WO2000073430A3 - Vakzine gegen konformationsabhängige antigene sowie gegen antigene, die keine oder nicht ausschliesslich proteine oder peptide sind - Google Patents

Vakzine gegen konformationsabhängige antigene sowie gegen antigene, die keine oder nicht ausschliesslich proteine oder peptide sind Download PDF

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Publication number
WO2000073430A3
WO2000073430A3 PCT/DE2000/001809 DE0001809W WO0073430A3 WO 2000073430 A3 WO2000073430 A3 WO 2000073430A3 DE 0001809 W DE0001809 W DE 0001809W WO 0073430 A3 WO0073430 A3 WO 0073430A3
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Prior art keywords
peptide
antigens
conformation
peptides
proteins
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PCT/DE2000/001809
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English (en)
French (fr)
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WO2000073430A2 (de
Inventor
Steffen Goletz
Uwe Karsten
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Max Delbrueck Centrum
Steffen Goletz
Uwe Karsten
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Application filed by Max Delbrueck Centrum, Steffen Goletz, Uwe Karsten filed Critical Max Delbrueck Centrum
Priority to EP00951201A priority Critical patent/EP1181058A2/de
Priority to AU64243/00A priority patent/AU6424300A/en
Priority to CA002375033A priority patent/CA2375033A1/en
Publication of WO2000073430A2 publication Critical patent/WO2000073430A2/de
Publication of WO2000073430A3 publication Critical patent/WO2000073430A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4241—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-human or anti-animal Ig
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Die Erfindung betrifft ein Verfahren, das es erlaubt, die hocheffektive Technologie der Vakzinierung mittels Desoxyribonukleinsäure (DNA) nicht nur auf Sequenzepitope von Proteinen oder Peptiden, sondern auch auf Konformationsepitope anzuwenden. Dieses Verfahren ermöglicht darüber hinaus die Nutzung der DNA-Vakzinierung auch bei solchen Antigenen, die keine oder nur teilweise Proteine oder Peptide sind. Die bevorzugte erfindungsgemässe Vakzine enthält als wesentlichen Bestandteil eine Desoxyribonukleinsäure (DNA), die eine Peptidsequenz kodiert, welche ihrerseits die immunologische Imitation (Mimikry) eines konformationsabhängigen Antigens einschließlich Protein-Konformationsepitope oder eines Antigens, das kein oder nur teilweise Protein oder Peptid ist, darstellt. Das Mimikry-Peptid, das ebenfalls Teil der erfindungsgemäßen Vakzine ist oder sein kann, ist entweder ein antiidiotypischer Antikörper, ein Antikörperfragment, ein daraus abgeleitetes Peptid oder ein durch Selektion aus einer Peptid-Genbank erhaltenes spezifisch bindendes Peptid. Anwendungsgebiete der Erfindung sind die medizinische und die veterinärmedizinische Immunologie, darunter die adjuvante Therapie von Tumorerkrankungen.
PCT/DE2000/001809 1999-05-27 2000-05-29 Vakzine gegen konformationsabhängige antigene sowie gegen antigene, die keine oder nicht ausschliesslich proteine oder peptide sind WO2000073430A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00951201A EP1181058A2 (de) 1999-05-27 2000-05-29 Vakzine gegen konformationsabhängige antigene sowie gegen antigene, die keine oder nicht ausschliesslich proteine oder peptide sind
AU64243/00A AU6424300A (en) 1999-05-27 2000-05-29 Vaccines against conformation-dependent antigens and against antigens that are not or are not only proteins or peptides
CA002375033A CA2375033A1 (en) 1999-05-27 2000-05-29 Vaccines against conformation-dependent antigens and against antigens that are not or are not only proteins or peptides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19924405 1999-05-27
DE19924405.7 1999-05-27
DE19943016 1999-09-09
DE19943016.0 1999-09-09

Publications (2)

Publication Number Publication Date
WO2000073430A2 WO2000073430A2 (de) 2000-12-07
WO2000073430A3 true WO2000073430A3 (de) 2001-03-29

Family

ID=26053552

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/001809 WO2000073430A2 (de) 1999-05-27 2000-05-29 Vakzine gegen konformationsabhängige antigene sowie gegen antigene, die keine oder nicht ausschliesslich proteine oder peptide sind

Country Status (5)

Country Link
EP (1) EP1181058A2 (de)
AU (1) AU6424300A (de)
CA (1) CA2375033A1 (de)
DE (1) DE10027695A1 (de)
WO (1) WO2000073430A2 (de)

