WO2000077510A3 - Method and device for gel electrophoresis - Google Patents

Method and device for gel electrophoresis Download PDF

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Publication number
WO2000077510A3
WO2000077510A3 PCT/EP2000/005472 EP0005472W WO0077510A3 WO 2000077510 A3 WO2000077510 A3 WO 2000077510A3 EP 0005472 W EP0005472 W EP 0005472W WO 0077510 A3 WO0077510 A3 WO 0077510A3
Authority
WO
WIPO (PCT)
Prior art keywords
separating gel
gel
gel electrophoresis
columns
samples
Prior art date
Application number
PCT/EP2000/005472
Other languages
German (de)
French (fr)
Other versions
WO2000077510A2 (en
Inventor
Holger Rauth
Richard Reinhardt
Antje Starke
Original Assignee
Max Planck Gesellschaft
Holger Rauth
Richard Reinhardt
Antje Starke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Holger Rauth, Richard Reinhardt, Antje Starke filed Critical Max Planck Gesellschaft
Publication of WO2000077510A2 publication Critical patent/WO2000077510A2/en
Publication of WO2000077510A3 publication Critical patent/WO2000077510A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

The invention relates to a gel electrophoresis system in which a number of samples are applied to a flat separating gel in straight rows and columns, in a matrix-type arrangement (131) and run through said separating gel (130) under the influence of an electrical field. At least two electrode pairs (121, 122 or 123, 124) comprising electrodes situated on opposite edges of the separating gel are used to form an electrical current with an effective current direction in such a way that the samples run through the separating gel in a direction that deviates from the directions of the rows and columns of the sample arrangement, accordingly.
PCT/EP2000/005472 1999-06-14 2000-06-14 Method and device for gel electrophoresis WO2000077510A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999126985 DE19926985B4 (en) 1999-06-14 1999-06-14 Method and device for gel electrophoresis
DE19926985.8 1999-06-14

Publications (2)

Publication Number Publication Date
WO2000077510A2 WO2000077510A2 (en) 2000-12-21
WO2000077510A3 true WO2000077510A3 (en) 2001-03-01

Family

ID=7911122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/005472 WO2000077510A2 (en) 1999-06-14 2000-06-14 Method and device for gel electrophoresis

Country Status (2)

Country Link
DE (1) DE19926985B4 (en)
WO (1) WO2000077510A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006261308A1 (en) * 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Method and devices for forming a plurality of wells on a gel
CA2612056A1 (en) 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Methods and systems for adding a reagent to an analyte in a gel
GB201102385D0 (en) 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2225432A (en) * 1988-02-02 1990-05-30 Inst Molekularbiologie Ak Apparatus for electrophoretic separation of high-molecular dna in gel
WO1996018891A1 (en) * 1994-12-15 1996-06-20 University College London Gel-matrix electrophoresis
US5785835A (en) * 1996-04-12 1998-07-28 One Lambda Electrophoresis method and devices
WO1999015888A1 (en) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Capillary electroflow apparatus and method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4330307C2 (en) * 1993-09-07 1995-11-02 Georg Dr Meyer Use of nucleic acid gel electrophoresis to identify organ parts and the like
GB2284484B (en) * 1993-12-03 1997-09-17 Univ London Gel-matrix electrophoresis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2225432A (en) * 1988-02-02 1990-05-30 Inst Molekularbiologie Ak Apparatus for electrophoretic separation of high-molecular dna in gel
WO1996018891A1 (en) * 1994-12-15 1996-06-20 University College London Gel-matrix electrophoresis
US5785835A (en) * 1996-04-12 1998-07-28 One Lambda Electrophoresis method and devices
WO1999015888A1 (en) * 1997-09-19 1999-04-01 Aclara Biosciences, Inc. Capillary electroflow apparatus and method

Also Published As

Publication number Publication date
DE19926985B4 (en) 2004-12-02
DE19926985A1 (en) 2001-01-11
WO2000077510A2 (en) 2000-12-21

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