WO2000055346A3 - Methodes et materiaux de production elevee et rapide d'une bibliotheque de genes knockouts dans un organisme - Google Patents

Methodes et materiaux de production elevee et rapide d'une bibliotheque de genes knockouts dans un organisme

Info

Publication number
WO2000055346A3
WO2000055346A3 PCT/US2000/007317 US0007317W WO0055346A3 WO 2000055346 A3 WO2000055346 A3 WO 2000055346A3 US 0007317 W US0007317 W US 0007317W WO 0055346 A3 WO0055346 A3 WO 0055346A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector
organism
large insert
rapid
materials
Prior art date
Application number
PCT/US2000/007317
Other languages
English (en)
Other versions
WO2000055346A2 (fr
Inventor
John E Hamer
Lisbeth Hamer
Original Assignee
Paradigm Genetics Inc
John E Hamer
Lisbeth Hamer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc, John E Hamer, Lisbeth Hamer filed Critical Paradigm Genetics Inc
Priority to JP2000605763A priority Critical patent/JP2003502015A/ja
Priority to EP00918144A priority patent/EP1161551A2/fr
Priority to AU39007/00A priority patent/AU3900700A/en
Publication of WO2000055346A2 publication Critical patent/WO2000055346A2/fr
Publication of WO2000055346A3 publication Critical patent/WO2000055346A3/fr
Priority to US09/826,630 priority patent/US6562624B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une méthode permettant de faciliter la recombinaison homologue dirigée vers un site dans un organisme en vue de produire des mutants, et consistant à: 1) construire une bibliothèque de grand vecteur d'insertion comprenant au moins un grand vecteur d'insertion, 2) construire un second vecteur présentant un élément transposable, 3) incuber le produit de l'étape 1) avec le produit de l'étape 2) en présence d'une transposase spécifique des séquences nucléotidiques répétées inversées sur le vecteur de plasmide, 4) amplifier le grand vecteur d'insertion dissocié résultant de l'étape 3, 5) introduire le grand vecteur d'insertion dissocié produit à l'étape 4) dans une cellule hôte cible, et 6) sélectionner systématiquement une recombinaison homologue réussie produite par à l'étape 5) avec le second marqueur sélectionnable.
PCT/US2000/007317 1999-03-17 2000-03-16 Methodes et materiaux de production elevee et rapide d'une bibliotheque de genes knockouts dans un organisme WO2000055346A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000605763A JP2003502015A (ja) 1999-03-17 2000-03-16 生物中における遺伝子ノックアウトライブラリの迅速かつ大量産生のための方法類および素材類
EP00918144A EP1161551A2 (fr) 1999-03-17 2000-03-16 Methodes et materiaux de production elevee et rapide d'une bibliotheque de genes knockouts dans un organisme
AU39007/00A AU3900700A (en) 1999-03-17 2000-03-16 Methods and materials for the rapid and high volume production of a gene knock-out library in an organism
US09/826,630 US6562624B2 (en) 1999-03-17 2001-04-05 Methods and materials for the rapid and high volume production of a gene knock-out library in an organism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27062099A 1999-03-17 1999-03-17
US09/270,620 1999-03-17

Publications (2)

Publication Number Publication Date
WO2000055346A2 WO2000055346A2 (fr) 2000-09-21
WO2000055346A3 true WO2000055346A3 (fr) 2001-02-15

Family

ID=23032091

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/007317 WO2000055346A2 (fr) 1999-03-17 2000-03-16 Methodes et materiaux de production elevee et rapide d'une bibliotheque de genes knockouts dans un organisme

Country Status (4)

Country Link
EP (1) EP1161551A2 (fr)
JP (1) JP2003502015A (fr)
AU (1) AU3900700A (fr)
WO (1) WO2000055346A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611361A (zh) * 2018-05-10 2018-10-02 浙江大学 一种链霉菌生物合成基因簇负调控因子的遗传筛选方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033781B1 (en) 1999-09-29 2006-04-25 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function
WO2001078652A2 (fr) 2000-04-14 2001-10-25 Metabolon, Inc. Procedes permettant de decouvrir des medicaments, de traiter des maladies et de poser des diagnostics a l'aide de la metabolomique
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
EP1294869A2 (fr) * 2000-06-14 2003-03-26 Diversa Corporation Ingenierie cellulaire complete par mutagenese d'une partie substantielle d'un genome de depart, par combinaison de mutations et eventuellement repetition
US20020094536A1 (en) * 2000-12-28 2002-07-18 Cell Therapeutics, Inc. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
WO2003089617A2 (fr) 2002-04-22 2003-10-30 Novozymes Biotech, Inc. Methodes pour augmenter la recombinaison homologue d'une sequence d'acide nucleique
AU2003231228A1 (en) * 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for preparing variants of a dna sequence in filamentous fungi
US8849577B2 (en) 2006-09-15 2014-09-30 Metabolon, Inc. Methods of identifying biochemical pathways
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法