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DE69434538T2 (de) 1994-03-08 2006-08-10 Human Genome Sciences, Inc. Vaskulärer endothelialer Wachstumsfaktor 2
US7153827B1 (en) 1994-03-08 2006-12-26 Human Genome Sciences, Inc. Vascular endothelial growth factor 2 and methods of use
US6040157A (en) 1994-03-08 2000-03-21 Human Genome Sciences, Inc. Vascular endothelial growth factor 2
US6608182B1 (en) 1994-03-08 2003-08-19 Human Genome Sciences, Inc. Human vascular endothelial growth factor 2
US7186688B1 (en) 1994-03-08 2007-03-06 Human Genome Sciences, Inc. Methods of stimulating angiogenesis in a patient by administering vascular endothelial growth factor 2
US5932540A (en) 1994-03-08 1999-08-03 Human Genome Sciences, Inc. Vascular endothelial growth factor 2
US7109308B1 (en) 1994-03-08 2006-09-19 Human Genome Sciences, Inc. Antibodies to human vascular endothelial growth factor 2
US7223724B1 (en) 1999-02-08 2007-05-29 Human Genome Sciences, Inc. Use of vascular endothelial growth factor to treat photoreceptor cells
US7273751B2 (en) 2000-08-04 2007-09-25 Human Genome Science, Inc. Vascular endothelial growth factor-2
US7402312B2 (en) 2001-04-13 2008-07-22 Human Genome Sciences, Inc. Antibodies to vascular endothelial growth factor 2 (VEGF-2)
DK1385864T3 (da) 2001-04-13 2010-08-16 Human Genome Sciences Inc Anti-VEGF-2-antistoffer
US7658924B2 (en) 2001-10-11 2010-02-09 Amgen Inc. Angiopoietin-2 specific binding agents
US7521053B2 (en) 2001-10-11 2009-04-21 Amgen Inc. Angiopoietin-2 specific binding agents
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
EP1539235A2 (de) 2002-07-01 2005-06-15 Human Genome Sciences, Inc. Spezifisch an reg iv bindende antikörper
SI1529060T1 (sl) 2002-07-22 2015-01-30 Glycotope Gmbh Postopek za proizvodnjo imunostimulacijskih mucinov (MUCI)
EP2317317B1 (de) * 2002-12-03 2014-11-26 North Carolina State University Prionen-Protein-Liganden und Verfahren zu deren Verwendung
GB0324265D0 (en) * 2003-10-16 2003-11-19 Medical Res Council Peptide
US7871782B2 (en) * 2004-03-29 2011-01-18 The University Court Of The University Of Aberdeen Specific binding members against synaptophysin
RU2007139283A (ru) * 2005-03-25 2009-04-27 Гликарт Биотехнологи Аг (Ch) АНТИГЕНСВЯЗЫВАЮЩИЕ МОЛЕКУЛЫ, НАПРАВЛЕННЫЕ К MCSP И ОБЛАДАЮЩИЕ ПОВЫШЕННОЙ АФФИННОСТЬЮ СВЯЗЫВАНИЯ Fc-РЕЦЕПТОРА И ЭФФЕКТОРНОЙ ФУНКЦИЕЙ
EP2365000A3 (de) 2005-05-18 2013-01-16 Ablynx N.V. Verbesserte Nanokörper-TM gegen Tumornekrosefaktor Alpha
DE102005023617A1 (de) 2005-05-21 2006-11-23 Aspre Ag Verfahren zum Mischen von Farben in einem Display
CN1301267C (zh) * 2005-06-21 2007-02-21 中国人民解放军军事医学科学院附属医院 Muc1粘蛋白的一个模拟表位肽及其编码dna与应用
WO2009092806A2 (en) 2008-01-25 2009-07-30 Aarhus Universitet Selective exosite inhibition of papp-a activity against igfbp-4
JO2913B1 (en) 2008-02-20 2015-09-15 امجين إنك, Antibodies directed towards angiopoietin-1 and angiopoietin-2 proteins and their uses
JP2012511032A (ja) 2008-12-05 2012-05-17 アブラクシス バイオサイエンス リミテッド ライアビリティー カンパニー ＳＰＡＲＣ結合ＳｃＦｖ

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Also Published As

Publication number Publication date
WO2000073430A2 (de) 2000-12-07
EP1181058A2 (de) 2002-02-27
DE10027695A1 (de) 2001-04-19
AU6424300A (en) 2000-12-18
CA2375033A1 (en) 2000-12-07

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