophorese en gel Download PDF

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Publication number
WO2000077510A3
WO2000077510A3 PCT/EP2000/005472 EP0005472W WO0077510A3 WO 2000077510 A3 WO2000077510 A3 WO 2000077510A3 EP 0005472 W EP0005472 W EP 0005472W WO 0077510 A3 WO0077510 A3 WO 0077510A3
Authority
WO
WIPO (PCT)
Prior art keywords
separating gel
gel
gel electrophoresis
columns
samples
Prior art date
Application number
PCT/EP2000/005472
Other languages
German (de)
English (en)
Other versions
WO2000077510A2 (fr
Inventor
Holger Rauth
Richard Reinhardt
Antje Starke
Original Assignee
Max Planck Gesellschaft
Holger Rauth
Richard Reinhardt
Antje Starke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Holger Rauth, Richard Reinhardt, Antje Starke filed Critical Max Planck Gesellschaft
Publication of WO2000077510A2 publication Critical patent/WO2000077510A2/fr
Publication of WO2000077510A3 publication Critical patent/WO2000077510A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Selon l'invention, dans un système d'électrophorèse en gel dans lequel une pluralité d'échantillons sont appliqués sous forme matricielle, en rangées et en colonnes droites, sur un support de gel (131) en nappe et parcourent le gel de séparation (130) sous l'effet d'un champ électrique, il est prévu de produire un courant électrique avec au moins deux paires d'électrodes (121,122 et 123,124) comprenant des électrodes disposées sur des bords opposés du gel de séparation, de manière que les échantillons parcourent le gel de séparation conformément à un sens de marche différant des sens des rangées et des colonnes de la configuration desdits échantillons.
PCT/EP2000/005472 1999-06-14 2000-06-14 Procede et dispositif pour electrophorese en gel WO2000077510A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19926985.8 1999-06-14
DE1999126985 DE19926985B4 (de) 1999-06-14 1999-06-14 Verfahren und Vorrichtung zur Gelelektrophorese

Publications (2)

Publication Number Publication Date
WO2000077510A2 WO2000077510A2 (fr) 2000-12-21
WO2000077510A3 true WO2000077510A3 (fr) 2001-03-01

Family

ID=7911122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/005472 WO2000077510A2 (fr) 1999-06-14 2000-06-14 Procede et dispositif pour electrophorese en gel

Country Status (2)

Country Link
DE (1) DE19926985B4 (fr)
WO (1) WO2000077510A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1893991A1 (fr) 2005-06-18 2008-03-05 GE Healthcare Bio-Sciences AB Méthodes et systèmes pour ajouter un réactif à un analyte dans un gel
WO2006136296A1 (fr) * 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Méthode et dispositifs pour former une multitude de puits sur un gel
GB201102385D0 (en) 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2225432A (en) * 1988-02-02 1990-05-30 Inst Molekularbiologie Ak Apparatus for electrophoretic separation of high-molecular dna in gel
WO1996018891A1 (fr) * 1994-12-15 1996-06-20 University College London Electrophorese de matrice en gel
US5785835A (en) * 1996-04-12 1998-07-28 One Lambda Electrophoresis method and devices
WO1999015888A1 (fr) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Appareil et procede d'electrofluidite capillaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4330307C2 (de) * 1993-09-07 1995-11-02 Georg Dr Meyer Verwendung von Nukleinsäuregelelektrophorese zur Identifizierung von Organismenteilen und dergleichen
GB2284484B (en) * 1993-12-03 1997-09-17 Univ London Gel-matrix electrophoresis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2225432A (en) * 1988-02-02 1990-05-30 Inst Molekularbiologie Ak Apparatus for electrophoretic separation of high-molecular dna in gel
WO1996018891A1 (fr) * 1994-12-15 1996-06-20 University College London Electrophorese de matrice en gel
US5785835A (en) * 1996-04-12 1998-07-28 One Lambda Electrophoresis method and devices
WO1999015888A1 (fr) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Appareil et procede d'electrofluidite capillaire

Also Published As

Publication number Publication date
WO2000077510A2 (fr) 2000-12-21
DE19926985B4 (de) 2004-12-02
DE19926985A1 (de) 2001-01-11

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