WO2000056768A3 - Chromatographic albumin process - Google Patents

Chromatographic albumin process Download PDF

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Publication number
WO2000056768A3
WO2000056768A3 PCT/US2000/006354 US0006354W WO0056768A3 WO 2000056768 A3 WO2000056768 A3 WO 2000056768A3 US 0006354 W US0006354 W US 0006354W WO 0056768 A3 WO0056768 A3 WO 0056768A3
Authority
WO
WIPO (PCT)
Prior art keywords
caprylate
additional purification
improved method
manufacturing
provides
Prior art date
Application number
PCT/US2000/006354
Other languages
French (fr)
Other versions
WO2000056768A2 (en
Inventor
Rajesh Sharma
Susan Trukawinski
Hanns-Ingolf Paul
Richard Rose
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Priority to AU36244/00A priority Critical patent/AU3624400A/en
Publication of WO2000056768A2 publication Critical patent/WO2000056768A2/en
Publication of WO2000056768A3 publication Critical patent/WO2000056768A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention provides an improved method of manufacturing Albumin utilizing an addition caprylate for both separation and viral inactivation; and chromatography for additional purification. In the improved method the caprylate incubation is carried out at a pH of from about 5.5 to about 5.6 and at a temperature between 30 and 50 degrees centigrade. The required incubation time is decreased to one hour. No centrifugation is necessary, with additional purification to remove unwanted contaminants being accomplished with filtration steps including a chromatographic filtration through an anionic column. This method provides a significant decrease in manufacturing time with increased purity, without any measurable reduction in yield.
PCT/US2000/006354 1999-03-19 2000-03-10 Chromatographic albumin process WO2000056768A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU36244/00A AU3624400A (en) 1999-03-19 2000-03-10 Chromatographic albumin process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27250099A 1999-03-19 1999-03-19
US09/272,500 1999-03-19

Publications (2)

Publication Number Publication Date
WO2000056768A2 WO2000056768A2 (en) 2000-09-28
WO2000056768A3 true WO2000056768A3 (en) 2001-01-04

Family

ID=23040063

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006354 WO2000056768A2 (en) 1999-03-19 2000-03-10 Chromatographic albumin process

Country Status (2)

Country Link
AU (1) AU3624400A (en)
WO (1) WO2000056768A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2221817B1 (en) 2004-07-26 2005-10-01 Probitas Pharma, S.A. "THERAPEUTIC HUMAN ALBUMINE SOLUTIONS WITH LOW ACTIVITY OF THE PRECALICREINE ACTIVATOR (PKA) AND PROCEDURE FOR OBTAINING THEMSELVES".
WO2007079886A1 (en) 2005-12-22 2007-07-19 Csl Behring Gmbh Octanoate-reduced human albumin
AU2013205138B2 (en) * 2012-08-09 2015-06-25 Grifols, S.A. Caprylate Viral Deactivation
AU2016282916B2 (en) * 2015-06-26 2022-01-20 Ferring B.V. Methods of purification and/or viral inactivation
CN113831405B (en) * 2021-11-17 2024-04-30 华兰生物工程重庆有限公司 Purification method of human serum albumin

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452753A1 (en) * 1990-04-19 1991-10-23 Bayer Corporation Essentially monomeric normal serum albumin (human)
US5561115A (en) * 1994-08-10 1996-10-01 Bayer Corporation Low temperature albumin fractionation using sodium caprylate as a partitioning agent

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452753A1 (en) * 1990-04-19 1991-10-23 Bayer Corporation Essentially monomeric normal serum albumin (human)
US5561115A (en) * 1994-08-10 1996-10-01 Bayer Corporation Low temperature albumin fractionation using sodium caprylate as a partitioning agent

Also Published As

Publication number Publication date
AU3624400A (en) 2000-10-09
WO2000056768A2 (en) 2000-09-28

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