WO2000069257A3 - Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques - Google Patents

Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques Download PDF

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Publication number
WO2000069257A3
WO2000069257A3 PCT/US2000/013000 US0013000W WO0069257A3 WO 2000069257 A3 WO2000069257 A3 WO 2000069257A3 US 0013000 W US0013000 W US 0013000W WO 0069257 A3 WO0069257 A3 WO 0069257A3
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WIPO (PCT)
Prior art keywords
vertebrate
transgenic
disclosed
germ cells
male
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PCT/US2000/013000
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English (en)
Other versions
WO2000069257A2 (fr
Inventor
Carol W Readhead
Robert Winston
Original Assignee
Cedars Sinai Medical Center
Imp College Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/311,599 external-priority patent/US6734338B1/en
Application filed by Cedars Sinai Medical Center, Imp College Innovations Ltd filed Critical Cedars Sinai Medical Center
Priority to EP00937536A priority Critical patent/EP1190086A2/fr
Priority to CA002368620A priority patent/CA2368620A1/fr
Priority to AU52688/00A priority patent/AU781014B2/en
Priority to JP2000617726A priority patent/JP2002543814A/ja
Publication of WO2000069257A2 publication Critical patent/WO2000069257A2/fr
Publication of WO2000069257A3 publication Critical patent/WO2000069257A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Diabetes (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Neurology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
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  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Pain & Pain Management (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)

Abstract

Cette invention a trait à une technique in vivo d'incorporation de matériel génétique exogène au génome d'un vertébré, consistant à administrer au testicule de ce vertébré mâle un mélange d'apport de gène comportant un vecteur viral, tel qu'un vecteur rétroviral, pour apporter un polynucléotide codant un trait ou un produit désiré. L'invention porte également sur une technique in vitro d'incorporation de matériel génétique exogène au génome d'un vertébré, les cellules germinales étant obtenues à partir d'un donneur vertébré mâle et modifiées par voie génétique in vitro avant leur transfert vers le vertébré mâle destinataire. Après transfert, le vertébré mâle porteur des cellules germinales modifiées par voie génétique est mis à s'accoupler avec un vertébré femelle de manière à produire une descendance transgénique porteuse du polynucléotide dans son génome. L'invention concerne également des vertébrés transgéniques non humains produits grâce à cette technique, y compris la descendance transgénique. Cette invention porte, en outre, sur une cellule transgénique dérivée du vertébré transgénique, en l'occurrence une cellule germinale, telle qu'un spermatozoïde ou un embryon, une cellule précurseur de l'un ou de l'autre de ces derniers ou une cellule somatique. L'invention concerne, de surcroît, une technique de production de lignée d'animal vertébré transgénique comportant des cellules germinales d'origine porteuses dans leur génome d'au moins un polynucléotide exogène, la semence transgénique contenant les cellules germinales mâles utilisées dans la mise en oeuvre de cette technique.
PCT/US2000/013000 1999-05-13 2000-05-12 Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques WO2000069257A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00937536A EP1190086A2 (fr) 1999-05-13 2000-05-12 Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques
CA002368620A CA2368620A1 (fr) 1999-05-13 2000-05-12 Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques
AU52688/00A AU781014B2 (en) 1999-05-13 2000-05-12 Genetic modification of male germ cells for generation of transgenic species and genetic therapies
JP2000617726A JP2002543814A (ja) 1999-05-13 2000-05-12 トランスジェニック種の発生と遺伝子療法用の雄性生殖細胞の遺伝的修飾法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/311,599 US6734338B1 (en) 1997-11-14 1999-05-13 Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies
US09/311,599 1999-05-13

Publications (2)

Publication Number Publication Date
WO2000069257A2 WO2000069257A2 (fr) 2000-11-23
WO2000069257A3 true WO2000069257A3 (fr) 2001-08-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/013000 WO2000069257A2 (fr) 1999-05-13 2000-05-12 Modification genetique de cellules germinales males pour la production d'especes transgeniques et aux fins de therapies geniques

Country Status (5)

Country Link
EP (1) EP1190086A2 (fr)
JP (1) JP2002543814A (fr)
AU (1) AU781014B2 (fr)
CA (1) CA2368620A1 (fr)
WO (1) WO2000069257A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2814370B1 (fr) * 2000-09-22 2004-08-20 Centre Nat Rech Scient Utilisation d'un complexe acide nucleique/pei pour le ciblage de cellules souches du cerveau
EP1438401B1 (fr) 2001-09-18 2011-06-15 Synageva BioPharma Corp. Production d'oiseaux transgeniques par injection cytoplasmique
US7550650B2 (en) 2001-09-18 2009-06-23 Synageva Biopharma Corp. Production of a transgenic avian by cytoplasmic injection
KR100569168B1 (ko) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 조류 정원줄기세포의 배양방법 및 이에 의해 수득한 조류정원줄기세포
GB0325140D0 (en) * 2003-10-28 2003-12-03 Babraham Inst Methods for selecting gametes and for producing genetically modified non-human animals
JP2007275004A (ja) * 2006-04-10 2007-10-25 Kaneka Corp レンチウイルスベクターの精巣への感染による遺伝子組換え鳥類作製法
GB0802980D0 (en) * 2008-02-19 2008-03-26 Atazoa Ltd Biological materials and uses thereof
CN104994728B (zh) * 2012-10-19 2019-02-15 移卵基因有限公司 用于产生遗传上优质的动物的方法
US20140359796A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
CN111713450B (zh) * 2020-05-26 2022-07-12 上海交通大学医学院 一种慢性粒细胞白血病的pdx模型的建立方法

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EP0867114A1 (fr) * 1995-09-29 1998-09-30 Hoechst Marion Roussel, Ltd. Obtention d'un animal transgenique
WO1999025863A1 (fr) * 1997-11-14 1999-05-27 Cedars-Sinai Medical Center Transfection et transfert de cellules germinales males pour produire des especes transgeniques
WO1999040213A1 (fr) * 1998-02-09 1999-08-12 Tranxenogen, Incorporated Manipulation genetique des spermatogonies
WO2000029601A1 (fr) * 1998-11-13 2000-05-25 Cedars-Sinai Medical Center Technique permettant de depeupler un testicule de vertebre et de generer une espece transgenique
WO2000029602A1 (fr) * 1998-11-13 2000-05-25 Cedars-Sinai Medical Center Transfection de cellules germinales males permettant de generer des cellules souches transgeniques selectionnables

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EP0867114A1 (fr) * 1995-09-29 1998-09-30 Hoechst Marion Roussel, Ltd. Obtention d'un animal transgenique
WO1999025863A1 (fr) * 1997-11-14 1999-05-27 Cedars-Sinai Medical Center Transfection et transfert de cellules germinales males pour produire des especes transgeniques
WO1999040213A1 (fr) * 1998-02-09 1999-08-12 Tranxenogen, Incorporated Manipulation genetique des spermatogonies
WO2000029601A1 (fr) * 1998-11-13 2000-05-25 Cedars-Sinai Medical Center Technique permettant de depeupler un testicule de vertebre et de generer une espece transgenique
WO2000029602A1 (fr) * 1998-11-13 2000-05-25 Cedars-Sinai Medical Center Transfection de cellules germinales males permettant de generer des cellules souches transgeniques selectionnables

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Also Published As

Publication number Publication date
EP1190086A2 (fr) 2002-03-27
AU781014B2 (en) 2005-04-28
WO2000069257A2 (fr) 2000-11-23
CA2368620A1 (fr) 2000-11-23
JP2002543814A (ja) 2002-12-24
AU5268800A (en) 2000-12-05

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