WO2000057180A8 - Utilisation d'analogues nucleosidiques bloques en spectrometrie de masse - Google Patents

Utilisation d'analogues nucleosidiques bloques en spectrometrie de masse

Info

Publication number
WO2000057180A8
WO2000057180A8 PCT/DK2000/000126 DK0000126W WO0057180A8 WO 2000057180 A8 WO2000057180 A8 WO 2000057180A8 DK 0000126 W DK0000126 W DK 0000126W WO 0057180 A8 WO0057180 A8 WO 0057180A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
mass spectrometry
acid molecule
sequence
acid sequence
Prior art date
Application number
PCT/DK2000/000126
Other languages
English (en)
Other versions
WO2000057180A3 (fr
WO2000057180A2 (fr
Inventor
Adrian Simmons
Clifford Smith
Original Assignee
Exiqon As
Adrian Simmons
Clifford Smith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As, Adrian Simmons, Clifford Smith filed Critical Exiqon As
Priority to EP00910582A priority Critical patent/EP1161681A2/fr
Priority to AU32744/00A priority patent/AU3274400A/en
Priority to IL14542100A priority patent/IL145421A0/xx
Priority to JP2000607004A priority patent/JP2002540402A/ja
Publication of WO2000057180A2 publication Critical patent/WO2000057180A2/fr
Publication of WO2000057180A3 publication Critical patent/WO2000057180A3/fr
Publication of WO2000057180A8 publication Critical patent/WO2000057180A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne le diagnostic sur ADN et ARN basé sur la spectrométrie de masse, par exemple, la spectrométrie de masse à désorption-ionisation par impact laser assistée par matrice et saisie en vol (MALDI-TOF), la méthode d'ES-MS, la spectrométrie de masse à résonance ionique cyclotronique (ICR), la spectrométrie de masse par transformation de Fourier, ou des combinaisons de celles-ci, dans lesquelles des sondes d'ADN modifiées partiellement par des analogues nucléosidiques bloqués sont utilisées afin d'augmenter la stabilité et la résolution. L'invention concerne notamment un procédé de détection d'une séquence d'acide nucléique cible d'une molécule d'acide nucléique ou de détection d'une mutation dans une séquence d'acide nucléique d'une molécule d'acide nucléique, dans lequel (a) la molécule d'acide nucléique ou (b) une partie de la molécule d'acide nucléique ou (c) un oligonucléotide complémentaire de la séquence ou au moins une sous-séquence de la molécule d'acide nucléique est analysée par spectrométrie de masse afin d'obtenir des informations directes ou indirectes concernant ladite séquence ou mutation d'acide nucléique, le procédé consistant en l'hybridation d'un oligonucléotide modifié par LNA à la molécule d'acide nucléique.
PCT/DK2000/000126 1999-03-18 2000-03-17 Utilisation d'analogues nucleosidiques bloques en spectrometrie de masse WO2000057180A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00910582A EP1161681A2 (fr) 1999-03-18 2000-03-17 Utilisation d'analogues nucleosidiques bloques en spectrometrie de masse
AU32744/00A AU3274400A (en) 1999-03-18 2000-03-17 Use of lna in mass spectrometry
IL14542100A IL145421A0 (en) 1999-03-18 2000-03-17 Use of lna in mass spectrometry
JP2000607004A JP2002540402A (ja) 1999-03-18 2000-03-17 質量分析法におけるｌｎａの使用

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900381 1999-03-18
DKPA199900381 1999-03-18

Publications (3)

Publication Number Publication Date
WO2000057180A2 WO2000057180A2 (fr) 2000-09-28
WO2000057180A3 WO2000057180A3 (fr) 2001-02-08
WO2000057180A8 true WO2000057180A8 (fr) 2001-05-03

Family

ID=8092860

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000126 WO2000057180A2 (fr) 1999-03-18 2000-03-17 Utilisation d'analogues nucleosidiques bloques en spectrometrie de masse

Country Status (5)

Country Link
EP (1) EP1161681A2 (fr)
JP (1) JP2002540402A (fr)
AU (1) AU3274400A (fr)
IL (1) IL145421A0 (fr)
WO (1) WO2000057180A2 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2217597A (en) * 1996-03-18 1997-10-22 Sequenom, Inc. Dna sequencing by mass spectrometry
US5821060A (en) * 1996-08-02 1998-10-13 Atom Sciences, Inc. DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA
DE19782095T1 (de) * 1996-11-06 2000-03-23 Sequenom Inc DNA-Diagnose auf der Basis von Massenspektrometrie
GB9716231D0 (en) * 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues

Also Published As

Publication number Publication date
WO2000057180A3 (fr) 2001-02-08
AU3274400A (en) 2000-10-09
EP1161681A2 (fr) 2001-12-12
IL145421A0 (en) 2002-06-30
JP2002540402A (ja) 2002-11-26
WO2000057180A2 (fr) 2000-09-28

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