Citations (6)

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Publication number Priority date Publication date Assignee Title
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
WO1997029202A1 (fr) * 1996-02-09 1997-08-14 Het Nederlands Kanker Instituut Vecteurs et methodes afferentes permettant d'apporter a des cellules des acides nucleiques supplementaires s'integrant dans leur genome
WO1998010077A1 (fr) * 1996-09-09 1998-03-12 Wisconsin Alumni Research Foundation Systeme de transposition in vitro utilisant une transposase tn5 modifiee
WO1998056903A1 (fr) * 1997-06-13 1998-12-17 President And Fellows Of Harvard College Methodes et utilisations de ciblage de genes sur la base de transposon
WO1999007871A1 (fr) * 1997-08-12 1999-02-18 Het Nederlands Kanker Instituut Vecteurs et techniques afferentes fournissant des cellules dont le genome renferme un materiau nucleotidique supplementaire
WO1999009817A1 (fr) * 1997-08-22 1999-03-04 Biotechnology And Biological Sciences Research Council Utilisation de l'element mariner pour creer des animaux transgeniques

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
WO1997029202A1 (fr) * 1996-02-09 1997-08-14 Het Nederlands Kanker Instituut Vecteurs et methodes afferentes permettant d'apporter a des cellules des acides nucleiques supplementaires s'integrant dans leur genome
WO1998010077A1 (fr) * 1996-09-09 1998-03-12 Wisconsin Alumni Research Foundation Systeme de transposition in vitro utilisant une transposase tn5 modifiee
WO1998056903A1 (fr) * 1997-06-13 1998-12-17 President And Fellows Of Harvard College Methodes et utilisations de ciblage de genes sur la base de transposon
WO1999007871A1 (fr) * 1997-08-12 1999-02-18 Het Nederlands Kanker Instituut Vecteurs et techniques afferentes fournissant des cellules dont le genome renferme un materiau nucleotidique supplementaire
WO1999009817A1 (fr) * 1997-08-22 1999-03-04 Biotechnology And Biological Sciences Research Council Utilisation de l'element mariner pour creer des animaux transgeniques

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ADACHI KIICHI AND J.E. HAMER: "Divergent cAMP signaling pathways regulate growth and pathogenesis in the rice blast fungus Magnaporthe grisea.", PLANT CELL, vol. 10, no. 8, August 1998 (1998-08-01), pages 1361 - 1373, XP002145186, ISSN: 1040-4651 *
LAMPE DAVID J ET AL: "A purified mariner transposase is sufficient to mediate transposition in vitro.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 15, no. 19, 1996, pages 5470 - 5479, XP002145182, ISSN: 0261-4189 *
LAMPE DAVID J ET AL: "Factors affecting transposition of the Himar1 mariner transposon in vitro.", GENETICS, vol. 149, no. 1, May 1998 (1998-05-01), pages 179 - 187, XP002145183, ISSN: 0016-6731 *
NISHIMURA MARIE ET AL: "Construction of a BAC library of the rice blast fungus Magnaporthe grisea and finding specific genome regions in which its transposons tend to cluster.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 8, August 1998 (1998-08-01), pages 1515 - 1521, XP002145184, ISSN: 0916-8451 *
RUBIN ERIC J ET AL: "In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 4, 16 February 1999 (1999-02-16), Feb. 16, 1999, pages 1645 - 1650, XP002145185, ISSN: 0027-8424 *
S.E. DEVINE ET AL.: "A transposon-based strategy for sequencing repetitive DNA in eucaryotic genomes", GENOME RESEARCH, vol. 7, 1997, CSH LABORATORY PRESS, pages 551 - 563, XP002145187 *
WESTPHAL CHRISTOPH HEINER ET AL: "Transposon-generated 'knock-out' and 'knock-in' gene-targeting constructs for use in mice.", CURRENT BIOLOGY, vol. 7, no. 7, 1997, pages 530 - 533, XP000938425, ISSN: 0960-9822 *
ZHANG L ET AL: "THE HIMAR1 MARINER TRANSPOSASE CLONED IN A RECOMBINANT ADENOVIRUS VECTOR IS FUNCTIONAL IN MAMMALIAN CELLS", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 26, no. 16, August 1998 (1998-08-01), pages 3687 - 3693, XP000700201, ISSN: 0305-1048 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611361A (zh) * 2018-05-10 2018-10-02 浙江大学 一种链霉菌生物合成基因簇负调控因子的遗传筛选方法

Also Published As

Publication number Publication date
EP1161551A2 (fr) 2001-12-12
AU3900700A (en) 2000-10-04
JP2003502015A (ja) 2003-01-21
WO2000055346A2 (fr) 2000-09-21

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